AB196487

Anti-VAPB antibody

Name: Anti-VAPB antibody (ab196487) | Abcam
Brand: Abcam Limited
SKU: AB196487

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(3 Publications)

Rabbit Polyclonal VAPB antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human VAPB.

View Alternative Names

UNQ484/PRO983, VAPB, Vesicle-associated membrane protein-associated protein B/C, VAMP-B/VAMP-C, VAMP-associated protein B/C, VAP-B/VAP-C

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-VAPB antibody (AB196487)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VAPB antibody (AB196487)

Immunofluorescence analysis of HeLa cells labeling VAPB (green) with ab196487 at 1/50 dilution. Nuclei were counterstained with DAPI (blue).

Supplier Data

Western blot - Anti-VAPB antibody (AB196487)

All lanes:

Western blot - Anti-VAPB antibody (ab196487) at 1/500 dilution

Lane 1:

BT474 cell extract

Lane 2:

SW480 cell extract

Lane 3:

HepG2 cell extract

Lane 4:

H460 cell extract

Lane 5:

Raji cell extract

Lane 6:

Mouse brain tissue extract

Lane 7:

Mouse intestine tissue extract

Predicted band size: 27 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human VAPB. The exact immunogen used to generate this antibody is proprietary information.

O95292

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VAMP-associated protein B (VAPB) also known as VAP-B is a protein with a mass of approximately 27 kDa. It plays a role in membrane trafficking and lipid transport. VAPB accomplishes this by interacting with various vesicle-associated membrane protein (VAMP) family members. It is expressed in multiple tissues including the brain liver and muscle. VAPB localizes to the endoplasmic reticulum where it facilitates its mechanical functions.

Biological function summary

The protein assists in maintaining cellular homeostasis by influencing lipid transport and membrane composition. VAPB forms part of a larger protein complex that includes other lipid-binding proteins and is essential for the regulation of lipid-dependent signaling pathways. This complex allows VAPB to perform its biological functions effectively within cells.

Pathways

VAPB participates in critical pathways like the unfolded protein response (UPR) and ER-associated degradation (ERAD). These pathways ensure proper protein folding and degradation. VAPB interacts with other important proteins such as VAMP family members to convey signals and manage lipid distribution. Disruptions in these pathways can affect cellular functions evidencing VAPB's integral role.

VAPB has strong connections to certain neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA). Mutations in the VAPB gene have been linked to the familial form of ALS impacting the protein's function and leading to disease progression. VAPB associates with proteins like TDP-43 which also play a role in neurodegenerative disease mechanisms establishing its critical involvement in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44 (PubMed : 32344433, PubMed : 33124732). Interacts with STARD3 in a FFAT motif phosphorylation dependent manner (PubMed : 33124732). Via interaction with WDR44 participates in neosynthesized protein export (PubMed : 32344433). Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity (PubMed : 16891305, PubMed : 20940299). Involved in cellular calcium homeostasis regulation (PubMed : 22131369).

See full target information VAPB

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:112883 PubMed37498742

2023

COPI-regulated mitochondria-ER contact site formation maintains axonal integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel C Maddison,Bilal Malik,Leonardo Amadio,Dana M Bis-Brewer,Stephan Züchner,Owen M Peters,Gaynor A Smith

Science advances 6:eaax9093 PubMed32494695

2020

Pharmacological modulation of mitochondrial calcium uniporter controls lung inflammation in cystic fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandro Rimessi,Chiara Pozzato,Lorenzo Carparelli,Alice Rossi,Serena Ranucci,Ida De Fino,Cristina Cigana,Anna Talarico,Mariusz R Wieckowski,Carla M P Ribeiro,Claudio Trapella,Giacomo Rossi,Giulio Cabrini,Alessandra Bragonzi,Paolo Pinton

PloS one 14:e0214198 PubMed30921354

2019

Phenotypic subgrouping and multi-omics analyses reveal reduced diazepam-binding inhibitor (DBI) protein levels in autism spectrum disorder with severe language impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Chatravee Pichitpunpong,Surangrat Thongkorn,Songphon Kanlayaprasit,Wasana Yuwattana,Waluga Plaingam,Siriporn Sangsuthum,Wan Mohd Aizat,Syarul Nataqain Baharum,Tewin Tencomnao,Valerie Wailin Hu,Tewarit Sarachana

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VAPB antibody

Immunocytochemistry/ Immunofluorescence - Anti-VAPB antibody (AB196487)

Western blot - Anti-VAPB antibody (AB196487)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise