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AB212015

Anti-Varicella Zoster Virus IE62 antibody [62B]

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(2 Publications)

Mouse Monoclonal Varicella Zoster Virus IE62 antibody. Suitable for WB, ICC/IF and reacts with Varicella zoster virus samples. Cited in 2 publications. Immunogen corresponding to Virus preparation containing ORF62 protein.

View Alternative Names

ORF71, ORF62, Major viral transcription factor ICP4 homolog, Immediate-early protein 62, IE62, HHV3, Human Herpes virus 3, IE62, ORF 62, VZV, Varicella Zoster virus

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Varicella Zoster Virus IE62 antibody [62B] (AB212015)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Varicella Zoster Virus IE62 antibody [62B] (AB212015)

Immuofluorescent analysis of Varicella Zoster Virus (VZV) -infected MRC5 cells labeling Varicella Zoster Virus IE62 with ab212015 at 1/100 dilution, followed by Alexa Fluor 488 donkey anti-mouse IgG [H+L] at 1/200 dilution (green). Nuclei were stained with Hoechst 33342 (blue).

Western blot - Anti-Varicella Zoster Virus IE62 antibody [62B] (AB212015)
  • WB

Supplier Data

Western blot - Anti-Varicella Zoster Virus IE62 antibody [62B] (AB212015)

All lanes:

Western blot - Anti-Varicella Zoster Virus IE62 antibody [62B] (ab212015) at 1/5000 dilution

Lane 1:

VZV strain pOka infected MRC5 cell lysate

Lane 2:

MRC5 cell lysate (uninfected negative control)

Predicted band size: 140 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

62B

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Varicella zoster virus

Applications

ICC/IF, WB

applications

Immunogen

Virus preparation containing ORF62 protein. The exact immunogen used to generate this antibody is proprietary information.

Q8AZM1

Specificity

This antibody reacts with IE62 of VZV.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Varicella zoster virus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Proprietary technique
Purification notes
ab212015 was produced by hybridoma grown in serum-free medium and purified by a proprietary chromatography procedure under mild conditions. 90~95% pure by SDS-PAGE.
Storage buffer
pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional transactivator. May interact with and recruit specific components of the general transcription machinery to viral promoters and stabilize their formation for transcription initiation. Negatively regulates its own transcription. This immediate early (EI) protein may be necessary in virion for viral pathogenesis (By similarity).
See full target information ORF62
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Virology journal 21:253 PubMed39385182

2024

Upregulation of keratin 15 is required for varicella-zoster virus replication in keratinocytes and is attenuated in the live attenuated vOka vaccine strain.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Tommasi,Ohad Yogev,Michael B Yee,Andriani Drousioti,Meleri Jones,Alice Ring,Manuraj Singh,Inga Dry,Oscar Atkins,Aishath S Naeem,Nisha Kriplani,Arne N Akbar,Jürgen G Haas,Edel A O'Toole,Paul R Kinchington,Judith Breuer

eLife 9: PubMed32234209

2020

Functionally heterogeneous human satellite cells identified by single cell RNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Emilie Barruet,Steven M Garcia,Katharine Striedinger,Jake Wu,Solomon Lee,Lauren Byrnes,Alvin Wong,Sun Xuefeng,Stanley Tamaki,Andrew S Brack,Jason H Pomerantz
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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