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AB96085

Anti-VBP-1 antibody

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(2 Publications)

Rabbit Polyclonal VBP-1 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human VBP1 aa 1-150.

View Alternative Names

PFDN3, VBP1, Prefoldin subunit 3, HIBBJ46, von Hippel-Lindau-binding protein 1, VBP-1, VHL-binding protein 1

2 Images
Western blot - Anti-VBP-1 antibody (AB96085)
  • WB

Unknown

Western blot - Anti-VBP-1 antibody (AB96085)

12% SDS-PAGE

All lanes:

Western blot - Anti-VBP-1 antibody (ab96085) at 1/1000 dilution

All lanes:

A549 whole cell lysate at 30 µg

Predicted band size: 23 kDa

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Western blot - Anti-VBP-1 antibody (AB96085)
  • WB

Supplier Data

Western blot - Anti-VBP-1 antibody (AB96085)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-VBP-1 antibody (ab96085) at 1/1000 dilution

All lanes:

Mouse brain tissue lysate at 50 µg

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human VBP1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P61758

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VBP-1 also known as Von Hippel-Lindau binding protein 1 or prefoldin subunit 3 is a small protein with a molecular mass of approximately 23 kDa. It is expressed in various tissues including the brain heart and skeletal muscle. Mechanically VBP-1 functions as a molecular chaperone facilitating the proper folding of newly synthesized proteins and preventing the aggregation of misfolded proteins. It interacts with different molecular partners to exert its chaperoning activity.
Biological function summary

VBP-1 plays an important role in protein homeostasis particularly by forming part of the prefoldin complex. This complex works alongside the cytosolic chaperonin complex helping with the proper folding of cytoskeletal proteins such as actin and tubulin. By stabilizing these newly synthesized proteins VBP-1 supports cellular structures and intracellular transport systems reflecting its involvement in maintaining cellular integrity.

Pathways

VBP-1 plays a role in the ubiquitin-proteasome pathway and the cellular stress response pathway. In the ubiquitin-proteasome pathway it partners with proteins like HSP70 to target damaged proteins for degradation. In response to cellular stress VBP-1 helps in protein stabilization to prevent harmful aggregation correlating with HSP90 in maintaining cellular homeostasis during stress conditions.

VBP-1 has connections with neurodegenerative diseases such as Alzheimer's disease due to its role in protein folding and aggregation. Misregulation of VBP-1 can lead to the accumulation of neurotoxic protein aggregates. Additionally VBP-1 has been associated with cancer particularly through its interaction with pVHL (von Hippel-Lindau protein). Dysregulation in VBP-1 activity can result in the disruption of the ubiquitin-proteasome system leading to uncontrolled cell growth and cancer development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.
See full target information VBP1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The European journal of neuroscience 59:2102-2117 PubMed38279611

2024

Schizophrenia risk proteins ZNF804A and NT5C2 interact in cortical neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Afra Aabdien,Laura Sichlinger,Zoe Borgel,Madeleine R Jones,Iain A Waston,Nicholas J F Gatford,Pooja Raval,Lloyd Tanangonan,Timothy R Powell,Rodrigo R R Duarte,Deepak P Srivastava

Cancers 12: PubMed32344577

2020

Overexpression of Canonical Prefoldin Associates with the Risk of Mortality and Metastasis in Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xenia Peñate,Juan Manuel Praena-Fernández,Pedro Romero Pareja,María Del Valle Enguix-Riego,Laura Payán-Bravo,Begoña Vieites,Lourdes Gomez-Izquierdo,Javier Jaen Olasolo,Eleonor Rivin Del Campo,Jose Carlos Reyes,Sebastián Chávez,Jose Luis Lopez Guerra
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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