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AB267470

Anti-Vaspin antibody [EPR23079-196]

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(1 Publication)

Rabbit Recombinant Monoclonal Vaspin antibody. Suitable for IP, WB and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

Serpin A12, Visceral adipose tissue-derived serine protease inhibitor, Visceral adipose-specific serpin, Vaspin, Serpina12

3 Images
Immunoprecipitation - Anti-Vaspin antibody [EPR23079-196] (AB267470)
  • IP

Unknown

Immunoprecipitation - Anti-Vaspin antibody [EPR23079-196] (AB267470)

Vaspin was immunoprecipitated from 0.35 mg Mouse stomach tissue lysate 10 with ab267470 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267470 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.

Lane 1 : Mouse stomach tissue lysate 10 ug

Lane 2 : ab267470 IP in Mouse stomach tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab267470 in mouse stomach tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 24 seconds

Vaspin protein can form polymers when heated as described in the literature (PMID : 28668641).

All lanes:

Immunoprecipitation - Anti-Vaspin antibody [EPR23079-196] (ab267470)

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

Western blot - Anti-Vaspin antibody [EPR23079-196] (AB267470)
  • WB

Lab

Western blot - Anti-Vaspin antibody [EPR23079-196] (AB267470)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 19554505).

Negative control : Mouse kidney (PMID : 19554505).

Exposure time : 26 seconds.

All lanes:

Western blot - Anti-Vaspin antibody [EPR23079-196] (ab267470) at 1/1000 dilution

Lane 1:

Mouse skin tissue lysate at 40 µg

Lane 2:

Mouse stomach tissue lysate at 40 µg

Lane 3:

Mouse kidney tissue lysate at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

Western blot - Anti-Vaspin antibody [EPR23079-196] (AB267470)
  • WB

Lab

Western blot - Anti-Vaspin antibody [EPR23079-196] (AB267470)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 26 seconds

All lanes:

Western blot - Anti-Vaspin antibody [EPR23079-196] (ab267470) at 1/1000 dilution

All lanes:

His-tagged recombinant mouse Vaspin protein (aa 20-413), 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

  • Carrier free

    Anti-Vaspin antibody [EPR23079-196] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23079-196

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

This antibody (Clone EPR23079-196) is a different clone to ab267471 (Clone EPR23080-232).

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues.
See full target information Serpin A12
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 25:1361-1386 PubMed38332150

2024

Sirt6 ablation in the liver causes fatty liver that increases cancer risky by upregulating Serpina12.

Applications

Unspecified application

Species

Unspecified reactive species

Licen Li,Jianming Zeng,Xin Zhang,Yangyang Feng,Josh Haipeng Lei,Xiaoling Xu,Qiang Chen,Chu-Xia Deng
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com