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Rabbit Recombinant Monoclonal VAPB antibody. Suitable for ICC/IF, WB, Dot and reacts with Human, Recombinant fragment - Human samples.

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Images

Western blot - Anti-VAPB antibody [EPR27026-59] (AB324047), expandable thumbnail
  • Western blot - Anti-VAPB antibody [EPR27026-59] (AB324047), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-VAPB antibody [EPR27026-59] (AB324047), expandable thumbnail
  • Dot Blot - Anti-VAPB antibody [EPR27026-59] (AB324047), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBDotIHC-P
Human
Tested
Tested
Expected
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Target data

Function

Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44 (PubMed:32344433, PubMed:33124732). Interacts with STARD3 in a FFAT motif phosphorylation dependent manner (PubMed:33124732). Via interaction with WDR44 participates in neosynthesized protein export (PubMed:32344433). Participates in the endoplasmic reticulum unfolded protein response (UPR) by inducing ERN1/IRE1 activity (PubMed:16891305, PubMed:20940299). Involved in cellular calcium homeostasis regulation (PubMed:22131369).

Alternative names

UNQ484/PRO983, VAPB, Vesicle-associated membrane protein-associated protein B/C, VAMP-B/VAMP-C, VAMP-associated protein B/C, VAP-B/VAP-C

Rabbit Recombinant Monoclonal VAPB antibody. Suitable for ICC/IF, WB, Dot and reacts with Human, Recombinant fragment - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27026-59
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-VAPB antibody [EPR27026-59] (ab324047), expandable thumbnail

    Western blot - Anti-VAPB antibody [EPR27026-59] (ab324047)

    VAPB Western blot staining using rabbit Anti-VAPB antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-VAPB antibody [EPR27026-59] (ab324047) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 2: HepG2 (human hepatocelullar carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: Human testis tisse lysate at 20 µg

    Lane 4: Human liver tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 27 kDa

    Exposure time: 26s

  • Western blot - Anti-VAPB antibody [EPR27026-59] (ab324047), expandable thumbnail

    Western blot - Anti-VAPB antibody [EPR27026-59] (ab324047)

    VAPB Western blot staining using rabbit Anti-VAPB antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-VAPB antibody [EPR27026-59] (ab324047) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HeLa transfected with siRNA specifically targeting VAPB whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 27 kDa, 36 kDa

    Exposure time: 26s

  • Immunocytochemistry/ Immunofluorescence - Anti-VAPB antibody [EPR27026-59] (ab324047), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-VAPB antibody [EPR27026-59] (ab324047)

    VAPB Immunocytochemistry/ Immunofluorescence staining of HeLa (human cervical adenocarcinoma epithelial cell) using rabbit Anti-VAPB antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling VAPB with ab324047 at 1/50 (9.76 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing cytoplasmic staining in HeLa cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Anti-KDEL mouse monoclonal antibody was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution (Magenta).

    -ve control 1: ab324047 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
    -ve control 2: Anti-KDEL mouse monoclonal antibody at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

  • Dot Blot - Anti-VAPB antibody [EPR27026-59] (ab324047), expandable thumbnail

    Dot Blot - Anti-VAPB antibody [EPR27026-59] (ab324047)

    VAPB Dot Blot staining using rabbit Anti-VAPB antibody

    Dot blot analysis of VAPB using ab324047 at 1:1000 (0.488 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Lane 1: His-tagged human VAPB recombinant protein

    Lane 2: His-tagged human VAPA recombinant protein

    Exposure time: 180 seconds.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human VAPA.

    In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Dot Blot - Anti-VAPB antibody [EPR27026-59] (ab324047) at 1/1000 dilution

    Lane 1: His-tagged human VAPB recombinant protein

    Lane 2: His-tagged human VAPA recombinant protein

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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