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Rabbit Recombinant Monoclonal VAP1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (AB317620), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (AB317620), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (AB317620), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (AB317620), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (AB317620), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow CytIPIHC-P
Human
Tested
Not recommended
Not recommended
Not recommended
Tested
Mouse
Tested
Not recommended
Not recommended
Not recommended
Tested
Rat
Tested
Not recommended
Not recommended
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Catalyzes the oxidative deamination of primary amines to the corresponding aldehydes with the concomitant production of hydrogen peroxide and ammonia (PubMed:19588076, PubMed:24304424, PubMed:9653080). Has a preference for the primary monoamines methylamine and benzylamine (PubMed:19588076, PubMed:9653080). Could also act on 2-phenylethylamine but much less efficiently (PubMed:19588076). At endothelial cells surface can also function as a cell adhesion protein that participates in lymphocyte extravasation and recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion (PubMed:9254657, PubMed:9653080). Isoform 2. Has no semicarbazide-sensitive amine oxidase (SSAO) activity.

Alternative names

VAP1, AOC3, Amine oxidase [copper-containing] 3, Amine oxidase copper-containing 3, Copper amine oxidase, HPAO, Semicarbazide-sensitive amine oxidase, Vascular adhesion protein 1, SSAO, VAP-1

Recommended products

Rabbit Recombinant Monoclonal VAP1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28748-59
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Vascular Adhesion Protein 1 (VAP1) also known as amine oxidase copper-containing 3 (AOC3) has a mechanical function involving its enzyme activity to catalyze oxidative deamination of primary amines to produce aldehydes hydrogen peroxide and ammonia. The protein has an approximate mass of 90 kDa. VAP1 is expressed on the surface of endothelial cells and some smooth muscle cells especially in tissues like liver lung and gut. It functions mainly in cellular adhesion processes.

Biological function summary

VAP1 plays important roles in leukocyte trafficking what makes it important for immune response and inflammation. It facilitates the adhesion and transmigration of leukocytes across the endothelium into tissues. VAP1 is part of numerous adhesion molecule networks that help regulate the immune response. Its ability to interact with diverse substrates also affects vascular function and integrity.

Pathways

VAP1 interacts with immune responses and inflammation-related pathways. It influences the leukocyte adhesion cascade which integrates with the immune cell signaling pathways. Proteins like Integrins and Selectins relate to VAP1 in these processes as they are also involved in mediating cell adhesion to the endothelial cells thereby orchestrating cell movement and positioning essential for immune defense.

Associated diseases and disorders

VAP1 has been implicated in chronic inflammatory diseases and certain cancers. Inflammatory bowel disease is one condition where VAP1's role in leukocyte trafficking results in sustained inflammation. Additionally its elevated expression in some tumors links it to cancer progression via promoting tumor-associated inflammation. The connection between VAP1 and proteins like TNF-alpha becomes clearer in the inflammation context serving as a potential therapeutic target for modulating inflammatory and cancerous conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620)

    Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling VAP1 with ab317620 at 1/1000 (0.519 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on endothelium of rat lung.

    The section was incubated with ab317620 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling VAP1 with ab317620 at 1/1000 (0.519 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on smooth muscle and endothelium of mouse colon.

    The section was incubated with ab317620 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling VAP1 with ab317620 at 1/1000 (0.519 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on endothelium of human skeletal muscle (PMID: 20154208).

    The section was incubated with ab317620 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling VAP1 with ab317620 at 1/1000 (0.519 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on smooth muscle and endothelium of human colon (PMID: 26912327).

    The section was incubated with ab317620 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (ab317620)

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling VAP1 with ab317620 at 1/1000 (0.519 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on endothelium of human lung.

    The section was incubated with ab317620 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-VAP1 antibody [EPR28748-59] (ab317620), expandable thumbnail

    Western blot - Anti-VAP1 antibody [EPR28748-59] (ab317620)

    Low expression: skeletal muscle (PMID: 23349812).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-VAP1 antibody [EPR28748-59] (ab317620) at 1/1000 dilution

    Lane 1: Mouse lung tissue lysate at 20 µg with NFDM/TBST

    Lane 2: Mouse skeletal muscle tissue lysate at 20 µg with NFDM/TBST

    Lane 3: Mouse colon tissue lysate at 20 µg with NFDM/TBST

    Lane 4: Rat lu tissue lysate at 20 µg with NFDM/TBST

    Lane 5: Rat skeletal muscle tissue lysate at 20 µg with NFDM/TBST

    Lane 6: Rat colon tissue lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 100 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-VAP1 antibody [EPR28748-59] (ab317620), expandable thumbnail

    Western blot - Anti-VAP1 antibody [EPR28748-59] (ab317620)

    Low expression: skeletal muscle (PMID: 23349812).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-3: 15 seconds; Lane 4: 81 seconds.

    All lanes: Western blot - Anti-VAP1 antibody [EPR28748-59] (ab317620) at 1/1000 dilution

    Lane 1: Human lung tissue lysate at 10 µg with NFDM/TBST

    Lane 2: Human skeletal muscle tissue lysate at 10 µg with NFDM/TBST

    Lane 3: Human colon tissue lysate at 10 µg with NFDM/TBST

    Lane 4: Human liver tissue lysate at 10 µg with NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 100 kDa, 36 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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