AB109321

Anti-VASP antibody [EPR1337(2)]

20ul selling size
RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal VASP antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Vasodilator-stimulated phosphoprotein, VASP

6 Images

Lab

Immunoprecipitation - Anti-VASP antibody [EPR1337(2)] (AB109321)

ab109321 (purified) at 1/30 immunoprecipitating VASP in HEK293 cell lysate (Lane 1). Lane 2 - rabbit monoclonal IgG instead of ab109321 in HEK293 lysates. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-VASP antibody [EPR1337(2)] (ab109321)

Predicted band size: 39 kDa

Observed band size: 46 kDa

false

Lab

Western blot - Anti-VASP antibody [EPR1337(2)] (AB109321)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-VASP antibody [EPR1337(2)] (ab109321) at 1/5000 dilution

All lanes:

THP-1 cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

Predicted band size: 39 kDa,60 kDa,61 kDa,79 kDa

Observed band size: 46 kDa,60 kDa,79 kDa

false

Lab

Western blot - Anti-VASP antibody [EPR1337(2)] (AB109321)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-VASP antibody [EPR1337(2)] (ab109321) at 1/1000 dilution

All lanes:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

Predicted band size: 39 kDa

Observed band size: 46 kDa

false

Unknown

Western blot - Anti-VASP antibody [EPR1337(2)] (AB109321)

Lanes 1-4 : Merged signal (red and green). Green - ab109321 observed at 46 kDa. Red - loading control ab8245 observed at 36 kDa.

ab109321 Anti-VASP antibody [EPR1337(2)] was shown to specifically react with VASP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265892 (knockout cell lysate ab257792) was used. Wild-type and VASP knockout samples were subjected to SDS-PAGE. ab109321 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-VASP antibody [EPR1337(2)] (ab109321) at 1/1000 dilution

Lane 1:

Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

VASP knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

Western blot - Human VASP knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-vasp-knockout-hela-cell-line-ab265892'>ab265892</a>)

Lane 3:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 39 kDa

Observed band size: 46 kDa

false

Unknown

Western blot - Anti-VASP antibody [EPR1337(2)] (AB109321)

All lanes:

Western blot - Anti-VASP antibody [EPR1337(2)] (ab109321) at 1/10000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Human platelet lysate at 10 µg

Predicted band size: 39 kDa

Observed band size: 46 kDa

false

Lab

Western blot - Anti-VASP antibody [EPR1337(2)] (AB109321)

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Western blot - Anti-VASP antibody [EPR1337(2)] (ab109321) at 1/1000 dilution

All lanes:

HEK293 cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

Predicted band size: 39 kDa,85 kDa

Observed band size: 100 kDa,46 kDa

false

Carrier free

Anti-VASP antibody [EPR1337(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1337(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/70", "IP-species-notes": "For unpurified use at 1/10 - 1/100.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/10000.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VASP also known as Vasodilator-Stimulated Phosphoprotein is a 46-50 kDa protein involved in actin filament assembly and elongation. It plays an important role in cellular motility and the maintenance of cell shape. VASP is expressed in many cell types but is notably present in platelets and endothelial cells. It is an important player in signal transduction pathways that lead to cytoskeletal reorganization influencing cell movement and adhesion dynamics.

Biological function summary

VASP is an important component in cell movement regulation. It acts within a larger complex of actin-binding proteins that control the assembly of actin filaments. This protein influences cell adhesion migration and signal transduction processes. Through these actions VASP influences cellular responses to environmental cues making it essential for cellular dynamics and integrity during development and wound healing.

Pathways

VASP connects to significant signaling pathways such as the PI3K/Akt pathway and the cAMP pathway. It often interacts with proteins like c-Abl tyrosine kinase and Mena contributing to actin dynamics regulation. These pathways are critical for transducing signals from extracellular environments to intracellular responses allowing for coordinated cellular functions in processes such as cell migration and growth.

VASP plays a role in cardiovascular disease and cancer-related metastasis due to its function in cell motility and adhesion. Abnormal VASP activity is associated with impaired endothelial cell function and contributes to atherosclerosis development. Additionally it interacts with proteins like Mena and fascin in cancer cells aiding in metastatic progression by enhancing motility and invasive capabilities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.

See full target information VASP

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:372 PubMed40781226

2025

Activation of APOBEC3 cytidine deaminases and endogenous retroviruses is integrated by MUC1-C in NSCLC cells.

Applications

Unspecified application

Species

Unspecified reactive species

Naoki Haratake,Shinkichi Takamori,Hideko Isozaki,Keisuke Shigeta,Chie Kikutake,Hiroki Ozawa,Atrayee Bhattacharya,Ayako Nakashoji,Mikita Suyama,Tomoyoshi Takenaka,Tomoharu Yoshizumi,Atsushi Osoegawa,Aaron N Hata,Donald Kufe

Heliyon 10:e37110 PubMed39296110

2024

Anti-vasodilator-stimulated phosphoprotein (VASP) antibodies are associated with neuropsychiatric disorders in systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxi Zhu,Yan Liu,Jiayi Xu,Hang Yang,Yi Zhao,Yi Liu

ACS pharmacology & translational science 4:1567-1577 PubMed34661075

2021

Inhibition of Arginine Methylation Impairs Platelet Function.

Applications

Unspecified application

Species

Unspecified reactive species

Alistair James Marsden,David R J Riley,Antonia Barry,Jawad S Khalil,Barbara-Ann Guinn,Neil T Kemp,Francisco Rivero,Pedro Beltran-Alvarez

Microcirculation (New York, N.Y. : 1994) 27:e12608 PubMed31991513

2020

High-dose nitroglycerin administered during rewarming preserves erythrocyte deformability in cardiac surgery with cardiopulmonary bypass.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Hsuan Tai,You-Hsiang Chu,Hsiang-Ling Wu,Su-Man Lin,Mei-Yung Tsou,Cheng-Hsiung Huang,Hsiao-Huang Chang,Chih-Cherng Lu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VASP antibody [EPR1337(2)]

Immunoprecipitation - Anti-VASP antibody [EPR1337(2)] (AB109321)

Western blot - Anti-VASP antibody [EPR1337(2)] (AB109321)

Western blot - Anti-VASP antibody [EPR1337(2)] (AB109321)

Western blot - Anti-VASP antibody [EPR1337(2)] (AB109321)

Western blot - Anti-VASP antibody [EPR1337(2)] (AB109321)

Western blot - Anti-VASP antibody [EPR1337(2)] (AB109321)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise