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AB103638

Anti-VAPB antibody

3

(2 Reviews)

|

(3 Publications)

Rabbit Polyclonal VAPB antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VAPB.

View Alternative Names

UNQ484/PRO983, VAPB, Vesicle-associated membrane protein-associated protein B/C, VAMP-B/VAMP-C, VAMP-associated protein B/C, VAP-B/VAP-C

3 Images
Western blot - Anti-VAPB antibody (AB103638)
  • WB

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Western blot - Anti-VAPB antibody (AB103638)

All lanes:

Western blot - Anti-VAPB antibody (ab103638) at 1/500 dilution

All lanes:

HepG2 cell lysate at 50 µg

Predicted band size: 27 kDa

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Western blot - Anti-VAPB antibody (AB103638)
  • WB

Unknown

Western blot - Anti-VAPB antibody (AB103638)

All lanes:

Western blot - Anti-VAPB antibody (ab103638) at 1/500 dilution

All lanes:

Mouse lung tissue lysate at 50 µg

Predicted band size: 27 kDa

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Western blot - Anti-VAPB antibody (AB103638)
  • WB

Unknown

Western blot - Anti-VAPB antibody (AB103638)

All lanes:

Western blot - Anti-VAPB antibody (ab103638) at 1/500 dilution

Lane 1:

VAPB transfected 293T cell line lysate at 25 µg

Lane 2:

Non-transfected 293T cell line lysate at 25 µg

Predicted band size: 27 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human VAPB.

O95292

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VAMP-associated protein B (VAPB) also known as VAP-B is a protein with a mass of approximately 27 kDa. It plays a role in membrane trafficking and lipid transport. VAPB accomplishes this by interacting with various vesicle-associated membrane protein (VAMP) family members. It is expressed in multiple tissues including the brain liver and muscle. VAPB localizes to the endoplasmic reticulum where it facilitates its mechanical functions.
Biological function summary

The protein assists in maintaining cellular homeostasis by influencing lipid transport and membrane composition. VAPB forms part of a larger protein complex that includes other lipid-binding proteins and is essential for the regulation of lipid-dependent signaling pathways. This complex allows VAPB to perform its biological functions effectively within cells.

Pathways

VAPB participates in critical pathways like the unfolded protein response (UPR) and ER-associated degradation (ERAD). These pathways ensure proper protein folding and degradation. VAPB interacts with other important proteins such as VAMP family members to convey signals and manage lipid distribution. Disruptions in these pathways can affect cellular functions evidencing VAPB's integral role.

VAPB has strong connections to certain neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA). Mutations in the VAPB gene have been linked to the familial form of ALS impacting the protein's function and leading to disease progression. VAPB associates with proteins like TDP-43 which also play a role in neurodegenerative disease mechanisms establishing its critical involvement in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44 (PubMed : 32344433, PubMed : 33124732). Interacts with STARD3 in a FFAT motif phosphorylation dependent manner (PubMed : 33124732). Via interaction with WDR44 participates in neosynthesized protein export (PubMed : 32344433). Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity (PubMed : 16891305, PubMed : 20940299). Involved in cellular calcium homeostasis regulation (PubMed : 22131369).
See full target information VAPB
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 20:e46451 PubMed32048434

2019

Signal peptide peptidase-like 2c impairs vesicular transport and cleaves SNARE proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Alkmini A Papadopoulou,Stephan A Müller,Torben Mentrup,Merav D Shmueli,Johannes Niemeyer,Martina Haug-Kröper,Julia von Blume,Artur Mayerhofer,Regina Feederle,Bernd Schröder,Stefan F Lichtenthaler,Regina Fluhrer

EMBO reports 20: PubMed30733281

2019

Signal Peptide Peptidase-Like 2c (SPPL2c) impairs vesicular transport and cleavage of SNARE proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Alkmini A Papadopoulou,Stephan A Müller,Torben Mentrup,Merav D Shmueli,Johannes Niemeyer,Martina Haug-Kröper,Julia von Blume,Artur Mayerhofer,Regina Feederle,Bernd Schröder,Stefan F Lichtenthaler,Regina Fluhrer

Journal of neuropathology and experimental neurology 72:524-39 PubMed23656994

2013

Functional genomics reveals dysregulation of cortical olfactory receptors in Parkinson disease: novel putative chemoreceptors in the human brain.

Applications

Unspecified application

Species

Unspecified reactive species

Paula Garcia-Esparcia,Agatha Schlüter,Margarita Carmona,Jesús Moreno,Belen Ansoleaga,Benjamín Torrejón-Escribano,Stefano Gustincich,Aurora Pujol,Isidre Ferrer
View all publications
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