AB108216

Anti-VARP antibody

Name: Anti-VARP antibody (ab108216) | Abcam
Brand: Abcam Limited
SKU: AB108216

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(4 Publications)

Rabbit Polyclonal VARP antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ANKRD27 aa 1000 to C-terminus.

View Alternative Names

PP12899, ANKRD27, Ankyrin repeat domain-containing protein 27, VPS9 domain-containing protein

2 Images

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Immunoprecipitation - Anti-VARP antibody (AB108216)

Detection of VARP by Western Blot of Immunprecipitate.
Lane 1 : ab108216 at 1µg/ml staining VARP in HeLa whole cell lysate immunoprecipitated using ab108216 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2 : Control IgG
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-VARP antibody (ab108216)

Predicted band size: 117 kDa

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Unknown

Western blot - Anti-VARP antibody (AB108216)

All lanes:

Western blot - Anti-VARP antibody (ab108216) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 117 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human ANKRD27 aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96NW4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab108216 was affinity purified using an epitope specific to VARP immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VARP also known as VPS9-ankyrin-repeat protein is a cytosolic protein with a molecular weight of about 135 kDa. It functions as a guanine nucleotide exchange factor (GEF) specifically for RAB21 a small GTPase involved in endosomal trafficking. VARP is expressed in various tissues with notable presence in the brain and immune cells. The protein contains several functional domains including ankyrin repeats and a VPS9 domain that facilitate its role in vesicular transport processes.

Biological function summary

The function of VARP extends beyond its GEF activity engaging in the regulation of endosomal trafficking and fusion events. It forms part of a complex with other multiprotein assemblies notably interacting with the retromer complex which is essential for the retrograde transport of endosomal cargo. VARP's interaction with endosomal membranes aids in the sorting and recycling of membrane proteins playing an integral role in maintaining cellular homeostasis.

Pathways

VARP contributes significantly to endocytic and recycling pathways which are pertinent for cell surface receptor distribution and nutrient uptake. The protein interacts with SNAREs such as VAMP7 facilitating vesicle fusion and trafficking. The importance of VARP in these pathways aligns with the function of the RAB GTPases family where it modulates the recycling and sorting routes critical for cellular logistics.

VARP is associated with neurodegenerative conditions and immunological disorders. The disruption of VARP function can lead to impaired endosomal trafficking contributing to diseases like Alzheimer's. It is connected to the pathology of the disease through its interaction with the retromer complex which is implicated in amyloid precursor protein processing. In addition anomalies in VARP expression or function can affect immune responses demonstrating links to immunodeficiencies through its interaction with RAB21.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

May be a guanine exchange factor (GEF) for Rab21, Rab32 and Rab38 and regulate endosome dynamics (PubMed : 16525121, PubMed : 18477474). May regulate the participation of VAMP7 in membrane fusion events; in vitro inhibits VAMP7-mediated SNARE complex formation by trapping VAMP7 in a closed, fusogenically inactive conformation (PubMed : 23104059). Involved in peripheral melanosomal distribution of TYRP1 in melanocytes; the function, which probably is implicating vesicle-trafficking, includes cooperation with Rab32, Rab38 and VAMP7 (By similarity). Involved in the regulation of neurite growth; the function seems to require its GEF activity, probably towards Rab21, and VAMP7 but not Rab32/38 (By similarity). Proposed to be involved in Golgi sorting of VAMP7 and transport of VAMP7 vesicles to the cell surface; the function seems to implicate kinesin heavy chain isoform 5 proteins, GOLGA4, RAB21 and MACF1 (PubMed : 22705394). Required for the colocalization of VAMP7 and Rab21, probably on TGN sites (PubMed : 19745841). Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with VPS29 (PubMed : 24856514). Regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (By similarity).

See full target information ANKRD27

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell 186:2219-2237.e29 PubMed37172566

2023

Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Michael D Healy,Kerrie E McNally,Rebeka Butkovič,Molly Chilton,Kohji Kato,Joanna Sacharz,Calum McConville,Edmund R R Moody,Shrestha Shaw,Vicente J Planelles-Herrero,Sathish K N Yadav,Jennifer Ross,Ufuk Borucu,Catherine S Palmer,Kai-En Chen,Tristan I Croll,Ryan J Hall,Nikeisha J Caruana,Rajesh Ghai,Thi H D Nguyen,Kate J Heesom,Shinji Saitoh,Imre Berger,Christiane Schaffitzel,Tom A Williams,David A Stroud,Emmanuel Derivery,Brett M Collins,Peter J Cullen

Nature communications 11:5031 PubMed33024112

2020

Mechanism and evolution of the Zn-fingernail required for interaction of VARP with VPS29.

Applications

Unspecified application

Species

Unspecified reactive species

Harriet Crawley-Snowdon,Ji-Chun Yang,Nathan R Zaccai,Luther J Davis,Lena Wartosch,Emily K Herman,Nicholas A Bright,James S Swarbrick,Brett M Collins,Lauren P Jackson,Matthew N J Seaman,J Paul Luzio,Joel B Dacks,David Neuhaus,David J Owen

Cell reports 31:107750 PubMed32521275

2020

TBC1D5-Catalyzed Cycling of Rab7 Is Required for Retromer-Mediated Human Papillomavirus Trafficking during Virus Entry.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Xie,Erin N Heim,Mac Crite,Daniel DiMaio

Journal of cell science 127:4940-53 PubMed25278552

2014

Identification of molecular heterogeneity in SNX27-retromer-mediated endosome-to-plasma-membrane recycling.

Applications

Unspecified application

Species

Unspecified reactive species

Ian J McGough,Florian Steinberg,Matthew Gallon,Ayaka Yatsu,Norihiko Ohbayashi,Kate J Heesom,Mitsunori Fukuda,Peter J Cullen

View all publications

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Product promise

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Anti-VARP antibody

Immunoprecipitation - Anti-VARP antibody (AB108216)

Western blot - Anti-VARP antibody (AB108216)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise