AB109544

Anti-VAV3 (phospho Y173) antibody [EPR1068A]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal VAV3 phospho Y173 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Guanine nucleotide exchange factor VAV3, VAV-3, VAV3

1 Images

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Western blot - Anti-VAV3 (phospho Y173) antibody [EPR1068A] (AB109544)

All lanes:

Western blot - Anti-VAV3 (phospho Y173) antibody [EPR1068A] (ab109544) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

Jurkat cell lysate treated with Pervanadate at 10 µg

Predicted band size: 98 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1068A

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The VAV3 protein also known as the guanine nucleotide exchange factor VAV3 functions as a regulator of Rho family GTPases by promoting the exchange of GDP for GTP. It weighs approximately 101 kDa and exhibits expression in various tissues including the brain heart and hematopoietic cells. VAV3 serves as an important modulator within the intracellular signaling processes by influencing actin cytoskeletal dynamics and impacting cellular morphology and movement.

Biological function summary

VAV3 influences multiple cellular processes like cell proliferation differentiation and migration. Part of many signaling complexes VAV3 interacts with receptors on the cell surface transmitting signals inside the cell. These interactions play a role in the regulation of immune responses and its involvement in the nervous system aids in neuron development and connectivity.

Pathways

VAV3 takes part in the regulation of the Rho GTPase pathway and the phosphoinositide 3-kinase (PI3K) pathway. VAV3 cooperates with proteins such as RAC1 and CDC42 influencing these pathways to modulate cell survival cytoskeleton rearrangement and membrane trafficking. Interaction with PI3K pathway components amplifies signals for growth factor-induced cellular responses impacting the overall cell function and homeostasis.

VAV3 influences both cancer and autoimmune diseases due to its regulatory role in cell signaling. Overexpression or abnormal regulation of VAV3 relates to prostate cancer progression and metastasis through its interaction with androgen receptor signaling pathways. In autoimmune diseases VAV3's interaction with proteins such as SYK can affect the severity of conditions like rheumatoid arthritis by modulating immune cell activation and response.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Exchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). May be important for integrin-mediated signaling, at least in some cell types. In osteoclasts, along with SYK tyrosine kinase, required for signaling through integrin alpha-v/beta-1 (ITAGV-ITGB1), a crucial event for osteoclast proper cytoskeleton organization and function. This signaling pathway involves RAC1, but not RHO, activation. Necessary for proper wound healing. In the course of wound healing, required for the phagocytotic cup formation preceding macrophage phagocytosis of apoptotic neutrophils. Responsible for integrin beta-2 (ITGB2)-mediated macrophage adhesion and, to a lesser extent, contributes to beta-3 (ITGB3)-mediated adhesion. Does not affect integrin beta-1 (ITGB1)-mediated adhesion (By similarity).

See full target information Guanine nucleotide exchange factor VAV3 phospho Y173

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:3056 PubMed35650206

2022

Nuclear Vav3 is required for polycomb repression complex-1 activity in B-cell lymphoblastic leukemogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

R C Nayak,K H Chang,A K Singh,M Kotliar,M Desai,A M Wellendorf,M Wunderlich,J Bartram,B Mizukawa,M Cuadrado,P Dexheimer,A Barski,X R Bustelo,N N Nassar,J A Cancelas

The Journal of biological chemistry 291:23854-23868 PubMed27681594

2016

Bone-protective Functions of Netrin 1 Protein.

Applications

Unspecified application

Species

Unspecified reactive species

Kenta Maruyama,Takahiko Kawasaki,Masahide Hamaguchi,Motomu Hashimoto,Moritoshi Furu,Hiromu Ito,Takao Fujii,Naoki Takemura,Thangaraj Karuppuchamy,Takeshi Kondo,Takumi Kawasaki,Masahiro Fukasaka,Takuma Misawa,Tatsuya Saitoh,Yutaka Suzuki,Mikaël M Martino,Yutaro Kumagai,Shizuo Akira

Molecular cancer research : MCR 11:1235-47 PubMed23938949

2013

Downregulation of HOPX controls metastatic behavior in sarcoma cells and identifies genes associated with metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Denisa Kovárová,Jirí Plachy,Jan Kosla,Katerina Trejbalová,Vladimír Čermák,Jiří Hejnar

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VAV3 (phospho Y173) antibody [EPR1068A]

Western blot - Anti-VAV3 (phospho Y173) antibody [EPR1068A] (AB109544)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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