AB209710

Anti-VASA/VAS antibody

Name: Anti-VASA/VAS antibody (ab209710) | Abcam
Brand: Abcam Limited
SKU: AB209710
Price: 510 USD
Availability: InStock

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(14 Publications)

Rabbit Polyclonal VASA1 antibody. Suitable for WB, IHC - Wmt and reacts with Zebrafish samples. Cited in 14 publications.

5 Images

IHC - Wholemount - Anti-VASA/VAS antibody (AB209710)

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-VASA/VAS antibody (AB209710)

Immunohistochemical analysis (whole mount) of Zebrafish 7 days-post-fertilization medaka embryo labeling VASA/VAS with ab209710 at 1/100 dilution.

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-VASA/VAS antibody (AB209710)

Immunohistochemical analysis (whole mount) of paraformaldehyde-fixed Zebrafish embryo labeling VASA/VAS (primordial germ cell) with ab209710 at 1/200 dilution.

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-VASA/VAS antibody (AB209710)

Vasa antibody detects Vasa protein on whole mount zebrafish by immunohistochemical analysis.
Sample : Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.
Green : Vasa stained by Vasa antibody diluted at 1/100 dilution.
Antigen Retrieval : Tris-HCl buffer, pH 9.0, 20 min at 70°C

Supplier Data

Western blot - Anti-VASA/VAS antibody (AB209710)

7.5 % SDS-PAGE

All lanes:

Western blot - Anti-VASA/VAS antibody (ab209710) at 1/2000 dilution

All lanes:

Zebrafish 10 hpf lysate/extract at 30 µg

Predicted band size: 72 kDa

false

Supplier Data

Western blot - Anti-VASA/VAS antibody (AB209710)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-VASA/VAS antibody (ab209710) at 1/2000 dilution

All lanes:

Whole zebrafish extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 77 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Zebrafish

Applications

IHC - Wmt, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/100 - 1/500", "IHCWmt-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VASA also known as DDX4 is a DEAD-box RNA helicase enzyme weighing approximately 79 kDa. VASA is expressed mainly in germ cells including those within the testes and ovaries. Mechanically it unwinds RNA secondary structures facilitating processes like translation and stabilization of RNA molecules. Its activity influences the process of germ cell differentiation by remodeling ribonucleoprotein complexes necessary for proper RNA metabolism.

Biological function summary

The protein plays an essential role in germ cell development and maintenance. VASA is part of the complex involved in ribonucleoprotein processing and it interacts closely with other proteins to regulate RNA dynamics in germ cells. It is fundamental for gametogenesis ensuring the production and maturation of both sperm and eggs. Research shows VASA activity directly affects meiotic chromosome segregation an important feature of reproductive biology.

Pathways

VASA integrates into the P granule pathways interacting with various components critical for germ line specification. It also intersects with the PIWI-interacting RNA (piRNA) pathways which prevent transposable element expression important for safeguarding genome stability. VASA's role alongside proteins like PIWI and AGO proteins illustrates its regulatory role in protecting germ cell genome integrity during development.

VASA's dysfunction relates strongly to infertility and germ cell tumors. Mutations or altered expression levels of VASA have implications in disorders such as spermatogenic failure and ovarian dysgenesis. Research also connects VASA with other proteins like DAZL and NANOS highlighting its involvement in maintaining germ cell homeostasis and avoidance of tumorigenic transformations.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information VASA/VAS

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Fish physiology and biochemistry 49:1277-1294 PubMed37878190

2023

Production of sterile mono-sex triploid yellow drum (Nibea albiflora): genotypic females and sex-reversed phenotypic males with emphasis on utilization as surrogate broodstock.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Lei Lu,Ruiyi Chen,Liechao Yu,Weihua Hu,Dongdong Xu

Animal bioscience 37:50-60 PubMed37641828

2023

Testicular fat deposition attenuates reproductive performance via decreased follicle-stimulating hormone level and sperm meiosis and testosterone synthesis in mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Du,Shikun Chen,Yang Chen,Xinxu Yuan,Huansheng Dong

Cells 12: PubMed36831339

2023

Two Novel lncRNAs Regulate Primordial Germ Cell Development in Zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Li,Wei Liu,Chengyu Mo,Meisheng Yi,Jianfang Gui

Antioxidants (Basel, Switzerland) 11: PubMed36009286

2022

Zinc Oxide Nanoparticles (ZnO-NPs) Suppress Fertility by Activating Autophagy, Apoptosis, and Oxidative Stress in the Developing Oocytes of Female Zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Suzan Attia Mawed,Carlotta Marini,Mahmoud Alagawany,Mayada R Farag,Rasha M Reda,Mohamed T El-Saadony,Walaa M Elhady,Gian E Magi,Alessandro Di Cerbo,Wafaa G El-Nagar

Zoological research 43:537-551 PubMed35616259

2022

Production of functional sperm from -cultured premeiotic spermatogonia in a marine fish.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Zhang,Wan-Wan Zhang,Cheng-Yu Mo,Meng-Dan Dong,Kun-Tong Jia,Wei Liu,Mei-Sheng Yi

Acta histochemica 124:151870 PubMed35218995

2022

Immunolocalization of Vasa, PIWI, and TDRKH proteins in male germ cells during spermatogenesis of the teleost fish Poecilia reticulata.

Applications

Unspecified application

Species

Unspecified reactive species

L Milani,F Cinelli,M Iannello,M Lazzari,V Franceschini,M G Maurizii

PLoS genetics 17:e1009667 PubMed34214072

2021

The RNA-binding protein Igf2bp3 is critical for embryonic and germline development in zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Ho Vong,Lavanya Sivashanmugam,Rebecca Leech,Andreas Zaucker,Alex Jones,Karuna Sampath

Histochemistry and cell biology 156:19-34 PubMed33770286

2021

Early germline differentiation in bivalves: TDRD7 as a candidate investigational unit for Ruditapes philippinarum germ granule assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Beatrice Filanti,Giovanni Piccinini,Simone Bettini,Maurizio Lazzari,Valeria Franceschini,Maria Gabriella Maurizii,Liliana Milani

eLife 10: PubMed33646121

2021

Cystic proliferation of germline stem cells is necessary to reproductive success and normal mating behavior in medaka.

Applications

Unspecified application

Species

Unspecified reactive species

Luisa F Arias Padilla,Diana C Castañeda-Cortés,Ivana F Rosa,Omar D Moreno Acosta,Ricardo S Hattori,Rafael H Nóbrega,Juan I Fernandino

World journal of stem cells 12:500-513 PubMed32742567

2020

Cytotoxicity of nonylphenol on spermatogonial stem cells phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Hao Lei,Wen Yan,Chun-Hua Luo,Yu-Ming Guo,Yang-Yang Zhang,Xing-Huan Wang,Xin-Jun Su

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-VASA/VAS antibody

IHC - Wholemount - Anti-VASA/VAS antibody (AB209710)

IHC - Wholemount - Anti-VASA/VAS antibody (AB209710)

IHC - Wholemount - Anti-VASA/VAS antibody (AB209710)

Western blot - Anti-VASA/VAS antibody (AB209710)

Western blot - Anti-VASA/VAS antibody (AB209710)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise