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AB317236

Anti-Vasa antibody [HL2485]

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Rabbit Monoclonal antibody. Suitable for WB, IHC - Wmt and reacts with Mouse, Rat, Zebrafish samples.
4 Images
Western blot - Anti-Vasa antibody [HL2485] (AB317236)
  • WB

Supplier Data

Western blot - Anti-Vasa antibody [HL2485] (AB317236)

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE and the membrane was blotted with ab317236 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Vasa antibody [HL2485] (ab317236) at 1/1000 dilution

Lane 1:

Mouse testis tissue extract at 50 µg

Lane 2:

Mouse spleen tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Vasa antibody [HL2485] (AB317236)
  • WB

Supplier Data

Western blot - Anti-Vasa antibody [HL2485] (AB317236)

Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE and the membrane was blotted with ab317236 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Vasa antibody [HL2485] (ab317236) at 1/1000 dilution

All lanes:

Rat testis tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

IHC - Wholemount - Anti-Vasa antibody [HL2485] (AB317236)
  • IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Vasa antibody [HL2485] (AB317236)

ab317236 detects Vasa protein on whole mount zebrafish by immunohistochemical analysis. Sample : Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo. Green : Vasa stained by ab317236 diluted at 1 : 100. Antigen Retrieval : Tris-HCl buffer pH 9.0 20 min at 70degC.

Western blot - Anti-Vasa antibody [HL2485] (AB317236)
  • WB

Supplier Data

Western blot - Anti-Vasa antibody [HL2485] (AB317236)

Zebrafish embryo extracts (30 ug) was separated by 7.5% SDS-PAGE and the membrane was blotted with ab317236 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Vasa antibody [HL2485] (ab317236) at 1/1000 dilution

All lanes:

3 dpf zebrafish embryo extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2485

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Zebrafish

Applications

WB, IHC - Wmt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCWmt-species-checked": "guaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCWmt-species-checked": "guaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/100 - 1/500", "IHCWmt-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VASA also known as DDX4 is a DEAD-box RNA helicase enzyme weighing approximately 79 kDa. VASA is expressed mainly in germ cells including those within the testes and ovaries. Mechanically it unwinds RNA secondary structures facilitating processes like translation and stabilization of RNA molecules. Its activity influences the process of germ cell differentiation by remodeling ribonucleoprotein complexes necessary for proper RNA metabolism.
Biological function summary

The protein plays an essential role in germ cell development and maintenance. VASA is part of the complex involved in ribonucleoprotein processing and it interacts closely with other proteins to regulate RNA dynamics in germ cells. It is fundamental for gametogenesis ensuring the production and maturation of both sperm and eggs. Research shows VASA activity directly affects meiotic chromosome segregation an important feature of reproductive biology.

Pathways

VASA integrates into the P granule pathways interacting with various components critical for germ line specification. It also intersects with the PIWI-interacting RNA (piRNA) pathways which prevent transposable element expression important for safeguarding genome stability. VASA's role alongside proteins like PIWI and AGO proteins illustrates its regulatory role in protecting germ cell genome integrity during development.

VASA's dysfunction relates strongly to infertility and germ cell tumors. Mutations or altered expression levels of VASA have implications in disorders such as spermatogenic failure and ovarian dysgenesis. Research also connects VASA with other proteins like DAZL and NANOS highlighting its involvement in maintaining germ cell homeostasis and avoidance of tumorigenic transformations.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

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Product promise

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For full details, please see our Terms & Conditions

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