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Rabbit Polyclonal VASA1 antibody. Suitable for WB, IHC - Wmt and reacts with Zebrafish samples. Cited in 13 publications.

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Images

Western blot - Anti-VASA/VAS antibody (AB209710), expandable thumbnail
  • IHC - Wholemount - Anti-VASA/VAS antibody (AB209710), expandable thumbnail
  • IHC - Wholemount - Anti-VASA/VAS antibody (AB209710), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC - Wmt
Zebrafish
Tested
Tested

Tested
Tested

Species

Zebrafish

Dilution info

1/500 - 1/3000

Notes

-

Tested
Tested

Species

Zebrafish

Dilution info

1/100 - 1/500

Notes

-

Recommended products

Rabbit Polyclonal VASA1 antibody. Suitable for WB, IHC - Wmt and reacts with Zebrafish samples. Cited in 13 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

VASA also known as DDX4 is a DEAD-box RNA helicase enzyme weighing approximately 79 kDa. VASA is expressed mainly in germ cells including those within the testes and ovaries. Mechanically it unwinds RNA secondary structures facilitating processes like translation and stabilization of RNA molecules. Its activity influences the process of germ cell differentiation by remodeling ribonucleoprotein complexes necessary for proper RNA metabolism.

Biological function summary

The protein plays an essential role in germ cell development and maintenance. VASA is part of the complex involved in ribonucleoprotein processing and it interacts closely with other proteins to regulate RNA dynamics in germ cells. It is fundamental for gametogenesis ensuring the production and maturation of both sperm and eggs. Research shows VASA activity directly affects meiotic chromosome segregation an important feature of reproductive biology.

Pathways

VASA integrates into the P granule pathways interacting with various components critical for germ line specification. It also intersects with the PIWI-interacting RNA (piRNA) pathways which prevent transposable element expression important for safeguarding genome stability. VASA's role alongside proteins like PIWI and AGO proteins illustrates its regulatory role in protecting germ cell genome integrity during development.

Associated diseases and disorders

VASA's dysfunction relates strongly to infertility and germ cell tumors. Mutations or altered expression levels of VASA have implications in disorders such as spermatogenic failure and ovarian dysgenesis. Research also connects VASA with other proteins like DAZL and NANOS highlighting its involvement in maintaining germ cell homeostasis and avoidance of tumorigenic transformations.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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3 product images

  • Western blot - Anti-VASA/VAS antibody (ab209710), expandable thumbnail

    Western blot - Anti-VASA/VAS antibody (ab209710)

    7.5 % SDS-PAGE

    All lanes: Western blot - Anti-VASA/VAS antibody (ab209710) at 1/2000 dilution

    All lanes: Zebrafish 10 hpf lysate/extract at 30 µg

    Predicted band size: 72 kDa

  • IHC - Wholemount - Anti-VASA/VAS antibody (ab209710), expandable thumbnail

    IHC - Wholemount - Anti-VASA/VAS antibody (ab209710)

    Immunohistochemical analysis (whole mount) of paraformaldehyde-fixed Zebrafish embryo labeling VASA/VAS (primordial germ cell) with ab209710 at 1/200 dilution.

  • IHC - Wholemount - Anti-VASA/VAS antibody (ab209710), expandable thumbnail

    IHC - Wholemount - Anti-VASA/VAS antibody (ab209710)

    Immunohistochemical analysis (whole mount) of Zebrafish 7 days-post-fertilization medaka embryo labeling VASA/VAS with ab209710 at 1/100 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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