Anti-VCAM1 antibody [EPR27027-79] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VCAM1 antibody [EPR27027-79] - BSA and Azide free (AB316315)

This data was developed using ab316314, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Hut-78 (human Sezary syndrome cutaneous T lymphocyte) cells labelling VCAM1 with ab316314 at 1/50 (9.88 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing membranous and cytoplasmic staining in Hut-78 cell line. Nuclear DNA was labeled with DAPI.
Low expression : SK-OV-3 (PMID : 32934709).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-VCAM1 antibody [EPR27027-79] - BSA and Azide free (AB316315)

This data was developed using ab316314, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of SK-OV-3 (human ovarian cancer epithelial cell, Left) / Hut-78 (human Sezary syndrome cutaneous T lymphocyte, Right) cells labelling VCAM1 with ab316314 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Low expression : SK-OV-3. Gated on viable cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-VCAM1 antibody [EPR27027-79] - BSA and Azide free (AB316315)

This data was developed using ab316314, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of HUVEC (human umbilical vein endothelial cell) treated with 10ng/ml TNF-a for 16h (Red) / Untreated HUVEC (Dotted Red) cells labelling VCAM1 with ab316314 at 1/500 dilution (0.1 ug)/Red and Dotted Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-VCAM1 antibody [EPR27027-79] - BSA and Azide free (AB316315)

This data was developed using ab316314, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage, Left) / LADMAC (mouse bone marrow monocyte macrophage, Right) cells labelling VCAM1 with ab316314 at 1/5000 dilution (0.01 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Low expression : RAW 264.7. Gated on viable cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-VCAM1 antibody [EPR27027-79] - BSA and Azide free (AB316315)

This data was developed using ab316314, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse splenocytes cells labelling VCAM1 with ab316314 at 1/5000 dilution (0.01 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells are co-stained with CD3 conjugated to Alexa Fluor®647. The co-staining result showed the CD3 positive cluster has no cross activity with VCAM1.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-VCAM1 antibody [EPR27027-79] - BSA and Azide free (AB316315)

This data was developed using ab316314, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse splenocytes cells labelling VCAM1 with ab316314 at 1/5000 dilution (0.01 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells are co-stained with F4/80 conjugated to Alexa Fluor®647.

• Dot

Supplier Data

Dot Blot - Anti-VCAM1 antibody [EPR27027-79] - BSA and Azide free (AB316315)

This data was developed using ab316314, the same antibody clone in a different buffer formulation.

Dot blot analysis of VCAM1 using ab316314 at 1 : 1000 (0.494 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-VCAM1 antibody [EPR27027-79] (<a href='/en-us/products/primary-antibodies/vcam1-antibody-epr27027-79-ab316314'>ab316314</a>) at 1/1000 dilution

All lanes:

His-tagged human VCAM1 protein

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s