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AB40875

Anti-Vav proteins antibody [EP482Y]

5

(6 Reviews)

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(7 Publications)

Rabbit Recombinant Monoclonal VAV antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.

View Alternative Names

VAV, VAV1, Proto-oncogene vav

2 Images
Flow Cytometry (Intracellular) - Anti-Vav proteins antibody [EP482Y] (AB40875)
  • Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Vav proteins antibody [EP482Y] (AB40875)

Overlay histogram showing Jurkat cells stained with ab40875 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40875 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Western blot - Anti-Vav proteins antibody [EP482Y] (AB40875)
  • WB

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Western blot - Anti-Vav proteins antibody [EP482Y] (AB40875)

All lanes:

Western blot - Anti-Vav proteins antibody [EP482Y] (ab40875) at 1/2000 dilution

All lanes:

Jurkat cell lysate at 10 µg

Predicted band size: 98 kDa

Observed band size: 98 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP482Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p>Also 101 or 87 kDa, depending on the isoform.</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
See full target information VAV1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Microbiology spectrum 12:e0239123 PubMed38054722

2023

Vav proteins do not influence dengue virus replication but are associated with induction of phospho-ERK, IL-6, and viperin mRNA following DENV infection .

Applications

Unspecified application

Species

Unspecified reactive species

Evangeline Cowell,Hawraa Jaber,Luke P Kris,Madeleine G Fitzgerald,Valeria M Sanders,Aidan J Norbury,Nicholas S Eyre,Jillian M Carr

The Journal of allergy and clinical immunology 139:634-642.e5 PubMed27522155

2016

Mutations in linker for activation of T cells (LAT) lead to a novel form of severe combined immunodeficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Chiara Bacchelli,Federico A Moretti,Marlene Carmo,Stuart Adams,Horia C Stanescu,Kerra Pearce,Manisha Madkaikar,Kimberly C Gilmour,Adeline K Nicholas,C Geoffrey Woods,Robert Kleta,Phil L Beales,Waseem Qasim,H Bobby Gaspar

PLoS pathogens 9:e1003664 PubMed24130483

2013

Caspase-1 promotes Epstein-Barr virus replication by targeting the large tegument protein deneddylase to the nucleus of productively infected cells.

Applications

Unspecified application

Species

Human

Stefano Gastaldello,Xinsong Chen,Simone Callegari,Maria G Masucci

Science signaling 5:rs3 PubMed22534133

2012

Studying the dynamics of SLP-76, Nck, and Vav1 multimolecular complex formation in live human cells with triple-color FRET.

Applications

IP

Species

Unspecified reactive species

Maor H Pauker,Nirit Hassan,Elad Noy,Barak Reicher,Mira Barda-Saad

The Journal of cell biology 195:781-97 PubMed22123863

2011

Casein kinase I delta controls centrosome positioning during T cell activation.

Applications

ICC/IF

Species

Unspecified reactive species

Deborah Zyss,Hani Ebrahimi,Fanni Gergely

The EMBO journal 29:2315-28 PubMed20562827

2010

Cooperative interactions at the SLP-76 complex are critical for actin polymerization.

Applications

Unspecified application

Species

Unspecified reactive species

Mira Barda-Saad,Naoto Shirasu,Maor H Pauker,Nirit Hassan,Orly Perl,Andrea Balbo,Hiroshi Yamaguchi,Jon C D Houtman,Ettore Appella,Peter Schuck,Lawrence E Samelson

Molecular biology of the cell 19:2984-94 PubMed18463161

2008

The involvement of the tyrosine kinase c-Src in the regulation of reactive oxygen species generation mediated by NADPH oxidase-1.

Applications

WB

Species

Human

Davide Gianni,Ben Bohl,Sara A Courtneidge,Gary M Bokoch
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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