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AB244312

Anti-VAPA antibody

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Rabbit Polyclonal VAPA antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human VAPA aa 100 to C-terminus.

View Alternative Names

VAP33, VAPA, Vesicle-associated membrane protein-associated protein A, VAMP-A, VAMP-associated protein A, VAP-A, 33 kDa VAMP-associated protein, VAP-33

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody (AB244312)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody (AB244312)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for VAPA using ab244312 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-VAPA antibody (AB244312)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-VAPA antibody (AB244312)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for VAPA (green) using ab244312 at 4 μg/ml in ICC/IF.

Western blot - Anti-VAPA antibody (AB244312)
  • WB

Unknown

Western blot - Anti-VAPA antibody (AB244312)

All lanes:

Western blot - Anti-VAPA antibody (ab244312) at 0.4 µg/mL

All lanes:

MOLT-4 (human lymphoblastic leukemia cell line) cell lysate

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human VAPA aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9P0L0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Vesicle-associated membrane protein-associated protein A (VAPA) also known as VAP-A is an integral membrane protein involved in intracellular membrane trafficking. It has a molecular weight of approximately 33 kDa and is expressed in multiple tissues including the brain liver and muscles. Mechanically VAPA functions in the process of vesicle docking and fusion at membranes which is essential for the trafficking of proteins and lipids within cells. This protein is also a part of the endoplasmic reticulum (ER) and contributes to the formation of membrane contact sites between the ER and other organelles.
Biological function summary

In the cellular environment VAPA plays an important role in maintaining homeostasis by regulating lipid transport and metabolism. It forms a complex with several other proteins such as OSBP (oxysterol-binding protein) and CERT (ceramide transport protein) which facilitates the exchange of lipids between the ER and the Golgi apparatus. This interaction underlines VAPA’s role in lipid droplet dynamics and the synthesis of phosphatidylinositols which are critical for cell signaling and membrane integrity.

Pathways

The involvement of VAPA is significant in lipid metabolism and membrane trafficking pathways. It interacts with proteins like VAMP (vesicle-associated membrane proteins) and SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complexes which are important for exocytosis and endocytosis processes. VAPA's interaction in these pathways supports the movement of materials across cellular membranes and its regulation of lipid exchange impacts the signaling pathways influencing cell growth and differentiation.

Abnormal VAPA function relates to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and some forms of motor neuron disease. In such conditions VAPA’s interaction with proteins like TDP-43 (TAR DNA-binding protein 43) becomes disrupted leading to protein aggregation and neuronal toxicity. Additionally disruptions in VAPA-associated lipid transport are linked to metabolic disorders like type 2 diabetes highlighting its critical role in proper cellular communication and metabolic balance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44 (PubMed : 32344433, PubMed : 33124732). STARD3-VAPA interaction enables cholesterol transfer from the ER to endosomes (PubMed : 33124732). Via interaction with WDR44 participates in neosynthesized protein export (PubMed : 32344433). In addition, recruited to the plasma membrane through OSBPL3 binding (PubMed : 25447204). The OSBPL3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface (PubMed : 25447204). With OSBPL3, may regulate ER morphology (PubMed : 16143324). May play a role in vesicle trafficking (PubMed : 11511104, PubMed : 19289470).
See full target information VAPA
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Product promise

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