Anti-VDAC1/Porin antibody [N152B/23] (ab186321) is a mouse monoclonal antibody that is used to detect VDAC1/Porin in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.
Images
![Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (AB186321), expandable thumbnail](https://content.abcam.com/products/images/vdac1-porin-antibody-n152b-23-mitochondrial-loading-control-ab186321--western-blot-img168960.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (AB186321)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (AB186321), expandable thumbnail](https://content.abcam.com/products/images/vdac1-porin-antibody-n152b-23-mitochondrial-loading-control-ab186321--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img185004.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (AB186321)")
![Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (AB186321), expandable thumbnail](https://content.abcam.com/products/images/vdac1-porin-antibody-n152b-23-mitochondrial-loading-control-ab186321--western-blot-img181388.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (AB186321)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (AB186321), expandable thumbnail](https://content.abcam.com/products/images/vdac1-porin-antibody-n152b-23-mitochondrial-loading-control-ab186321--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img169635.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (AB186321)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (AB186321), expandable thumbnail](https://content.abcam.com/products/images/vdac1-porin-antibody-n152b-23-mitochondrial-loading-control-ab186321--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img166988.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (AB186321)")
Publications
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- Life (Basel, Switzerland) 13:2023Molecular Investigation of Mitochondrial RNA19 Role in the Pathogenesis of MELAS Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaola Loguercio Polosa,Francesco Capriglia,Francesco BruniPubMed 37763267
- Cells 12:2023Impact of Translocator Protein 18 kDa (TSPO) Deficiency on Mitochondrial Function and the Inflammatory State of Human C20 Microglia Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefanie Bader,Thea Würfel,Tatjana Jahner,Caroline Nothdurfter,Rainer Rupprecht,Vladimir M Milenkovic,Christian H WetzelPubMed 36980295
- International journal of molecular sciences 23:2022Sirtuin 3 Deficiency Aggravates Kidney Disease in Response to High-Fat Diet through Lipotoxicity-Induced Mitochondrial Damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMonica Locatelli,Daniela Macconi,Daniela Corna,Domenico Cerullo,Daniela Rottoli,Giuseppe Remuzzi,Ariela Benigni,Carlamaria ZojaPubMed 35955472
- Cells 11:2022Shiga Toxin 2 Triggers C3a-Dependent Glomerular and Tubular Injury through Mitochondrial Dysfunction in Hemolytic Uremic Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimona Buelli,Monica Locatelli,Claudia Elisa Carminati,Daniela Corna,Domenico Cerullo,Barbara Imberti,Luca Perico,Maurizio Brigotti,Mauro Abbate,Carlamaria Zoja,Ariela Benigni,Giuseppe Remuzzi,Marina MorigiPubMed 35681450
- Cell death & disease 13:2962022Ultrastructural and proteomic profiling of mitochondria-associated endoplasmic reticulum membranes reveal aging signatures in striated muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue Lu,Yingchao Gong,Wanyu Hu,Yankai Mao,Ting Wang,Zeyu Sun,Xiaoling Su,Guosheng Fu,Yanpeng Wang,Dongwu LaiPubMed 35368021
- Journal of inflammation (London, England) 18:312021Ginsenoside Rg3 alleviates septic liver injury by regulating the lncRNA TUG1/miR-200c-3p/SIRT1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPan Wu,Xiao Yu,Yue Peng,Qian-Lu Wang,Long-Tian Deng,Wei XingPubMed 34930287
- Scientific reports 11:235802021Post-translational modifications by SIRT3 de-2-hydroxyisobutyrylase activity regulate glycolysis and enable nephrogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuca Perico,Marina Morigi,Anna Pezzotta,Daniela Corna,Valerio Brizi,Sara Conti,Cristina Zanchi,Fabio Sangalli,Piera Trionfini,Sara Buttò,Christodoulos Xinaris,Susanna Tomasoni,Carlamaria Zoja,Giuseppe Remuzzi,Ariela Benigni,Barbara ImbertiPubMed 34880332
- Nature communications 12:49322021The conformational stability of pro-apoptotic BAX is dictated by discrete residues of the protein core.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNoah B Bloch,Thomas E Wales,Michelle S Prew,Hannah R Levy,John R Engen,Loren D WalenskyPubMed 34389733
- JCI insight 5:2020C3a receptor blockade protects podocytes from injury in diabetic nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarina Morigi et. al.PubMed 32161193
- International journal of molecular sciences 20:2019CRISPR-Cas9 Mediated TSPO Gene Knockout alters Respiration and Cellular Metabolism in Human Primary Microglia Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVladimir M Milenkovic et. al.PubMed 31323920
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human VDAC1. Database link P21796
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Tested | Expected |
| Rat | Tested | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane (PubMed:10661876, PubMed:11845315, PubMed:18755977, PubMed:30061676, PubMed:8420959). The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis (PubMed:10661876, PubMed:11845315, PubMed:18755977, PubMed:8420959). It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV (PubMed:10661876, PubMed:18755977, PubMed:8420959). The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed:18755977, PubMed:8420959). Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol (PubMed:18755977, PubMed:31015432). In depolarized mitochondria, acts downstream of PRKN and PINK1 to promote mitophagy or prevent apoptosis; polyubiquitination by PRKN promotes mitophagy, while monoubiquitination by PRKN decreases mitochondrial calcium influx which ultimately inhibits apoptosis (PubMed:32047033). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed:15033708, PubMed:25296756). May mediate ATP export from cells (PubMed:30061676). Part of a complex composed of HSPA9, ITPR1 and VDAC1 that regulates mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER lumen to the mitochondria intermembrane space thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix (By similarity). Mediates cytochrome c efflux (PubMed:20230784). Catalyzes the scrambling of phospholipids across the outer mitochondrial membrane; the mechanism is unrelated to channel activity and is capable of translocating both anionic and zwitterionic phospholipids.
