Anti-VAPA antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody (AB225890)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for VAPA using ab225890 at 1/1000 dilution in immunohistochemical analysis.

Detection : DAB staining.

• IP

Supplier Data

Immunoprecipitation - Anti-VAPA antibody (AB225890)

VAPA was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN buffer; 1 mg for IP, 20% of IP loaded) with ab225890 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225890 at 1 μg/ml.

Lane 1 : ab225890 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-VAPA antibody (ab225890)

Predicted band size: 28 kDa

false

• WB

Supplier Data

Western blot - Anti-VAPA antibody (AB225890)

All lanes:

Western blot - Anti-VAPA antibody (ab225890) at 0.4 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate prepared using NETN lysis buffer at 50 µg

Lane 2:

HeLa whole cell lysate prepared using RIPA lysis buffer at 50 µg

Lane 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate prepared using NETN lysis buffer at 50 µg

Lane 4:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate prepared using NETN lysis buffer at 50 µg

Lane 5:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate prepared using NETN lysis buffer at 50 µg

Predicted band size: 28 kDa

true

Exposure time: 30s