AB194747

Anti-VASP (phospho S239) antibody

Name: Anti-VASP (phospho S239) antibody (ab194747) | Abcam
Brand: Abcam Limited
SKU: AB194747
Rating: 5 (1 reviews)

(1 Review)

(7 Publications)

Rabbit Polyclonal VASP phospho S239 antibody. Suitable for WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human Vasodilator-stimulated phosphoprotein phospho S239.

View Alternative Names

Vasodilator-stimulated phosphoprotein, VASP

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VASP (phospho S239) antibody (AB194747)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VASP (phospho S239) antibody (AB194747)

Immunohistochemistry of paraffin-embedded human placenta using ab194747 at dilution of 1 : 100.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VASP (phospho S239) antibody (AB194747)

Immunohistochemistry of paraffin-embedded rat spleen using ab194747 at dilution of 1 : 100.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-VASP (phospho S239) antibody (AB194747)

All lanes:

Western blot - Anti-VASP (phospho S239) antibody (ab194747) at 1/500 dilution

Lane 1:

Extract from serum-treated 293 cells

Lane 2:

Extract from PMA-treated C6 cells

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human, Mouse

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human Vasodilator-stimulated phosphoprotein phospho S239. The exact immunogen used to generate this antibody is proprietary information.

P50552

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VASP also known as Vasodilator-Stimulated Phosphoprotein is a 46-50 kDa protein involved in actin filament assembly and elongation. It plays an important role in cellular motility and the maintenance of cell shape. VASP is expressed in many cell types but is notably present in platelets and endothelial cells. It is an important player in signal transduction pathways that lead to cytoskeletal reorganization influencing cell movement and adhesion dynamics.

Biological function summary

VASP is an important component in cell movement regulation. It acts within a larger complex of actin-binding proteins that control the assembly of actin filaments. This protein influences cell adhesion migration and signal transduction processes. Through these actions VASP influences cellular responses to environmental cues making it essential for cellular dynamics and integrity during development and wound healing.

Pathways

VASP connects to significant signaling pathways such as the PI3K/Akt pathway and the cAMP pathway. It often interacts with proteins like c-Abl tyrosine kinase and Mena contributing to actin dynamics regulation. These pathways are critical for transducing signals from extracellular environments to intracellular responses allowing for coordinated cellular functions in processes such as cell migration and growth.

VASP plays a role in cardiovascular disease and cancer-related metastasis due to its function in cell motility and adhesion. Abnormal VASP activity is associated with impaired endothelial cell function and contributes to atherosclerosis development. Additionally it interacts with proteins like Mena and fascin in cancer cells aiding in metastatic progression by enhancing motility and invasive capabilities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.

See full target information Vasodilator-stimulated phosphoprotein phospho S239

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Molecular & cellular proteomics : MCP 22:100541 PubMed37019383

2023

Quantitative Proteomic Analysis Reveals apoE4-Dependent Phosphorylation of the Actin-Regulating Protein VASP.

Applications

Unspecified application

Species

Unspecified reactive species

Zeynep Cakir,Samuel J Lord,Yuan Zhou,Gwendolyn M Jang,Benjamin J Polacco,Manon Eckhardt,David Jimenez-Morales,Billy W Newton,Adam L Orr,Jeffrey R Johnson,Alexandre da Cruz,R Dyche Mullins,Nevan J Krogan,Robert W Mahley,Danielle L Swaney

Frontiers in oncology 11:716246 PubMed34745942

2021

High-Risk HPV16 E6 Activates the cGMP/PKG Pathway Through Glycosyltransferase ST6GAL1 in Cervical Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wang,Gao Liu,Mei Liu,Qinzhen Cai,Cong Yao,Hao Chen,Neng Song,Chunhui Yuan,Decai Tan,Yuhai Hu,Yun Xiang,Tian Xiang

Cell death & disease 12:150 PubMed33542225

2021

The novel ZEB1-upregulated protein PRTG induced by Helicobacter pylori infection promotes gastric carcinogenesis through the cGMP/PKG signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Xiang,Chunhui Yuan,Xia Guo,Honghao Wang,Qinzhen Cai,Yun Xiang,Wei Luo,Gao Liu

Microcirculation (New York, N.Y. : 1994) 27:e12608 PubMed31991513

2020

High-dose nitroglycerin administered during rewarming preserves erythrocyte deformability in cardiac surgery with cardiopulmonary bypass.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Hsuan Tai,You-Hsiang Chu,Hsiang-Ling Wu,Su-Man Lin,Mei-Yung Tsou,Cheng-Hsiung Huang,Hsiao-Huang Chang,Chih-Cherng Lu

Nitric oxide : biology and chemistry 78:1-10 PubMed29698689

2018

Nitric oxide induces epidermal stem cell de-adhesion by targeting integrin β1 and Talin via the cGMP signalling pathway.

Applications

Species

Unspecified reactive species

Rixing Zhan,Fan Wang,Ying Wu,Ying Wang,Wei Qian,Menglong Liu,Tengfei Liu,Weifeng He,Hui Ren,Gaoxing Luo

Proteomics 18:e1700242 PubMed29460479

2018

Label-Free Quantitative Phosphoproteomics Reveals Regulation of Vasodilator-Stimulated Phosphoprotein upon Stathmin-1 Silencing in a Pair of Isogenic Colorectal Cancer Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Hwee Tong Tan,Maxey Ching Ming Chung

Circulation research 122:405-416 PubMed29273600

2017

Deficiency of Natriuretic Peptide Receptor 2 Promotes Bicuspid Aortic Valves, Aortic Valve Disease, Left Ventricular Dysfunction, and Ascending Aortic Dilatations in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mark C Blaser,Kuiru Wei,Rachel L E Adams,Yu-Qing Zhou,Laura-Lee Caruso,Zahra Mirzaei,Alan Y-L Lam,Richard K K Tam,Hangjun Zhang,Scott P Heximer,R Mark Henkelman,Craig A Simmons

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VASP (phospho S239) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VASP (phospho S239) antibody (AB194747)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VASP (phospho S239) antibody (AB194747)

Western blot - Anti-VASP (phospho S239) antibody (AB194747)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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