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Rabbit Recombinant Monoclonal Vasopressin antibody. Carrier free. Suitable for IP, WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Transfected cell lysate - Human, Rat, Mouse, Human, Transfected cell line - Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (AB227050), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (AB227050), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (AB227050), expandable thumbnail
  • Immunoprecipitation - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (AB227050), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (AB227050), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-FrFlow Cyt (Intra)IHC-P
Human
Expected
Expected
Expected
Expected
Expected
Mouse
Expected
Expected
Tested
Expected
Tested
Rat
Expected
Expected
Tested
Expected
Tested
Transfected cell line - Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Transfected cell lysate - Human
Tested
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Transfected cell lysate - Human

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

-

Notes

-

Species

Mouse

Dilution info

-

Notes

-

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Transfected cell line - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Transfected cell lysate - Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell line - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

-

Notes

Perform heat mediated antigen retrieval by using sodium citrate buffer (pH6.0).

Species

Mouse

Dilution info

-

Notes

Perform heat mediated antigen retrieval by using sodium citrate buffer (pH6.0).

Expected
Expected

Species

Human

Dilution info

-

Notes

Perform heat mediated antigen retrieval by using sodium citrate buffer (pH6.0).

Not recommended
Not recommended

Species

Transfected cell lysate - Human, Transfected cell line - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Transfected cell line - Human

Dilution info

-

Notes

-

Expected
Expected

Species

Rat, Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Transfected cell lysate - Human, Transfected cell line - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

8 products for Alternative Version

Target data

Function

Neurophysin 2 specifically binds vasopressin.Vasopressin has a direct antidiuretic action on the kidney, it also causes vasoconstriction of the peripheral vessels. Acts by binding to vasopressin receptors (V1bR/AVPR1B, V1aR/AVPR1A, and V2R/AVPR2) (By similarity).

Alternative names

Vasopressin-neurophysin 2-copeptin, AVP-NPII, Avp

Recommended products

Rabbit Recombinant Monoclonal Vasopressin antibody. Carrier free. Suitable for IP, WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Transfected cell lysate - Human, Rat, Mouse, Human, Transfected cell line - Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR20602

Purification technique

Affinity purification Protein A

Specificity

In WB, FC and IP we detected Vasopressin only in overexpressing cells. Not recommended for human IHC-P as there are no suitable positive human tissues.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab227050 is the carrier-free version of Anti-Vasopressin antibody [EPR20602] ab213708.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050)

    Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling Vasopressin with Anti-Vasopressin antibody [EPR20602] ab213708 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neurons of mouse paraventricular nucleus (PMID: 22822466, PMID: 17437647, PMID: 18773953). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Vasopressin antibody [EPR20602] ab213708).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050)

    Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labeling Vasopressin with Anti-Vasopressin antibody [EPR20602] ab213708 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on neurons of rat paraventricular nucleus (PMID: 22822466, PMID: 17437647, PMID: 18773953). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Vasopressin antibody [EPR20602] ab213708).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain tissue labeling Vasopressin with Anti-Vasopressin antibody [EPR20602] ab213708  at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the neurons of paraventricular nucleus on mouse brain tissue section is observed.

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Vasopressin antibody [EPR20602] ab213708).

  • Immunoprecipitation - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050), expandable thumbnail

    Immunoprecipitation - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050)

    Vasopressin was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate with Anti-Vasopressin antibody [EPR20602] ab213708 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Vasopressin antibody [EPR20602] ab213708 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate 10 μg (Input).

    Lane 2: Anti-Vasopressin antibody [EPR20602] ab213708 IP in HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Vasopressin antibody [EPR20602] ab213708 in HEK-293T transfected with Vasopressin expression vector containing a myc-His-tag®, whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Vasopressin antibody [EPR20602] ab213708).

    All lanes: Immunoprecipitation - Anti-Vasopressin antibody [EPR20602] (Anti-Vasopressin antibody [EPR20602] ab213708)

    Predicted band size: 17 kDa

  • Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat brain tissue labeling Vasopressin with Anti-Vasopressin antibody [EPR20602] ab213708  at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Positive cytoplasmic staining in the neurons of paraventricular nucleus on rat brain tissue section is observed.

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Vasopressin antibody [EPR20602] ab213708).

  • Flow Cytometry (Intracellular) - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Vasopressin antibody [EPR20602] - BSA and Azide free (ab227050)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Vasopressin expression vector containing a myc-His-tag® cell line labeling Vasopressin with Anti-Vasopressin antibody [EPR20602] ab213708 at 1/500 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    Co-stained with Alexa Fluor® 647 conjugated Myc-tag (Y-axis). Only Myc (+) population showed Vasopressin positive staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Vasopressin antibody [EPR20602] ab213708).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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