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AB196739

Anti-VAP1 antibody

5

(1 Review)

|

(2 Publications)

Rabbit Polyclonal VAP1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AOC3.

View Alternative Names

VAP1, AOC3, Amine oxidase [copper-containing] 3, Amine oxidase copper-containing 3, Copper amine oxidase, HPAO, Semicarbazide-sensitive amine oxidase, Vascular adhesion protein 1, SSAO, VAP-1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-VAP1 antibody (AB196739)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VAP1 antibody (AB196739)

Immunofluorescent analysis of A549 cells labeling VAP1 using ab196739 at 1/50 dilution.

Blue : DAPI for nuclear staining.

Western blot - Anti-VAP1 antibody (AB196739)
  • WB

Supplier Data

Western blot - Anti-VAP1 antibody (AB196739)

All lanes:

Western blot - Anti-VAP1 antibody (ab196739) at 1/500 dilution

Lane 1:

mouse kidney tissue lysate

Lane 2:

mouse liver tissue lysate

Lane 3:

mouse heart tissue lysate

Predicted band size: 85 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human AOC3. The exact immunogen used to generate this antibody is proprietary information.

Q16853

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Vascular Adhesion Protein 1 (VAP1) also known as amine oxidase copper-containing 3 (AOC3) has a mechanical function involving its enzyme activity to catalyze oxidative deamination of primary amines to produce aldehydes hydrogen peroxide and ammonia. The protein has an approximate mass of 90 kDa. VAP1 is expressed on the surface of endothelial cells and some smooth muscle cells especially in tissues like liver lung and gut. It functions mainly in cellular adhesion processes.
Biological function summary

VAP1 plays important roles in leukocyte trafficking what makes it important for immune response and inflammation. It facilitates the adhesion and transmigration of leukocytes across the endothelium into tissues. VAP1 is part of numerous adhesion molecule networks that help regulate the immune response. Its ability to interact with diverse substrates also affects vascular function and integrity.

Pathways

VAP1 interacts with immune responses and inflammation-related pathways. It influences the leukocyte adhesion cascade which integrates with the immune cell signaling pathways. Proteins like Integrins and Selectins relate to VAP1 in these processes as they are also involved in mediating cell adhesion to the endothelial cells thereby orchestrating cell movement and positioning essential for immune defense.

VAP1 has been implicated in chronic inflammatory diseases and certain cancers. Inflammatory bowel disease is one condition where VAP1's role in leukocyte trafficking results in sustained inflammation. Additionally its elevated expression in some tumors links it to cancer progression via promoting tumor-associated inflammation. The connection between VAP1 and proteins like TNF-alpha becomes clearer in the inflammation context serving as a potential therapeutic target for modulating inflammatory and cancerous conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the oxidative deamination of primary amines to the corresponding aldehydes with the concomitant production of hydrogen peroxide and ammonia (PubMed : 19588076, PubMed : 24304424, PubMed : 9653080). Has a preference for the primary monoamines methylamine and benzylamine (PubMed : 19588076, PubMed : 9653080). Could also act on 2-phenylethylamine but much less efficiently (PubMed : 19588076). At endothelial cells surface can also function as a cell adhesion protein that participates in lymphocyte extravasation and recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion (PubMed : 9254657, PubMed : 9653080).. Isoform 2. Has no semicarbazide-sensitive amine oxidase (SSAO) activity.
See full target information AOC3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Artificial cells, nanomedicine, and biotechnology 48:188-196 PubMed31865777

2019

Silencing hsa_circ_PVT1 (circPVT1) suppresses the growth and metastasis of glioblastoma multiforme cells by up-regulation of miR-199a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Guonan Chi,Fuwei Yang,Donghui Xu,Weiming Liu

Molecular vision 23:853-871 PubMed29259392

2017

Association of HMGB1 with oxidative stress markers and regulators in PDR.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed M Abu El-Asrar,Kaiser Alam,Marta Garcia-Ramirez,Ajmal Ahmad,Mohammad Mairaj Siddiquei,Ghulam Mohammad,Ahmed Mousa,Gert De Hertogh,Ghislain Opdenakker,Rafael Simó
View all publications
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