Anti-VAP1 antibody [EPR28748-59]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (AB317620)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling VAP1 with ab317620 at 1/1000 (0.519 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on smooth muscle and endothelium of human colon (PMID : 26912327).
The section was incubated with ab317620 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-VAP1 antibody [EPR28748-59] (AB317620)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver tissue staining Carboxypeptidase A with ab317620 at a 1/1000 dilution, ab322202 anti-Inhibin beta E chain used at 1/2000 dilution and ab52625 anti-Cytokeratin 19 used at a 1/6000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-VAP1 (green; Opal^™520), anti-Inhibin beta E chain (magenta; Opal^™690) and anti-Cytokeratin 19 (gray; Opal^™570) on human liver. Panel B : anti-VAP1 staining endothelium in human liver.
Panel C : anti-Inhibin beta E chain staining hepatocytes in human liver.
Panel D : anti-Cytokeratin 19 staining branch of bile ducts in human liver.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab317620, ab322202 and ab52625 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (AB317620)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling VAP1 with ab317620 at 1/1000 (0.519 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on endothelium of human lung.
The section was incubated with ab317620 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (AB317620)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling VAP1 with ab317620 at 1/1000 (0.519 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on endothelium of human skeletal muscle (PMID : 20154208).
The section was incubated with ab317620 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (AB317620)

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling VAP1 with ab317620 at 1/1000 (0.519 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on endothelium of rat lung.
The section was incubated with ab317620 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAP1 antibody [EPR28748-59] (AB317620)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling VAP1 with ab317620 at 1/1000 (0.519 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on smooth muscle and endothelium of mouse colon.
The section was incubated with ab317620 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-VAP1 antibody [EPR28748-59] (AB317620)

Low expression : skeletal muscle (PMID : 23349812).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-3 : 15 seconds; Lane 4 : 81 seconds.

All lanes:

Western blot - Anti-VAP1 antibody [EPR28748-59] (ab317620) at 1/1000 dilution

Lane 1:

Human lung tissue lysate at 10 µg

Lane 2:

Human skeletal muscle tissue lysate at 10 µg

Lane 3:

Human colon tissue lysate at 10 µg

Lane 4:

Human liver tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 100 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-VAP1 antibody [EPR28748-59] (AB317620)

Low expression : skeletal muscle (PMID : 23349812).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-VAP1 antibody [EPR28748-59] (ab317620) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 20 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 3:

Mouse colon tissue lysate at 20 µg

Lane 4:

Rat lu tissue lysate at 20 µg

Lane 5:

Rat skeletal muscle tissue lysate at 20 µg

Lane 6:

Rat colon tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 100 kDa,36 kDa

false

Exposure time: 180s