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AB259525

Anti-VAP1 antibody [EPR21855-125] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal VAP1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

VAP1, AOC3, Amine oxidase [copper-containing] 3, Amine oxidase copper-containing 3, Copper amine oxidase, HPAO, Semicarbazide-sensitive amine oxidase, Vascular adhesion protein 1, SSAO, VAP-1

1 Images
Sandwich ELISA - Anti-VAP1 antibody [EPR21855-125] - BSA and Azide free (Capture) (AB259525)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-VAP1 antibody [EPR21855-125] - BSA and Azide free (Capture) (AB259525)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab243684).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21855-125

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab259525 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human VAP-1. The recommended pair for sandwich ELISA is:
Capture: ab259525, Human VAP-1 Capture Antibody (unconjugated)
Detector: ab259526, Human VAP-1 Detector Antibody (unconjugated)
The reference range value is 31.25 - 2000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Vascular Adhesion Protein 1 (VAP1) also known as amine oxidase copper-containing 3 (AOC3) has a mechanical function involving its enzyme activity to catalyze oxidative deamination of primary amines to produce aldehydes hydrogen peroxide and ammonia. The protein has an approximate mass of 90 kDa. VAP1 is expressed on the surface of endothelial cells and some smooth muscle cells especially in tissues like liver lung and gut. It functions mainly in cellular adhesion processes.
Biological function summary

VAP1 plays important roles in leukocyte trafficking what makes it important for immune response and inflammation. It facilitates the adhesion and transmigration of leukocytes across the endothelium into tissues. VAP1 is part of numerous adhesion molecule networks that help regulate the immune response. Its ability to interact with diverse substrates also affects vascular function and integrity.

Pathways

VAP1 interacts with immune responses and inflammation-related pathways. It influences the leukocyte adhesion cascade which integrates with the immune cell signaling pathways. Proteins like Integrins and Selectins relate to VAP1 in these processes as they are also involved in mediating cell adhesion to the endothelial cells thereby orchestrating cell movement and positioning essential for immune defense.

VAP1 has been implicated in chronic inflammatory diseases and certain cancers. Inflammatory bowel disease is one condition where VAP1's role in leukocyte trafficking results in sustained inflammation. Additionally its elevated expression in some tumors links it to cancer progression via promoting tumor-associated inflammation. The connection between VAP1 and proteins like TNF-alpha becomes clearer in the inflammation context serving as a potential therapeutic target for modulating inflammatory and cancerous conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the oxidative deamination of primary amines to the corresponding aldehydes with the concomitant production of hydrogen peroxide and ammonia (PubMed : 19588076, PubMed : 24304424, PubMed : 9653080). Has a preference for the primary monoamines methylamine and benzylamine (PubMed : 19588076, PubMed : 9653080). Could also act on 2-phenylethylamine but much less efficiently (PubMed : 19588076). At endothelial cells surface can also function as a cell adhesion protein that participates in lymphocyte extravasation and recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion (PubMed : 9254657, PubMed : 9653080).. Isoform 2. Has no semicarbazide-sensitive amine oxidase (SSAO) activity.
See full target information AOC3
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