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AB76225

Anti-VAV1 (phospho Y174) antibody [EP510Y]

5

(1 Review)

|

(19 Publications)

Rabbit Recombinant Monoclonal VAV phospho Y174 antibody. Suitable for Dot, WB, ICC/IF, Flow Cyt (Intra) and reacts with Synthetic peptide, Human samples. Cited in 19 publications.

View Alternative Names

VAV, VAV1, Proto-oncogene vav

4 Images
Flow Cytometry (Intracellular) - Anti-VAV1 (phospho Y174) antibody [EP510Y] (AB76225)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-VAV1 (phospho Y174) antibody [EP510Y] (AB76225)

Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) untreated/treated with 1mM pervanadate for 30min cells labeling VAV1 with unpurified ab76225 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. Untreated control (Green).

Immunocytochemistry/ Immunofluorescence - Anti-VAV1 (phospho Y174) antibody [EP510Y] (AB76225)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VAV1 (phospho Y174) antibody [EP510Y] (AB76225)

Immunocytochemistry/Immunofluorescence analysis of Untreated Jurkat cells and Pervanadate (1mM,30min) treated Jurkat cells labelling VAV1 (phospho Y174) with purified ab76225 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100, and counterstained with ab7291 anti-Tubulin (mouse mAb) 1 : 1000 (1ug/ml). An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (ab150077). Nuclei counterstained with DAPI (blue).

Negative Control 1 : Rabbit primary antibody and anti-mouse secondary antibody(ab150120)

Negative Control 2 : Mouse primary antibody(ab7291) and anti-rabbit secondary antibody(ab150077)

Western blot - Anti-VAV1 (phospho Y174) antibody [EP510Y] (AB76225)
  • WB

Lab

Western blot - Anti-VAV1 (phospho Y174) antibody [EP510Y] (AB76225)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-VAV1 (phospho Y174) antibody [EP510Y] (ab76225) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 10 µg

Lane 2:

Jurkat treated with 50mM pervanadate for 5minutes whole cell lysate at 10 µg

Lane 3:

Jurkat treated with 50mM pervanadate for 5min whole cell lysate 10 μg. Then the membrane was incubated with alkaline phosphatase at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 98 kDa

false

Dot Blot - Anti-VAV1 (phospho Y174) antibody [EP510Y] (AB76225)
  • Dot

Unknown

Dot Blot - Anti-VAV1 (phospho Y174) antibody [EP510Y] (AB76225)

Dot blot analysis of VAV1 (pY174) peptide (Lane 1) and VAV1 non-phospho peptide (Lane 2) labelling VAV1 (phospho Y174) with ab76225 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-VAV1 (phospho Y174) antibody [EP510Y]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-VAV1 (phospho Y174) antibody [EP510Y]

  • 519 FITC

    FITC Anti-VAV1 (phospho Y174) antibody [EP510Y]

  • Carrier free

    Anti-VAV1 (phospho Y174) antibody [EP510Y] - BSA and Azide free

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-VAV1 (phospho Y174) antibody [EP510Y]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-VAV1 (phospho Y174) antibody [EP510Y]

  • 578 PE

    PE Anti-VAV1 (phospho Y174) antibody [EP510Y]

  • 660 APC

    APC Anti-VAV1 (phospho Y174) antibody [EP510Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP510Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt (Intra), Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VAV1 also known as VAV-1 is a guanine nucleotide exchange factor with a mass of approximately 95 kDa. It activates Rho family GTPases by promoting the exchange of GDP for GTP. VAV1 is mainly expressed in hematopoietic cells playing an important role in immune responses. Its structure includes several domains – Dbl homology pleckstrin homology and an SH3 domain – that contribute to its function by facilitating interactions with other proteins.
Biological function summary

VAV1 regulates the signaling processes that lead to actin cytoskeletal rearrangements cell proliferation and differentiation. It forms part of larger multiprotein complexes that transmit signals from the cell surface to the nucleus. The activity of VAV1 is important for normal lymphocyte development and activation. By interacting with surface receptors it influences downstream effects altering cellular behavior and promoting adaptive immune responses.

Pathways

VAV1 is integral to the T cell receptor (TCR) signaling pathway and the B cell receptor (BCR) pathway. It acts as a link between the receptors and the Rho family of GTPases such as Rac1 and Cdc42 mediating the transmission of signals that result in cellular responses. The coupling of VAV1 with proteins like LAT and SLP-76 strengthens the signaling cascade necessary for lymphocyte activation and proliferation.

