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AB252999

Anti-VAT1 antibody

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(2 Publications)

Rabbit Polyclonal VAT1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human VAT1 aa 100-200.

View Alternative Names

Synaptic vesicle membrane protein VAT-1 homolog, VAT1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAT1 antibody (AB252999)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAT1 antibody (AB252999)

Paraffin-embedded human urinary-bladder tissue stained for VAT1 using ab252999 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-VAT1 antibody (AB252999)
  • WB

Unknown

Western blot - Anti-VAT1 antibody (AB252999)

All lanes:

Western blot - Anti-VAT1 antibody (ab252999) at 0.4 µg/mL

All lanes:

RT4 (Human urinary bladder cancer cell line) cell lysate

Predicted band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human VAT1 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q99536

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VAT1 also known as Vesicle Amine Transport Protein 1 homolog is a protein involved mechanically in the transport and storage of amines in synaptic vesicles. It has an approximate mass of 45 kDa. VAT1 is highly expressed in the nervous system particularly in regions with a high density of synaptic vesicles. It localizes to the membrane of synaptic vesicles suggesting its role in vesicular trafficking and neurotransmitter transport processes.
Biological function summary

VAT1 functions in vesicular transport and neurotransmitter storage playing a role in synaptic transmission. This protein does not work alone; it contributes to a larger complex involved in establishing proper neurotransmitter concentrations within synaptic vesicles. VAT1 may also participate in modulating synaptic plasticity which influences learning and memory processes. Its activity ensures proper nerve signal transmission across synapses by maintaining neurotransmitter levels.

Pathways

The VAT1 protein is important in neurotransmitter release and recycling pathways in the nervous system. It associates with proteins like synaptophysin and synapsin I to facilitate vesicle docking and release. VAT1 plays a role in the synaptic vesicle cycle which includes vesicle loading exocytosis and recycling. Its function guarantees that neurotransmitters are quickly replenished promoting efficient synaptic communication.

VAT1 associates with neurological disorders such as Parkinson’s disease and epilepsy where neurotransmitter imbalances are evident. Connections between VAT1 and the protein alpha-synuclein are apparent in the context of Parkinson’s disease as disruptions in vesicular transport can lead to toxic protein aggregation. In epilepsy disturbances in VAT1 function can impact synaptic efficacy and neurotransmitter availability contributing to seizure activity. Understanding VAT1 function can provide insights into these disorders and offer targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Possesses ATPase activity (By similarity). Plays a part in calcium-regulated keratinocyte activation in epidermal repair mechanisms. Has no effect on cell proliferation. Negatively regulates mitochondrial fusion in cooperation with mitofusin proteins (MFN1-2).
See full target information VAT1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 10:836041 PubMed35281088

2022

iTRAQ-Based Proteome Profiling of Differentially Expressed Proteins in Insulin-Resistant Human Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yan,Bei Xie,Ye Tian,Li Huang,Shuli Zou,Zhiheng Peng,Zhuan Liu,Linjing Li

Neoplasma 68:416-422 PubMed33440991

2021

Expression of VAT1 in hepatocellular carcinoma and its clinical significance.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Heng,Fei Wei,Wanhai Li,Qian Li,Dan-Lei Xiong,Ying-Yu Ma,Da-Hong Zhang
View all publications
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