AB81673

Anti-VAP1 antibody [7-88]

Name: Anti-VAP1 antibody [7-88] (ab81673) | Abcam
Brand: Abcam Limited
SKU: AB81673

Be the first to review this product! Submit a review

(7 Publications )

Rat Monoclonal VAP1 antibody. Suitable for IHC-Fr and reacts with Mouse samples. Cited in 7 publications. Immunogen corresponding to Tissue preparation containing Aoc3 protein.

1 Images

Immunohistochemistry (Frozen sections) - Anti-VAP1 antibody [7-88] (AB81673)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-VAP1 antibody [7-88] (AB81673)

ab81673 staining VAP1 in mouse muscle tissue by Immunohistochemistry (Frozen sections).

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

7-88

Isotype

IgG2b

Carrier free

Reacts with

Mouse

Applications

IHC-Fr

applications

Immunogen

Tissue preparation containing Aoc3 protein. The exact immunogen used to generate this antibody is proprietary information.

O70423

style

left-column

Alternative Products

Primary Antibodies

AB196739

Anti-VAP1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-VAP1 antibody (AB196739)

Primary Antibodies

AB115574

Anti-VAP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody (AB115574)

Primary Antibodies

AB81718

Anti-VAP1 antibody [174-5]

Immunohistochemistry (Frozen sections) - Anti-VAP1 antibody [174-5] (AB81718)

Primary Antibodies

AB187202

Anti-VAP1 antibody

Western blot - Anti-VAP1 antibody (AB187202)

Primary Antibodies

AB42885

Anti-VAP1 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody (AB42885)

Complementary Products

Assay Kits

AB243684

Human VAP-1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human VAP-1 ELISA Kit (AB243684)

Sample Prep & Detection Kits

AB188285

FITC Conjugation Kit (Fast) - Lightning-Link®

Fluorescence Microscopy - FITC Conjugation Kit (Fast) - Lightning-Link® (AB188285)

Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Sample Prep & Detection Kits

AB201807

APC Conjugation Kit - Lightning-Link®

Fluorescence Microscopy - APC Conjugation Kit - Lightning-Link® (AB201807)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "<p></p>" } } }

style

left-column

Product details

Inhibits migration of granulocytes and monocytes in acute models of inflammation.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Vascular Adhesion Protein 1 (VAP1) also known as amine oxidase copper-containing 3 (AOC3) has a mechanical function involving its enzyme activity to catalyze oxidative deamination of primary amines to produce aldehydes hydrogen peroxide and ammonia. The protein has an approximate mass of 90 kDa. VAP1 is expressed on the surface of endothelial cells and some smooth muscle cells especially in tissues like liver lung and gut. It functions mainly in cellular adhesion processes.

Biological function summary

VAP1 plays important roles in leukocyte trafficking what makes it important for immune response and inflammation. It facilitates the adhesion and transmigration of leukocytes across the endothelium into tissues. VAP1 is part of numerous adhesion molecule networks that help regulate the immune response. Its ability to interact with diverse substrates also affects vascular function and integrity.

Pathways

VAP1 interacts with immune responses and inflammation-related pathways. It influences the leukocyte adhesion cascade which integrates with the immune cell signaling pathways. Proteins like Integrins and Selectins relate to VAP1 in these processes as they are also involved in mediating cell adhesion to the endothelial cells thereby orchestrating cell movement and positioning essential for immune defense.

VAP1 has been implicated in chronic inflammatory diseases and certain cancers. Inflammatory bowel disease is one condition where VAP1's role in leukocyte trafficking results in sustained inflammation. Additionally its elevated expression in some tumors links it to cancer progression via promoting tumor-associated inflammation. The connection between VAP1 and proteins like TNF-alpha becomes clearer in the inflammation context serving as a potential therapeutic target for modulating inflammatory and cancerous conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the oxidative deamination of primary amines to the corresponding aldehydes with the concomitant production of hydrogen peroxide and ammonia. Has a preference for the primary monoamines methylamine and benzylamine. Could also act on 2-phenylethylamine but much less efficiently. At endothelial cells surface can also function as a cell adhesion protein that participates in lymphocyte extravasation and recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion.

See full target information Aoc3

Alternative Names

Vap1, Amine oxidase [copper-containing] 3, Amine oxidase copper-containing 3, Copper amine oxidase, Semicarbazide-sensitive amine oxidase, Vascular adhesion protein 1, SSAO, VAP-1

style

left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature metabolism 4:90-105 PubMed35027768

2022

Identification of a regulatory pathway inhibiting adipogenesis via RSPO2.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Dong,Wenfei Sun,Yang Shen,Miroslav Baláz,Lucia Balázová,Lianggong Ding,Mona Löffler,Bradford Hamilton,Nora Klöting,Matthias Blüher,Heike Neubauer,Holger Klein,Christian Wolfrum

Oncology reports 44:1627-1637 PubMed32945512

2020

MicroRNA‑137 exerts protective effects on hypoxia‑induced cell injury by inhibiting autophagy/mitophagy and maintaining mitochondrial function in breast cancer stem‑like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qiongying Hu,Yun Yuan,Yeke Wu,Yongliang Huang,Ziyi Zhao,Chong Xiao

Nature 559:103-108 PubMed29925944

2018

A stromal cell population that inhibits adipogenesis in mammalian fat depots.

Applications

Unspecified application

Species

Unspecified reactive species

Petra C Schwalie,Hua Dong,Magda Zachara,Julie Russeil,Daniel Alpern,Nassila Akchiche,Christian Caprara,Wenfei Sun,Kai-Uwe Schlaudraff,Gianni Soldati,Christian Wolfrum,Bart Deplancke

Molecular vision 18:593-600 PubMed22419852

2012

Blockade of vascular adhesion protein-1 attenuates choroidal neovascularization.

Applications

ICC/IF

Species

Mouse

Nami Yoshikawa,Kousuke Noda,Yoko Ozawa,Kazuo Tsubota,Yukihiko Mashima,Susumu Ishida

Immunity 23:153-63 PubMed16111634

2005

Rules of recruitment for Th1 and Th2 lymphocytes in inflamed liver: a role for alpha-4 integrin and vascular adhesion protein-1.

Applications

IHC-Fr

Species

Mouse

Claudine S Bonder,M Ursula Norman,Mark G Swain,Lori D Zbytnuik,Jun Yamanouchi,Pere Santamaria,Maureen Ajuebor,Marko Salmi,Sirpa Jalkanen,Paul Kubes

The American journal of pathology 166:793-800 PubMed15743791

2005

Vascular adhesion protein-1 is involved in both acute and chronic inflammation in the mouse.

Applications

Flow Cyt, IHC-Fr, FuncS

Species

Mouse, Mouse, Mouse

Marika Merinen,Heikki Irjala,Marko Salmi,Ilkka Jaakkola,Arno Hänninen,Sirpa Jalkanen

Diabetologia 47:429-438 PubMed14968297

2004

Adipocytes release a soluble form of VAP-1/SSAO by a metalloprotease-dependent process and in a regulated manner.

Applications

Species

Mouse

A Abella,S García-Vicente,N Viguerie,A Ros-Baró,M Camps,M Palacín,A Zorzano,L Marti

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com