Rabbit Polyclonal VASA1 antibody. Suitable for WB, IHC - Wmt and reacts with Zebrafish samples. Cited in 13 publications.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
WB | IHC - Wmt | |
---|---|---|
Zebrafish | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Zebrafish | Dilution info 1/500 - 1/3000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Zebrafish | Dilution info 1/100 - 1/500 | Notes - |
Rabbit Polyclonal VASA1 antibody. Suitable for WB, IHC - Wmt and reacts with Zebrafish samples. Cited in 13 publications.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
VASA also known as DDX4 is a DEAD-box RNA helicase enzyme weighing approximately 79 kDa. VASA is expressed mainly in germ cells including those within the testes and ovaries. Mechanically it unwinds RNA secondary structures facilitating processes like translation and stabilization of RNA molecules. Its activity influences the process of germ cell differentiation by remodeling ribonucleoprotein complexes necessary for proper RNA metabolism.
The protein plays an essential role in germ cell development and maintenance. VASA is part of the complex involved in ribonucleoprotein processing and it interacts closely with other proteins to regulate RNA dynamics in germ cells. It is fundamental for gametogenesis ensuring the production and maturation of both sperm and eggs. Research shows VASA activity directly affects meiotic chromosome segregation an important feature of reproductive biology.
VASA integrates into the P granule pathways interacting with various components critical for germ line specification. It also intersects with the PIWI-interacting RNA (piRNA) pathways which prevent transposable element expression important for safeguarding genome stability. VASA's role alongside proteins like PIWI and AGO proteins illustrates its regulatory role in protecting germ cell genome integrity during development.
VASA's dysfunction relates strongly to infertility and germ cell tumors. Mutations or altered expression levels of VASA have implications in disorders such as spermatogenic failure and ovarian dysgenesis. Research also connects VASA with other proteins like DAZL and NANOS highlighting its involvement in maintaining germ cell homeostasis and avoidance of tumorigenic transformations.
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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7.5 % SDS-PAGE
All lanes: Western blot - Anti-VASA/VAS antibody (ab209710) at 1/2000 dilution
All lanes: Zebrafish 10 hpf lysate/extract at 30 µg
Predicted band size: 72 kDa
Immunohistochemical analysis (whole mount) of paraformaldehyde-fixed Zebrafish embryo labeling VASA/VAS (primordial germ cell) with ab209710 at 1/200 dilution.
Immunohistochemical analysis (whole mount) of Zebrafish 7 days-post-fertilization medaka embryo labeling VASA/VAS with ab209710 at 1/100 dilution.
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