AB114029

Anti-VASP antibody [OTI1H8]

Name: Anti-VASP antibody [OTI1H8] (ab114029) | Abcam
Brand: Abcam Limited
SKU: AB114029
Price: 495 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal VASP antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, African green monkey samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human VASP.

View Alternative Names

Vasodilator-stimulated phosphoprotein, VASP

4 Images

Flow Cytometry (Intracellular) - Anti-VASP antibody [OTI1H8] (AB114029)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-VASP antibody [OTI1H8] (AB114029)

ab114029 at 1/100 dilution staining VASP in HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY VASP overexpress plasmid (Red) or empty vector control plasmid (Blue) by Flow Cytometry (Intracellular).

Supplier Data

Western blot - Anti-VASP antibody [OTI1H8] (AB114029)

All lanes:

Western blot - Anti-VASP antibody [OTI1H8] (ab114029) at 1/500 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cell lysate transfected with pCMV6-ENTRY VASP cDNA at 5 µg

Predicted band size: 39 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-VASP antibody [OTI1H8] (AB114029)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VASP antibody [OTI1H8] (AB114029)

ab114029 at 1/100 dilution staining VASP in COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY VASP by Immunofluorescence.

Supplier Data

Western blot - Anti-VASP antibody [OTI1H8] (AB114029)

All lanes:

Western blot - Anti-VASP antibody [OTI1H8] (ab114029) at 1/500 dilution

Lane 1:

HepG2 (Human liver hepatocellular carcinoma cell line) cell extract at 35 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg

Lane 3:

HT-29 (Human colorectal adenocarcinoma cell line) cell extract at 35 µg

Lane 4:

A549 (Human lung carcinoma cell line) cell extract at 35 µg

Lane 5:

COS-7 (African green monkey kidney fibroblast-like cell line) cell extract at 35 µg

Lane 6:

Jurkat (Human T cell leukemia cell line from peripheral blood) cell extract at 35 µg

Lane 7:

MDCK (Canine kidney cell line) cell extract at 35 µg

Lane 8:

PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract at 35 µg

Lane 9:

MCF7 (Human breast adenocarcinoma cell line) cell extract at 35 µg

Predicted band size: 39 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1H8

Isotype

IgG1

Carrier free

Reacts with

Human, African green monkey

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

Recombinant Full Length Protein corresponding to Human VASP.

P50552

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "African green monkey": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

The clone number has been updated from 1H8 to OTI1H8, both clone numbers name the same clone.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from cell culture supernatant by affinity chromatography

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VASP also known as Vasodilator-Stimulated Phosphoprotein is a 46-50 kDa protein involved in actin filament assembly and elongation. It plays an important role in cellular motility and the maintenance of cell shape. VASP is expressed in many cell types but is notably present in platelets and endothelial cells. It is an important player in signal transduction pathways that lead to cytoskeletal reorganization influencing cell movement and adhesion dynamics.

Biological function summary

VASP is an important component in cell movement regulation. It acts within a larger complex of actin-binding proteins that control the assembly of actin filaments. This protein influences cell adhesion migration and signal transduction processes. Through these actions VASP influences cellular responses to environmental cues making it essential for cellular dynamics and integrity during development and wound healing.

Pathways

VASP connects to significant signaling pathways such as the PI3K/Akt pathway and the cAMP pathway. It often interacts with proteins like c-Abl tyrosine kinase and Mena contributing to actin dynamics regulation. These pathways are critical for transducing signals from extracellular environments to intracellular responses allowing for coordinated cellular functions in processes such as cell migration and growth.

VASP plays a role in cardiovascular disease and cancer-related metastasis due to its function in cell motility and adhesion. Abnormal VASP activity is associated with impaired endothelial cell function and contributes to atherosclerosis development. Additionally it interacts with proteins like Mena and fascin in cancer cells aiding in metastatic progression by enhancing motility and invasive capabilities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.

See full target information VASP

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 14:e0218971 PubMed31269038

2019

Cadherin-11 contributes to liver fibrosis induced by carbon tetrachloride.

Applications

Unspecified application

Species

Unspecified reactive species

Mesias Pedroza,Sarah To,Jennifer Smith,Sandeep K Agarwal

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VASP antibody [OTI1H8]

Flow Cytometry (Intracellular) - Anti-VASP antibody [OTI1H8] (AB114029)

Western blot - Anti-VASP antibody [OTI1H8] (AB114029)

Immunocytochemistry/ Immunofluorescence - Anti-VASP antibody [OTI1H8] (AB114029)

Western blot - Anti-VASP antibody [OTI1H8] (AB114029)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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