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AB154855

Anti-VAX2 antibody [EPR10424]

1

(1 Review)

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(1 Publication)

Rabbit Recombinant Monoclonal VAX2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Ventral anterior homeobox 2, VAX2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-VAX2 antibody [EPR10424] (AB154855)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-VAX2 antibody [EPR10424] (AB154855)

Immunofluorescent analysis of Y79 cells labeling VAX2 with ab154855 at 1/100 dilution.

Western blot - Anti-VAX2 antibody [EPR10424] (AB154855)
  • WB

Unknown

Western blot - Anti-VAX2 antibody [EPR10424] (AB154855)

All lanes:

Western blot - Anti-VAX2 antibody [EPR10424] (ab154855) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Y79 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 31 kDa

false

  • Carrier free

    Anti-VAX2 antibody [EPR10424] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10424

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The alignment of the immunogen sequence of ab154855 and human and rat VAX1 shows a high homology of 92% (human) and 93% (rat). Based on the sequence, a cross-reaction of ab154855 with VAX1 is very likely, although this has not been experimentally tested by us.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VAX2 also known as Ventral Anterior Homeobox 2 is a transcription factor with a significant role in embryonic development. It has a molecular mass of around 36 kDa. VAX2 is primarily expressed in the developing central nervous system including the brain and eyes. This protein belongs to the homeobox gene family which regulates patterns of anatomical development.
Biological function summary

VAX2 controls the expression of genes involved in eye development. It is part of a transcriptional complex that includes proteins like VAX1 and PAX6. Together these proteins coordinate the formation of structures within the eye such as the retina. VAX2 ensures the correct spatial patterning and differentiation of photoreceptor cells maintaining proper eye morphogenesis.

Pathways

VAX2 plays a role in the retinal morphogenesis pathway and interacts closely with the Hedgehog signaling pathway. It works in tandem with proteins such as Sonic Hedgehog (SHH) to influence cellular differentiation and positional identity during development. VAX2's expression is regulated by these pathways demonstrating its integral function in developmental processes.

VAX2 has connections to eye disorders like coloboma and microphthalmia. These conditions are linked to mutations or dysregulations in genes that work alongside VAX2 such as PAX6. Any disruptions in VAX2 expression or function can lead to improper eye development potentially resulting in visual impairment or blindness. VAX2’s role highlights its significance in maintaining normal developmental processes and preventing congenital disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that may function in dorsoventral specification of the forebrain. Regulates the expression of Wnt signaling antagonists including the expression of a truncated TCF7L2 isoform that cannot bind CTNNB1 and acts therefore as a potent dominant-negative Wnt antagonist. Plays a crucial role in eye development and, in particular, in the specification of the ventral optic vesicle (By similarity). May be a regulator of axial polarization in the retina.
See full target information VAX2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 20:1314-1334 PubMed38174993

2024

Targeting cancer-associated fibroblast autophagy renders pancreatic cancer eradicable with immunochemotherapy by inhibiting adaptive immune resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaozhen Zhang,Mengyi Lao,Hanshen Yang,Kang Sun,Yunfei Dong,Lihong He,Xinchi Jiang,Honghui Wu,Yangwei Jiang,Muchun Li,Honggang Ying,Xinyuan Liu,Jian Xu,Yan Chen,Hanjia Zhang,Ruhong Zhou,Jianqing Gao,Xueli Bai,Tingbo Liang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com