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AB47268

Anti-VASP (phospho S157) antibody

4

(2 Reviews)

|

(5 Publications)

Rabbit Polyclonal VASP phospho S157 antibody. Suitable for ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human Vasodilator-stimulated phosphoprotein phospho S157 aa 100-200.

View Alternative Names

Vasodilator-stimulated phosphoprotein, VASP

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VASP (phospho S157) antibody (AB47268)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VASP (phospho S157) antibody (AB47268)

ab47268, at 1/50 dilution, staining VASP in Human tonsil tissue by Immunohistochemistry, Paraffin-embedded tissue. Left panel : no peptide; right panel : with immunizing phosphopeptide.

Western blot - Anti-VASP (phospho S157) antibody (AB47268)
  • WB

Unknown

Western blot - Anti-VASP (phospho S157) antibody (AB47268)

All lanes:

Western blot - Anti-VASP (phospho S157) antibody (ab47268)

Lane 1:

NIH/3T3 cell extract treated with forskolin (40µM, 30 min)

Lane 2:

NIH/3T3 cell extract

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, ELISA, WB

applications

Immunogen

Synthetic Peptide within Human Vasodilator-stimulated phosphoprotein phospho S157 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

P50552

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorlyation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VASP also known as Vasodilator-Stimulated Phosphoprotein is a 46-50 kDa protein involved in actin filament assembly and elongation. It plays an important role in cellular motility and the maintenance of cell shape. VASP is expressed in many cell types but is notably present in platelets and endothelial cells. It is an important player in signal transduction pathways that lead to cytoskeletal reorganization influencing cell movement and adhesion dynamics.
Biological function summary

VASP is an important component in cell movement regulation. It acts within a larger complex of actin-binding proteins that control the assembly of actin filaments. This protein influences cell adhesion migration and signal transduction processes. Through these actions VASP influences cellular responses to environmental cues making it essential for cellular dynamics and integrity during development and wound healing.

Pathways

VASP connects to significant signaling pathways such as the PI3K/Akt pathway and the cAMP pathway. It often interacts with proteins like c-Abl tyrosine kinase and Mena contributing to actin dynamics regulation. These pathways are critical for transducing signals from extracellular environments to intracellular responses allowing for coordinated cellular functions in processes such as cell migration and growth.

VASP plays a role in cardiovascular disease and cancer-related metastasis due to its function in cell motility and adhesion. Abnormal VASP activity is associated with impaired endothelial cell function and contributes to atherosclerosis development. Additionally it interacts with proteins like Mena and fascin in cancer cells aiding in metastatic progression by enhancing motility and invasive capabilities.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
See full target information Vasodilator-stimulated phosphoprotein phospho S157
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 135: PubMed40762838

2025

The protein deacetylase SIRT2 exerts metabolic control over adaptive β cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew Wortham,Bastian Ramms,Chun Zeng,Jacqueline R Benthuysen,Somesh Sai,Dennis P Pollow,Fenfen Liu,Michael Schlichting,Austin R Harrington,Bradley Liu,Thazha P Prakash,Elaine C Pirie,Han Zhu,Siyouneh Baghdasarian,Sean T Lee,Victor A Ruthig,Kristen L Wells,Johan Auwerx,Orian S Shirihai,Maike Sander

Developmental cell 58:522-534.e7 PubMed36924770

2023

Identifying constitutive and context-specific molecular-tension-sensitive protein recruitment within focal adhesions.

Applications

Unspecified application

Species

Unspecified reactive species

Arnold Tao,Andrew S LaCroix,T Curtis Shoyer,Vidya Venkatraman,Karen L Xu,Bradley Feiger,Brenton D Hoffman

Molecular medicine reports 22:2364-2372 PubMed32705202

2020

Asiaticoside produces an antidepressant‑like effect in a chronic unpredictable mild stress model of depression in mice, involving reversion of inflammation and the PKA/pCREB/BDNF signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Luoqing Wang,Ting Guo,Yuanfang Guo,Yujie Xu

The Journal of biological chemistry 295:9433-9444 PubMed32409581

2020

AKT Ser/Thr kinase increases V-ATPase-dependent lysosomal acidification in response to amino acid starvation in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Michael P Collins,Laura A Stransky,Michael Forgac

Nature genetics 47:647-53 PubMed25961942

2015

PDE3A mutations cause autosomal dominant hypertension with brachydactyly.

Applications

Unspecified application

Species

Unspecified reactive species

Philipp G Maass,Atakan Aydin,Friedrich C Luft,Carolin Schächterle,Anja Weise,Sigmar Stricker,Carsten Lindschau,Martin Vaegler,Fatimunnisa Qadri,Hakan R Toka,Herbert Schulz,Peter M Krawitz,Dmitri Parkhomchuk,Jochen Hecht,Irene Hollfinger,Yvette Wefeld-Neuenfeld,Eireen Bartels-Klein,Astrid Mühl,Martin Kann,Herbert Schuster,David Chitayat,Martin G Bialer,Thomas F Wienker,Jürg Ott,Katharina Rittscher,Thomas Liehr,Jens Jordan,Ghislaine Plessis,Jens Tank,Knut Mai,Ramin Naraghi,Russell Hodge,Maxwell Hopp,Lars O Hattenbach,Andreas Busjahn,Anita Rauch,Fabrice Vandeput,Maolian Gong,Franz Rüschendorf,Norbert Hübner,Hermann Haller,Stefan Mundlos,Nihat Bilginturan,Matthew A Movsesian,Enno Klussmann,Okan Toka,Sylvia Bähring
View all publications
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