AB58555

Anti-VASP (phospho S157) antibody [5C6]

Name: Anti-VASP (phospho S157) antibody [5C6] (ab58555) | Abcam
Brand: Abcam Limited
SKU: AB58555
Price: 470 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(7 Publications)

Mouse Monoclonal VASP phospho S157 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human Vasodilator-stimulated phosphoprotein phospho S157 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Vasodilator-stimulated phosphoprotein, VASP

1 Images

Flow Cytometry - Anti-VASP (phospho S157) antibody [5C6] (AB58555)

Flow Cyt

Unknown

Flow Cytometry - Anti-VASP (phospho S157) antibody [5C6] (AB58555)

Overlay histogram showing THP1 cells stained with ab58555 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58555, 0.5μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5C6

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Synthetic Peptide within Human Vasodilator-stimulated phosphoprotein phospho S157 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P50552

Specificity

This antibody recognizes the the ~50 kDa VASP protein phosphorylated at Ser¹⁵⁷. It does not cross-react with non-phosphorylated VASP or with un-related phosphorylation sites.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.5 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography.

Storage buffer

Preservative: 0.1% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VASP also known as Vasodilator-Stimulated Phosphoprotein is a 46-50 kDa protein involved in actin filament assembly and elongation. It plays an important role in cellular motility and the maintenance of cell shape. VASP is expressed in many cell types but is notably present in platelets and endothelial cells. It is an important player in signal transduction pathways that lead to cytoskeletal reorganization influencing cell movement and adhesion dynamics.

Biological function summary

VASP is an important component in cell movement regulation. It acts within a larger complex of actin-binding proteins that control the assembly of actin filaments. This protein influences cell adhesion migration and signal transduction processes. Through these actions VASP influences cellular responses to environmental cues making it essential for cellular dynamics and integrity during development and wound healing.

Pathways

VASP connects to significant signaling pathways such as the PI3K/Akt pathway and the cAMP pathway. It often interacts with proteins like c-Abl tyrosine kinase and Mena contributing to actin dynamics regulation. These pathways are critical for transducing signals from extracellular environments to intracellular responses allowing for coordinated cellular functions in processes such as cell migration and growth.

VASP plays a role in cardiovascular disease and cancer-related metastasis due to its function in cell motility and adhesion. Abnormal VASP activity is associated with impaired endothelial cell function and contributes to atherosclerosis development. Additionally it interacts with proteins like Mena and fascin in cancer cells aiding in metastatic progression by enhancing motility and invasive capabilities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.

See full target information Vasodilator-stimulated phosphoprotein phospho S157

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell chemical biology 26:1393-1406.e7 PubMed31353320

2019

Compartmentalized cAMP Generation by Engineered Photoactivated Adenylyl Cyclases.

Applications

Unspecified application

Species

Unspecified reactive species

Colin P O'Banion,Brianna M Vickerman,Lauren Haar,David S Lawrence

Methods in enzymology 621:171-190 PubMed31128778

2019

Design, construction, and validation of optogenetic proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Colin P O'Banion,Anwesha Goswami,David S Lawrence

Experimental & molecular medicine 50:115 PubMed30185781

2018

Downregulation of the long noncoding RNA MBNL1-AS1 protects sevoflurane-pretreated mice against ischemia-reperfusion injury by targeting KCNMA1.

Applications

Unspecified application

Species

Unspecified reactive species

Xue-Feng Li,Zong-Qiang Wang,Long-Yun Li,Guo-Qing Zhao,Shao-Nan Yu

Cell chemical biology 25:100-109.e8 PubMed29104065

2017

Design and Profiling of a Subcellular Targeted Optogenetic cAMP-Dependent Protein Kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Colin P O'Banion,Melanie A Priestman,Robert M Hughes,Laura E Herring,Stephen J Capuzzi,David S Lawrence

The Journal of biological chemistry 288:16403-15 PubMed23615913

2013

Phosphorylation of serine 1137/1138 of mouse insulin receptor substrate (IRS) 2 regulates cAMP-dependent binding to 14-3-3 proteins and IRS2 protein degradation.

Applications

Unspecified application

Species

Human

Sabine S Neukamm,Jennifer Ott,Sascha Dammeier,Rainer Lehmann,Hans-Ulrich Häring,Erwin Schleicher,Cora Weigert

Neoplasia (New York, N.Y.) 14:1057-66 PubMed23226099

2012

Spontaneous cell fusion of acute leukemia cells and macrophages observed in cells with leukemic potential.

Applications

Unspecified application

Species

Unspecified reactive species

Ines Martin-Padura,Paola Marighetti,Giuliana Gregato,Alice Agliano,Omar Malazzi,Patrizia Mancuso,Giancarlo Pruneri,Andrea Viale,Francesco Bertolini

The Journal of biological chemistry 287:31393-405 PubMed22778267

2012

Zyxin is a transforming growth factor-β (TGF-β)/Smad3 target gene that regulates lung cancer cell motility via integrin α5β1.

Applications

Species

Unspecified reactive species

Nikica Mise,Rajkumar Savai,Haiying Yu,Johannes Schwarz,Naftali Kaminski,Oliver Eickelberg

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VASP (phospho S157) antibody [5C6]

Flow Cytometry - Anti-VASP (phospho S157) antibody [5C6] (AB58555)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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