AB86695

Anti-VAV2 (phospho Y172) antibody

Name: Anti-VAV2 (phospho Y172) antibody (ab86695) | Abcam
Brand: Abcam Limited
SKU: AB86695

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(10 Publications)

Rabbit Polyclonal VAV2 phospho Y172 antibody. Suitable for WB and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human Guanine nucleotide exchange factor VAV2 phospho Y172 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Guanine nucleotide exchange factor VAV2, VAV-2, VAV2

1 Images

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Western blot - Anti-VAV2 (phospho Y172) antibody (AB86695)

All lanes:

Western blot - Anti-VAV2 (phospho Y172) antibody (ab86695) at 1/1000 dilution

Lane 1:

A431 cells treated with EGF

Lane 2:

A431 cells treated with EGF, exposed to alkaline phosphatase

Predicted band size: 101 kDa

Observed band size: 101 kDa,<100 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human Guanine nucleotide exchange factor VAV2 phospho Y172 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P52735

Specificity

ab86695 detects Vav1, Vav2, and Vav3 in Jurkat cells treated with pervanadate. The antibody does not detect these Vavs after treatment with alkaline phosphatase.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab86695 was cross-adsorbed to unphosphorylated VAV2 (Tyr-172) peptide before affinity purification using phospho-VAV2 (Tyr-172) peptide (without carrier).

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VAV2 also known as Vav 2 guanine nucleotide exchange factor is a member of the VAV family of proteins which function as guanine nucleotide exchange factors (GEFs). It has a molecular mass of approximately 100 kDa. The protein converts inactive GDP-bound Rho family GTPases such as Rac1 and Cdc42 into their active GTP-bound form. VAV2 is expressed in various tissues including the brain heart and lung indicating its broad role in cellular signaling across different systems.

Biological function summary

VAV2 influences multiple cellular processes through its role as a GEF. This protein does not form a part of a static complex but interacts dynamically with other signaling components. VAV2 regulates cytoskeleton organization cell migration and growth by activating Rho family GTPases. Its activity impacts processes such as neuronal development and immune response by modifying the actin cytoskeleton which is important for cell movement and morphological changes.

Pathways

VAV2 plays an integral role in signaling cascades like the Ras and MAPK pathways. It interacts closely with proteins like Rac1 and Cdc42 to propagate signals that influence cell proliferation and survival. By facilitating the switch from inactive GDP-bound states to active GTP-bound forms of Rho GTPases VAV2 integrates signals from cell surface receptors to downstream effectors that mediate cellular responses to external stimuli.

VAV2’s function connects to cancer progression and cardiovascular disorders. Changes in VAV2 expression or activity can lead to abnormal cell growth and differentiation linking it to cancerous transformations. Additionally because VAV2 modulates cell migration and cytoskeletal dynamics its malfunction can contribute to cardiovascular diseases by affecting vascular smooth muscle cells and endothelial cells. In both contexts VAV2's interactions with Rho family proteins like Rac1 highlights its influence in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity).

See full target information Guanine nucleotide exchange factor VAV2 phospho Y172

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Redox report : communications in free radical research 29:2324234 PubMed38444386

2024

Hyperglycemic stress induces oxidative damage of enteric glial cells by triggering redoxosomes/p66SHC activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yanmin Jiang,Lan Xu,Xue Zhu,Xiaowei Zhu,Xiang Xu,Jianbo Li

Cancers 14: PubMed36358781

2022

Adherens Junction Integrity Is a Critical Determinant of Sodium Iodide Symporter Residency at the Plasma Membrane of Thyroid Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Márcia Faria,José Vareda,Micaella Miranda,Maria João Bugalho,Ana Luísa Silva,Paulo Matos

Antioxidants (Basel, Switzerland) 11: PubMed35883759

2022

Extract Attenuates Light-Induced Photoreceptor Degeneration by Modulating CAV-1-Redoxosome Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Wang,Yuan Chen,Xue Zhu,Wenjun Zou,Fanfan Zhou

Experimental and therapeutic medicine 24:496 PubMed35837062

2022

Procyanidin B2 suppresses hyperglycemia-induced renal mesangial cell dysfunction by modulating CAV-1-dependent signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Yin,Ke Wang,Xue Zhu,Guoyuan Lu,Donghua Jin,Junsi Qiu,Fanfan Zhou

Cell death & disease 13:158 PubMed35177591

2022

Targeting CCL2-CCR4 axis suppress cell migration of head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zihang Ling,Wei Li,Jiaqi Hu,Yuanyuan Li,Miao Deng,Siyuan Zhang,Xianyue Ren,Tong Wu,Juan Xia,Bin Cheng,Xiaoan Tao

Theranostics 12:1303-1320 PubMed35154488

2022

Hedgehog signaling is controlled by Rac1 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Tang,Ximei Wu,Qianlei Ren,Minli Yao,Shouying Xu,Ziyi Yan

Experimental and therapeutic medicine 22:951 PubMed34335893

2021

extract protects human neuroblastoma SH-SY5Y cells against oxidative glutamate toxicity by activating redoxosome-p66Shc.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Wang,Jing Ni,Xue Zhu,Ling Zhu,Yue Li,Fanfan Zhou

Molecular oncology 9:1783-98 PubMed26105207

2015

Src family kinases differentially influence glioma growth and motility.

Applications

Unspecified application

Species

Unspecified reactive species

Laura J Lewis-Tuffin,Ryan Feathers,Priya Hari,Nisha Durand,Zhimin Li,Fausto J Rodriguez,Katie Bakken,Brett Carlson,Mark Schroeder,Jann N Sarkaria,Panos Z Anastasiadis

Oncogene 34:1979-90 PubMed24858039

2014

Vimentin regulates lung cancer cell adhesion through a VAV2-Rac1 pathway to control focal adhesion kinase activity.

Applications

Unspecified application

Species

Human

L S Havel,E R Kline,A M Salgueiro,A I Marcus

Journal of cell science 127:497-508 PubMed24284071

2013

PTP-PEST targets a novel tyrosine site in p120 catenin to control epithelial cell motility and Rho GTPase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Rosario Espejo,Yowjiun Jeng,Adriana Paulucci-Holthauzen,William Rengifo-Cam,Krysta Honkus,Panos Z Anastasiadis,Sarita K Sastry

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-VAV2 (phospho Y172) antibody

Western blot - Anti-VAV2 (phospho Y172) antibody (AB86695)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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