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Rabbit Recombinant Monoclonal VAV antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (AB247298), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (AB247298), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Also 101 or 87 kDa, depending on the isoform.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.

Alternative names

VAV, VAV1, Proto-oncogene vav

Recommended products

Rabbit Recombinant Monoclonal VAV antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EP482Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab247298 is the carrier-free version of Anti-Vav proteins antibody [EP482Y] ab40875.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (ab247298), expandable thumbnail

    Western blot - Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (ab247298)

    This data was developed using Anti-Vav proteins antibody [EP482Y] ab40875, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Vav proteins antibody [EP482Y] (Anti-Vav proteins antibody [EP482Y] ab40875) at 1/2000 dilution

    All lanes: Jurkat cell lysate at 10 µg

    Predicted band size: 98 kDa

    Observed band size: 98 kDa

  • Flow Cytometry (Intracellular) - Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (ab247298), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Vav proteins antibody [EP482Y] - BSA and Azide free (ab247298)

    This data was developed using Anti-Vav proteins antibody [EP482Y] ab40875, the same antibody clone in a different buffer formulation.Overlay histogram showing Jurkat cells stained with Anti-Vav proteins antibody [EP482Y] ab40875 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-Vav proteins antibody [EP482Y] ab40875, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com