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Rabbit Recombinant Monoclonal VCAM1 antibody. Suitable for IP, WB and reacts with Mouse, Human samples. Cited in 26 publications.

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Images

Western blot - Anti-VCAM1 antibody [EPR5038(2)] (AB174279), expandable thumbnail
  • Western blot - Anti-VCAM1 antibody [EPR5038(2)] (AB174279), expandable thumbnail
  • Immunoprecipitation - Anti-VCAM1 antibody [EPR5038(2)] (AB174279), expandable thumbnail
  • Western blot - Anti-VCAM1 antibody [EPR5038(2)] (AB174279), expandable thumbnail
  • Western blot - Anti-VCAM1 antibody [EPR5038(2)] (AB174279), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Expected
Not recommended
Tested
Not recommended
Not recommended
Mouse
Tested
Not recommended
Tested
Not recommended
Not recommended
Rat
Predicted
Not recommended
Predicted
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/10 - 1/100
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/5000
Notes

Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for recommended treatment conditions and positive controls.

Species
Human
Dilution info
1/1000 - 1/5000
Notes

Stimulation may be required to allow detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for recommended treatment conditions and positive controls.

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed:31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed:10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed:22970700). Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed:35210567).

Alternative names

CD106, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1, INCAM-100, VCAM1

Recommended products

Rabbit Recombinant Monoclonal VCAM1 antibody. Suitable for IP, WB and reacts with Mouse, Human samples. Cited in 26 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR5038(2)
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.

Biological function summary

Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.

Pathways

VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.

Associated diseases and disorders

Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279), expandable thumbnail

    Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279) at 1/1000 dilution

    Lane 1: HUVEC (Human umbilical vein endothelial cell) whole cell lysate at 20 µg

    Lane 2: HUVEC treated with 10 ng/ml TNF-α for 16 h, whole cell lysate at 20 µg

    Lane 3: bEND.3 (Mouse brain endothelioma) whole cell lysate at 20 µg

    Lane 4: bEND.3 treated with 10 μg/ml LPS for 24 h, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 81 kDa

    Observed band size: 100 kDa

  • Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279), expandable thumbnail

    Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279) at 1/1000 dilution

    Lane 1: Hut-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate at 20 µg

    Lane 2: SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysate at 20 µg

    Lane 3: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 4: J774A.1 (Mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate at 20 µg

    Lane 5: LADMAC (Mouse bone marrow monocyte macrophage) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 81 kDa

    Observed band size: 100 kDa

    Exposure time: 15s

  • Immunoprecipitation - Anti-VCAM1 antibody [EPR5038(2)] (ab174279), expandable thumbnail

    Immunoprecipitation - Anti-VCAM1 antibody [EPR5038(2)] (ab174279)

    Immunoprecipitation. ab174279 at 1/1000 labeling VCAM1 immunoprecipitated from NIH3T3 cell lysate using ab174279 at 1/10.

    All lanes: Immunoprecipitation - Anti-VCAM1 antibody [EPR5038(2)] (ab174279)

    Predicted band size: 81 kDa

    Observed band size: 100 kDa

  • Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279), expandable thumbnail

    Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279)

    All lanes: Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279) at 1/1000 dilution

    Lane 1: Human fetal kidney lysate at 10 µg

    Lane 2: HuT-78 lysate at 10 µg

    Lane 3: NIH3T3 lysate at 10 µg

    Predicted band size: 81 kDa

  • Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279), expandable thumbnail

    Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279)

    ab174279 was shown to react with VCAM1 in treated wild-type A549 cells in western blot. Loss of signal was observed when treated VCAM1 knockout cell line Human VCAM1 knockout A549 cell line ab273758 (knockout cell lysate Human VCAM1 knockout A549 cell lysate ab275504) was used. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab174279 overnight at 4 °C at a 1 in 1000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1 in 5000 for 1 hour at room temperature before development with Optiblot ECL reagent (Anti-PKC delta (phospho S299) antibody [EPNCI119] ab133456) and imaging.

    All lanes: Western blot - Anti-VCAM1 antibody [EPR5038(2)] (ab174279) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 30 µg

    Lane 2: Wild-type A549 TNF-a treated (10 ng/mL, 16h) cell lysate at 30 µg

    Lane 3: VCAM1 knockout A549 cell lysate at 30 µg

    Lanes 3 - 4: Western blot - Human VCAM1 knockout A549 cell line (Human VCAM1 knockout A549 cell line ab273758)

    Lane 4: VCAM1 knockout A549 TNF-a treated (10 ng/mL, 16h) cell lysate at 30 µg

    Performed under reducing conditions.

    Predicted band size: 81 kDa

    Observed band size: 105 kDa

    Exposure time: 20s

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Product protocols

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