Anti-Vasopressin antibody [EPR20602]

7 product images

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  Western blot - Anti-Vasopressin antibody [EPR20602] (ab213708)

  Blocking/Dilution buffer: 5% NFDM/TBST.

  All lanes: Western blot - Anti-Vasopressin antibody [EPR20602] (ab213708) at 1/2000 dilution

  Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 10 µg

  Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Developed using the ECL technique.

  Predicted band size: 17 kDa

  Exposure time: 1s

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)

  Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling Vasopressin with ab213708 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neurons of mouse paraventricular nucleus (PMID: 22822466, PMID: 17437647, PMID: 18773953). Counter stained with hematoxylin.
  

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)

  Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labeling Vasopressin with ab213708 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neurons of rat paraventricular nucleus (PMID: 22822466, PMID: 17437647, PMID: 18773953). Counter stained with hematoxylin.
  

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)

  Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain tissue labeling Vasopressin with ab213708 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the neurons of paraventricular nucleus on mouse brain tissue section is observed.
  

  The nuclear counter stain is DAPI (blue).

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

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  Immunoprecipitation - Anti-Vasopressin antibody [EPR20602] (ab213708)

  Vasopressin was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Vasopressin expression vector containing a myc-His-tag^®, whole cell lysate with ab213708 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab213708 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution
  

  Lane 1: HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag^®, whole cell lysate 10 μg (Input).

  Lane 2: ab213708 IP in HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag^®, whole cell lysate.

  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab213708 in HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag^®, whole cell lysate.

  Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  Exposure time: 10 seconds.

  All lanes: Immunoprecipitation - Anti-Vasopressin antibody [EPR20602] (ab213708)

  Predicted band size: 17 kDa

• 

  Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] (ab213708)

  Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat brain tissue labeling Vasopressin with ab213708 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the neurons of paraventricular nucleus on rat brain tissue section is observed.
  

  The nuclear counter stain is DAPI (blue).

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

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  Flow Cytometry (Intracellular) - Anti-Vasopressin antibody [EPR20602] (ab213708)

  Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected withVasopressin expression vector containing a myc-His-tag^® cell line labeling Vasopressin with ab213708 at 1/500 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
  

  Co-stained with Alexa Fluor^® 647 conjugated Myc-tag (Y-axis). Only Myc (+) population showed Vasopressin positive staining.