Deregulation of VAV1 is associated with certain cancers such as leukemia and lymphoma. Changes in VAV1 expression or activity can disrupt normal immune cell signaling promoting unchecked growth and survival of malignant cells. Additionally VAV1 is linked to autoimmune diseases where its altered function can lead to inappropriate immune responses. VAV1 interactions with proteins like ZAP-70 highlight its role in these pathologies highlighting its significance as a potential therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.
See full target information VAV1 phospho Y174
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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:332 PubMed38740744

2024

FOXP4-mediated induction of PTK7 activates the Wnt/β-catenin pathway and promotes ovarian cancer development.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Ji,Qilan Qian,Wenhao Cheng,Xiaoqing Ye,Aixin Jing,Shaojie Ma,Yuanyuan Ding,Xinhui Ma,Yasong Wang,Qian Sun,Xiujun Wang,Yulu Chen,Lan Zhu,Qing Yuan,Menghan Xu,Jingting Qin,Lin Ma,Jiayan Yang,Meiqi Zhang,Ting Geng,Sen Wang,Dan Wang,Yizhuo Song,Boyu Zhang,Yuting Xu,Linyu Xu,Shunfang Liu,Wei Liu,Bin Liu

International immunopharmacology 119:110177 PubMed37068336

2023

Esculin alleviates LPS-induced acute lung injury via inhibiting neutrophil recruitment and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangwei Ni,Ge Li,Ningfeng Dai,Zijiao Quan,Haibin Tong,Yu Liu

Cell reports 39:110695 PubMed35443168

2022

Oncogenic Vav1-Myo1f induces therapeutically targetable macrophage-rich tumor microenvironment in peripheral T cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jose R Cortes,Ioan Filip,Robert Albero,Juan A Patiño-Galindo,S Aidan Quinn,Wen-Hsuan W Lin,Anouchka P Laurent,Bobby B Shih,Jessie A Brown,Anisha J Cooke,Adam Mackey,Jonah Einson,Sakellarios Zairis,Alfredo Rivas-Delgado,Maria Antonella Laginestra,Stefano Pileri,Elias Campo,Govind Bhagat,Adolfo A Ferrando,Raul Rabadan,Teresa Palomero

Frontiers in medicine 8:675963 PubMed34414199

2021

Downregulation of CD45 Signaling in COVID-19 Patients Is Reversed by C24D, a Novel CD45 Targeting Peptide.

Applications

Unspecified application

Species

Unspecified reactive species

Danny Alon,Yossi Paitan,Eyal Robinson,Nirit Ganor,Julia Lipovetsky,Rinat Yerushalmi,Cyrille J Cohen,Annat Raiter

Oncoimmunology 10:1929725 PubMed34104545

2021

A novel role for an old target: CD45 for breast cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Annat Raiter,Oran Zlotnik,Julia Lipovetsky,Shany Mugami,Shira Dar,Ido Lubin,Eran Sharon,Cyrille J Cohen,Rinat Yerushalmi

The Journal of clinical investigation 130:3087-3097 PubMed32069268

2020

Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia Guedan,Aviv Madar,Victoria Casado-Medrano,Carolyn Shaw,Anna Wing,Fang Liu,Regina M Young,Carl H June,Avery D Posey

Cellular & molecular immunology 16:897-907 PubMed31316154

2019

Thymic-specific regulation of TCR signaling by Tespa1.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Lyu,Pengfei Wang,Ting Xu,Yichao Shen,Zejin Cui,Mingzhu Zheng,Guo Fu,Linrong Lu

Cancer letters 455:1-13 PubMed30981760

2019

CD100-plexin-B1 induces epithelial-mesenchymal transition of head and neck squamous cell carcinoma and promotes metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Zhang,Hongjiang Qiao,Weinan Guo,Yuan Liu,Luting Yang,Yufeng Liu,Boquan Jin,Meng Fu,Gang Wang,Wei Li

BMC immunology 20:7 PubMed30674283

2019

CEACAM1 regulates the IL-6 mediated fever response to LPS through the RP105 receptor in murine monocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Zhifang Zhang,Deirdre La Placa,Tung Nguyen,Maciej Kujawski,Keith Le,Lin Li,John E Shively

Cell stem cell 23:181-192.e5 PubMed30082067

2018

Human iPSC-Derived Natural Killer Cells Engineered with Chimeric Antigen Receptors Enhance Anti-tumor Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Li,David L Hermanson,Branden S Moriarity,Dan S Kaufman
View all publications
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