Rabbit Polyclonal VASP phospho S239 antibody. Suitable for WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human Vasodilator-stimulated phosphoprotein phospho S239.
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- Molecular & cellular proteomics : MCP 22:1005412023Quantitative Proteomic Analysis Reveals apoE4-Dependent Phosphorylation of the Actin-Regulating Protein VASP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZeynep Cakir,Samuel J Lord,Yuan Zhou,Gwendolyn M Jang,Benjamin J Polacco,Manon Eckhardt,David Jimenez-Morales,Billy W Newton,Adam L Orr,Jeffrey R Johnson,Alexandre da Cruz,R Dyche Mullins,Nevan J Krogan,Robert W Mahley,Danielle L SwaneyPubMed 37019383
- Frontiers in oncology 11:7162462021High-Risk HPV16 E6 Activates the cGMP/PKG Pathway Through Glycosyltransferase ST6GAL1 in Cervical Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Wang,Gao Liu,Mei Liu,Qinzhen Cai,Cong Yao,Hao Chen,Neng Song,Chunhui Yuan,Decai Tan,Yuhai Hu,Yun Xiang,Tian XiangPubMed 34745942
- Cell death & disease 12:1502021The novel ZEB1-upregulated protein PRTG induced by Helicobacter pylori infection promotes gastric carcinogenesis through the cGMP/PKG signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTian Xiang et. al.PubMed 33542225
- Nitric oxide : biology and chemistry 78:1-102018Nitric oxide induces epidermal stem cell de-adhesion by targeting integrin β1 and Talin via the cGMP signalling pathway.
- Proteomics 18:e17002422018Label-Free Quantitative Phosphoproteomics Reveals Regulation of Vasodilator-Stimulated Phosphoprotein upon Stathmin-1 Silencing in a Pair of Isogenic Colorectal Cancer Cell Lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHwee Tong Tan,Maxey Ching Ming ChungPubMed 29460479
- Circulation research 122:405-4162017Deficiency of Natriuretic Peptide Receptor 2 Promotes Bicuspid Aortic Valves, Aortic Valve Disease, Left Ventricular Dysfunction, and Ascending Aortic Dilatations in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMark C Blaser,Kuiru Wei,Rachel L E Adams,Yu-Qing Zhou,Laura-Lee Caruso,Zahra Mirzaei,Alan Y-L Lam,Richard K K Tam,Hangjun Zhang,Scott P Heximer,R Mark Henkelman,Craig A SimmonsPubMed 29273600
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.91% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Vasodilator-stimulated phosphoprotein phospho S239. The exact immunogen used to generate this antibody is proprietary information. Database link P50552
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Expected |
| Rat | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
Alternative names
Vasodilator-stimulated phosphoprotein, VASP
Recommended products
Rabbit Polyclonal VASP phospho S239 antibody. Suitable for WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human Vasodilator-stimulated phosphoprotein phospho S239.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.91% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Vasodilator-stimulated phosphoprotein phospho S239. The exact immunogen used to generate this antibody is proprietary information. Database link P50552
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VASP also known as Vasodilator-Stimulated Phosphoprotein is a 46-50 kDa protein involved in actin filament assembly and elongation. It plays an important role in cellular motility and the maintenance of cell shape. VASP is expressed in many cell types but is notably present in platelets and endothelial cells. It is an important player in signal transduction pathways that lead to cytoskeletal reorganization influencing cell movement and adhesion dynamics.
- Biological function summary
VASP is an important component in cell movement regulation. It acts within a larger complex of actin-binding proteins that control the assembly of actin filaments. This protein influences cell adhesion migration and signal transduction processes. Through these actions VASP influences cellular responses to environmental cues making it essential for cellular dynamics and integrity during development and wound healing.
- Pathways
VASP connects to significant signaling pathways such as the PI3K/Akt pathway and the cAMP pathway. It often interacts with proteins like c-Abl tyrosine kinase and Mena contributing to actin dynamics regulation. These pathways are critical for transducing signals from extracellular environments to intracellular responses allowing for coordinated cellular functions in processes such as cell migration and growth.
- Associated diseases and disorders
VASP plays a role in cardiovascular disease and cancer-related metastasis due to its function in cell motility and adhesion. Abnormal VASP activity is associated with impaired endothelial cell function and contributes to atherosclerosis development. Additionally it interacts with proteins like Mena and fascin in cancer cells aiding in metastatic progression by enhancing motility and invasive capabilities.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-VASP (phospho S239) antibody (ab194747)
All lanes: Western blot - Anti-VASP (phospho S239) antibody (ab194747) at 1/500 dilution
Lane 1: Extract from serum-treated 293 cells
Lane 2: Extract from PMA-treated C6 cells
Predicted band size: 39 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VASP (phospho S239) antibody (ab194747)
Immunohistochemistry of paraffin-embedded rat spleen using ab194747 at dilution of 1:100.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VASP (phospho S239) antibody (ab194747)
Immunohistochemistry of paraffin-embedded human placenta using ab194747 at dilution of 1:100.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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