Rabbit Polyclonal VDAC1/Porin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 64 publications. Immunogen corresponding to Synthetic Peptide within Human VDAC1 aa 150-200.
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- International journal of molecular sciences 24:2023Regulatory Mechanism between Ferritin and Mitochondrial Reactive Oxygen Species in Spinal Ligament-Derived Cells from Ossification of Posterior Longitudinal Ligament Patient.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJong Tae Kim,Yonggoo Kim,Ji Yeon Kim,Seungok Lee,Myungshin Kim,Dong Wook JekarlPubMed 36769191
- International journal of molecular sciences 24:2023Cpt1c Downregulation Causes Plasma Membrane Remodelling and Anthracycline Resistance in Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHelena Muley,Karmele Valencia,Josefina Casas,Bea Moreno,Luis Botella,Fernando Lecanda,Rut Fadó,Núria CasalsPubMed 36674468
- Annals of clinical and translational neurology 9:1813-18192022Subsarcolemmal and cytoplasmic p62 positivity and rimmed vacuoles are distinctive for PLIN4-myopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Wang,Meng Yu,Wei Zhang,Qiang Gang,Zhiying Xie,Jin Xu,Chao Zhou,Depeng Wang,Lingchao Meng,He Lv,Zhirong Jia,Jianwen Deng,Yun Yuan,Zhaoxia WangPubMed 36151849
- Redox biology 55:1024002022Mitochondria-targeted anti-oxidant AntiOxCIN improved liver steatosis in Western diet-fed mice by preventing lipid accumulation due to upregulation of fatty acid oxidation, quality control mechanism and antioxidant defense systems.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRicardo Amorim,Inês C M Simões,José Teixeira,Fernando Cagide,Yaiza Potes,Pedro Soares,Adriana Carvalho,Ludgero C Tavares,Sofia Benfeito,Susana P Pereira,Rui F Simões,Agnieszka Karkucinska-Wieckowska,Ivan Viegas,Sylwia Szymanska,Michał Dąbrowski,Justyna Janikiewicz,Teresa Cunha-Oliveira,Agnieszka Dobrzyń,John G Jones,Fernanda Borges,Mariusz R Wieckowski,Paulo J OliveiraPubMed 35863265
- Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society 30:1387-13952022Celastrol and Rhynchophylline in the mitigation of simulated muscle atrophy under .Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaksymilian Wastag,Katarzyna Bieżuńska-Kusiak,Anna Szewczyk,Wojciech Szlasa,Bożena Grimling,Julita KulbackaPubMed 36387339
- The FEBS journal 289:5198-52172022Mitochondrial and metabolic remodelling in human skin fibroblasts in response to glucose availability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSónia A Pinho,Cláudio F Costa,Cláudia M Deus,Sonia L C Pinho,Inês Miranda-Santos,Gonçalo Afonso,Olivia Bagshaw,Jeffrey A Stuart,Paulo J Oliveira,Teresa Cunha-OliveiraPubMed 35213938
- International journal of molecular sciences 23:2022The Influence of Interaction between Cadmium with 17β-Estradiol, 2-Methoxyestradiol and 16α-Hydroxyestrone on Viability and p-Glycoprotein in Ovarian Cancer Cell Line.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEwa Sawicka,Jolanta Saczko,Julita Kulbacka,Martyna Szydełko,Beata Szymańska,Agnieszka PiwowarPubMed 35269769
- Redox biology 51:1022642022Voltage Dependent Anion Channel 3 (VDAC3) protects mitochondria from oxidative stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimona Reina,Stefano Conti Nibali,Marianna Flora Tomasello,Andrea Magrì,Angela Messina,Vito De PintoPubMed 35180474
- PeerJ 9:e125952021Inhibition of SARS-CoV-2 infection in human iPSC-derived cardiomyocytes by targeting the Sigma-1 receptor disrupts cytoarchitecture and beating.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJosé Alexandre Salerno,Thayana Torquato,Jairo R Temerozo,Livia Goto-Silva,Karina Karmirian,Mayara A Mendes,Carolina Q Sacramento,Natalia Fintelman-Rodrigues,Letícia R Q Souza,Isis M Ornelas,Carla P Veríssimo,Luiz Guilherme H S Aragão,Gabriela Vitória,Carolina S G Pedrosa,Suelen da Silva Gomes Dias,Vinicius Cardoso Soares,Teresa Puig-Pijuan,Vinícius Salazar,Rafael Dariolli,Diogo Biagi,Daniel R Furtado,Luciana Barreto Chiarini,Helena L Borges,Patrícia T Bozza,Marilia Zaluar P Guimarães,Thiago M L Souza,Stevens K RehenPubMed 35036128
- EMBO molecular medicine 13:e140722021Conformational change of adenine nucleotide translocase-1 mediates cisplatin resistance induced by EBV-LMP1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin Zhao,Xiangying Deng,Yueshuo Li,Jianmin Hu,Longlong Xie,Feng Shi,Min Tang,Ann M Bode,Xin Zhang,Weihua Liao,Ya CaoPubMed 34755470
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human VDAC1 aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link P21796
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Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rabbit, Cow, Pig, Zebrafish | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rabbit, Cow, Pig, Zebrafish | Dilution info - | Notes - |
Associated Products
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Target data
Function
Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane (PubMed:10661876, PubMed:11845315, PubMed:18755977, PubMed:30061676, PubMed:8420959). The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis (PubMed:10661876, PubMed:11845315, PubMed:18755977, PubMed:8420959). It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV (PubMed:10661876, PubMed:18755977, PubMed:8420959). The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed:18755977, PubMed:8420959). Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol (PubMed:18755977, PubMed:31015432). In depolarized mitochondria, acts downstream of PRKN and PINK1 to promote mitophagy or prevent apoptosis; polyubiquitination by PRKN promotes mitophagy, while monoubiquitination by PRKN decreases mitochondrial calcium influx which ultimately inhibits apoptosis (PubMed:32047033). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed:15033708, PubMed:25296756). May mediate ATP export from cells (PubMed:30061676). Part of a complex composed of HSPA9, ITPR1 and VDAC1 that regulates mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER lumen to the mitochondria intermembrane space thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix (By similarity). Mediates cytochrome c efflux (PubMed:20230784). Catalyzes the scrambling of phospholipids across the outer mitochondrial membrane; the mechanism is unrelated to channel activity and is capable of translocating both anionic and zwitterionic phospholipids.
Alternative names
VDAC, VDAC1, Non-selective voltage-gated ion channel VDAC1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, Voltage-dependent anion-selective channel protein 1, VDAC-1, hVDAC1
Recommended products
Rabbit Polyclonal VDAC1/Porin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 64 publications. Immunogen corresponding to Synthetic Peptide within Human VDAC1 aa 150-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human VDAC1 aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link P21796
- Purification technique
- Affinity purification Immunogen
- Specificity
Anti-VDAC/Porin Antibody is directed against human VDAC1/Porin1 protein. A BLAST analysis was used to suggest that this antibody would react with VDAC1/Porin1 from a wide range or organisms, including avian, mammalian, aquatic and reptilian sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms). Such broad reactivity makes this antibody useful as an excellent loading control (mitochondrial).
- Concentration
- Purification notes
This antibody was affinity purified from monospecific antiserum.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VDAC1 also known as Voltage-Dependent Anion Channel 1 or Porin is a channel protein with a mass around 31 kDa. It is located in the outer mitochondrial membrane and is express widely in various tissues. This protein forms a channel that allows the transport of metabolites and ions playing an important role in regulating energy and metabolic exchange between the mitochondria and the rest of the cell. Scientists often study it as a significant focus in cellular bioenergetics and apoptosis research.
- Biological function summary
VDAC1 acts as a pore-forming unit within the mitochondrial membrane allowing for the passage of small hydrophilic molecules. It is not part of a larger complex but works closely with other proteins to maintain mitochondrial function. VDAC1 regulates the entry and exit of proteins and ions essential for mitochondrial homeostasis and cellular energy production. By controlling the exchange of inorganic phosphate adenine nucleotides and Ca2+ VDAC1 influences both mitochondrial and cellular metabolism.
- Pathways
VDAC1 is involved in apoptosis and energy production pathways. It associates with proteins such as the Bcl-2 family in the apoptosis pathway influencing cell survival and programmed cell death. In energy production VDAC1 works in conjunction with the adenine nucleotide translocase facilitating ATP and ADP exchanges that are critical for maintaining cellular energy levels.
- Associated diseases and disorders
VDAC1 has a connection to cancer and neurodegenerative diseases. Upregulation or dysfunction of VDAC1 is observed in various cancers where it affects mitochondrial apoptosis regulation linked often with Bcl-2 anti-apoptotic proteins. In neurodegenerative diseases like Alzheimer’s alterations in VDAC1 expression and function can disrupt mitochondrial permeability. This interaction can result in disturbed neuronal energy metabolism often associated with amyloid precursor protein and its derivatives.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody - Mitochondrial Loading Control (ab34726)
VDAC1/Porin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-VDAC1/Porin antibody
IHC image of ab34726 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab34726, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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