Rabbit Recombinant Monoclonal VASP phospho S239 antibody. Suitable for IP, Dot, WB and reacts with Human, Synthetic peptide samples.
Images
![Immunoprecipitation - Anti-VASP (phospho S239) antibody [EPR21734] (AB218619), expandable thumbnail](https://content.abcam.com/products/images/vasp-phospho-s239-antibody-epr21734-ab218619--immunoprecipitation-img4625.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-VASP (phospho S239) antibody [EPR21734] (AB218619)")
![Western blot - Anti-VASP (phospho S239) antibody [EPR21734] (AB218619), expandable thumbnail](https://content.abcam.com/products/images/vasp-phospho-s239-antibody-epr21734-ab218619--western-blot-img15035.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VASP (phospho S239) antibody [EPR21734] (AB218619)")
![Dot Blot - Anti-VASP (phospho S239) antibody [EPR21734] (AB218619), expandable thumbnail](https://content.abcam.com/products/images/vasp-phospho-s239-antibody-epr21734-ab218619--dot-blot-img13296.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-VASP (phospho S239) antibody [EPR21734] (AB218619)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Dot | WB | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Synthetic peptide | Not recommended | Tested | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
11 products for Alternative Product
1 products for Alternative Version
Target data
Function
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
Alternative names
Vasodilator-stimulated phosphoprotein, VASP, VASP
Recommended products
Rabbit Recombinant Monoclonal VASP phospho S239 antibody. Suitable for IP, Dot, WB and reacts with Human, Synthetic peptide samples.
Alternative names
Vasodilator-stimulated phosphoprotein, VASP, VASP
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR21734
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunoprecipitation - Anti-VASP (phospho S239) antibody [EPR21734] (ab218619), expandable thumbnail](https://content.abcam.com/products/images/vasp-phospho-s239-antibody-epr21734-ab218619--immunoprecipitation-img4625.jpg "Immunoprecipitation - Anti-VASP (phospho S239) antibody [EPR21734] (ab218619)")
Immunoprecipitation - Anti-VASP (phospho S239) antibody [EPR21734] (ab218619)
VASP (phospho S239) was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia cell line) treated with 10μM forskolin for 30min whole cell lysate using ab218619 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab218619 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1/1000 dilution.
Lane 1: THP-1 treated with 10μM forskolin for 30min whole cell lysate 10μg (input).
Lane 2: ab218619 IP in THP-1 treated with 10μM forskolin for 30min whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab218619 in THP-1 treated with 10μM forskolin for 30min whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.All lanes: Immunoprecipitation - Anti-VASP (phospho S239) antibody [EPR21734] (ab218619)
Predicted band size: 29 kDa, 33 kDa, 39 kDa, 56 kDa
Observed band size: 60 kDa
![Western blot - Anti-VASP (phospho S239) antibody [EPR21734] (ab218619), expandable thumbnail](https://content.abcam.com/products/images/vasp-phospho-s239-antibody-epr21734-ab218619--western-blot-img15035.jpg "Western blot - Anti-VASP (phospho S239) antibody [EPR21734] (ab218619)")
Western blot - Anti-VASP (phospho S239) antibody [EPR21734] (ab218619)
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a higher sensitivity ECL substrate.
All lanes: Western blot - Anti-VASP (phospho S239) antibody [EPR21734] (ab218619) at 1/1000 dilution
Lane 1: Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate at 10 µg
Lane 2: THP-1 treated with 10μM forskolin for 30 minutes, whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 39 kDa
Observed band size: 46 kDa
Exposure time: 3min
![Dot Blot - Anti-VASP (phospho S239) antibody [EPR21734] (ab218619), expandable thumbnail](https://content.abcam.com/products/images/vasp-phospho-s239-antibody-epr21734-ab218619--dot-blot-img13296.jpg "Dot Blot - Anti-VASP (phospho S239) antibody [EPR21734] (ab218619)")
Dot Blot - Anti-VASP (phospho S239) antibody [EPR21734] (ab218619)
Dot blot analysis of VASP (phospho S239) using ab218619 at 1/1000 dilution, followed byGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100,000 dilution.
Lane 1: VASP (phospho S239) peptide
Lane 2: VASP non-phospho peptide.Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 58 seconds.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com