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Rabbit Polyclonal VAV antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human VAV1 aa 50-550.

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Images

Western blot - Anti-VAV1 antibody (AB97574), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAV1 antibody (AB97574), expandable thumbnail
  • Western blot - Anti-VAV1 antibody (AB97574), expandable thumbnail
  • Western blot - Anti-VAV1 antibody (AB97574), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human VAV1 aa 50-550. The exact immunogen used to generate this antibody is proprietary information. Database link P15498

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Expected

Tested
Tested

Species

Mouse

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.

Alternative names

Recommended products

Rabbit Polyclonal VAV antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human VAV1 aa 50-550.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human VAV1 aa 50-550. The exact immunogen used to generate this antibody is proprietary information. Database link P15498
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

VAV1 also known as VAV-1 is a guanine nucleotide exchange factor with a mass of approximately 95 kDa. It activates Rho family GTPases by promoting the exchange of GDP for GTP. VAV1 is mainly expressed in hematopoietic cells playing an important role in immune responses. Its structure includes several domains – Dbl homology pleckstrin homology and an SH3 domain – that contribute to its function by facilitating interactions with other proteins.

Biological function summary

VAV1 regulates the signaling processes that lead to actin cytoskeletal rearrangements cell proliferation and differentiation. It forms part of larger multiprotein complexes that transmit signals from the cell surface to the nucleus. The activity of VAV1 is important for normal lymphocyte development and activation. By interacting with surface receptors it influences downstream effects altering cellular behavior and promoting adaptive immune responses.

Pathways

VAV1 is integral to the T cell receptor (TCR) signaling pathway and the B cell receptor (BCR) pathway. It acts as a link between the receptors and the Rho family of GTPases such as Rac1 and Cdc42 mediating the transmission of signals that result in cellular responses. The coupling of VAV1 with proteins like LAT and SLP-76 strengthens the signaling cascade necessary for lymphocyte activation and proliferation.

Associated diseases and disorders

Deregulation of VAV1 is associated with certain cancers such as leukemia and lymphoma. Changes in VAV1 expression or activity can disrupt normal immune cell signaling promoting unchecked growth and survival of malignant cells. Additionally VAV1 is linked to autoimmune diseases where its altered function can lead to inappropriate immune responses. VAV1 interactions with proteins like ZAP-70 highlight its role in these pathologies highlighting its significance as a potential therapeutic target.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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4 product images

  • Western blot - Anti-VAV1 antibody (ab97574), expandable thumbnail

    Western blot - Anti-VAV1 antibody (ab97574)

    7.5% SDS-PAGE

    All lanes: Western blot - Anti-VAV1 antibody (ab97574) at 1/500 dilution

    Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg

    Lane 2: Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

    Lane 3: NCI-H929 (human bone marrow lymphoblast cell line) whole cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 98 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAV1 antibody (ab97574), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAV1 antibody (ab97574)

    Paraffin-embedded human serous ovarian cancer tissue stained VAV1 using ab97574 at 1/100 dilution in immunohistochemical analysis.

    Antigen Retrieval: EDTA based buffer, pH 8.0, 15min

  • Western blot - Anti-VAV1 antibody (ab97574), expandable thumbnail

    Western blot - Anti-VAV1 antibody (ab97574)

    7.5% SDS-PAGE

    All lanes: Western blot - Anti-VAV1 antibody (ab97574) at 1/1000 dilution

    All lanes: BCL-1 (mouse B lymphocyte lymphoma cell line) whole cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 98 kDa

  • Western blot - Anti-VAV1 antibody (ab97574), expandable thumbnail

    Western blot - Anti-VAV1 antibody (ab97574)

    7.5% SDS-PAGE

    All lanes: Western blot - Anti-VAV1 antibody (ab97574) at 1/500 dilution

    Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg

    Lane 2: NCI-H1299 (human lung carcinoma cell line) whole cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 98 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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