Alternative names
VDAC, VDAC1, Non-selective voltage-gated ion channel VDAC1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, Voltage-dependent anion-selective channel protein 1, VDAC-1, hVDAC1
Recommended products
Anti-VDAC1/Porin antibody [N152B/23] (ab186321) is a mouse monoclonal antibody that is used to detect VDAC1/Porin in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human VDAC1. Database link P21796
- Clone number
- N152B/23
- Purification technique
- Affinity purification Protein G
- Specificity
ab186321 shows weak cross reactivity with VDAC3.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-VDAC1/Porin antibody [N152B/23] (ab186321) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of VDAC1/Porin?
Anti-VDAC1/Porin [N152B/23] (ab186321) specifically detects a band for VDAC1/Porin (UniProt: P21796) at a molecular weight of 31kDa.
Trusted by the scientific community
Anti-VDAC1/Porin [N152B/23] (ab186321) was first used in a scientific publication in 2014 and has been cited over 10 times in peer-reviewed journals.
Reviewed by scientists
Anti-VDAC1/Porin [N152B/23] (ab186321) has over 5 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VDAC1 also known as Voltage-Dependent Anion Channel 1 or Porin is a channel protein with a mass around 31 kDa. It is located in the outer mitochondrial membrane and is express widely in various tissues. This protein forms a channel that allows the transport of metabolites and ions playing an important role in regulating energy and metabolic exchange between the mitochondria and the rest of the cell. Scientists often study it as a significant focus in cellular bioenergetics and apoptosis research.
- Biological function summary
VDAC1 acts as a pore-forming unit within the mitochondrial membrane allowing for the passage of small hydrophilic molecules. It is not part of a larger complex but works closely with other proteins to maintain mitochondrial function. VDAC1 regulates the entry and exit of proteins and ions essential for mitochondrial homeostasis and cellular energy production. By controlling the exchange of inorganic phosphate adenine nucleotides and Ca2+ VDAC1 influences both mitochondrial and cellular metabolism.
- Pathways
VDAC1 is involved in apoptosis and energy production pathways. It associates with proteins such as the Bcl-2 family in the apoptosis pathway influencing cell survival and programmed cell death. In energy production VDAC1 works in conjunction with the adenine nucleotide translocase facilitating ATP and ADP exchanges that are critical for maintaining cellular energy levels.
- Associated diseases and disorders
VDAC1 has a connection to cancer and neurodegenerative diseases. Upregulation or dysfunction of VDAC1 is observed in various cancers where it affects mitochondrial apoptosis regulation linked often with Bcl-2 anti-apoptotic proteins. In neurodegenerative diseases like Alzheimer’s alterations in VDAC1 expression and function can disrupt mitochondrial permeability. This interaction can result in disturbed neuronal energy metabolism often associated with amyloid precursor protein and its derivatives.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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8 product images
![Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321), expandable thumbnail](https://content.abcam.com/products/images/vdac1-porin-antibody-n152b-23-mitochondrial-loading-control-ab186321--western-blot-img168960.jpg "Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321)")
Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321)
VDAC1/Porin Western blot staining using mouse Anti-VDAC1/Porin antibody
Blocking peptide and diluting buffer: 5% NFDM /TBST
All lanes: Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321) at 1/1000 dilution
Lane 1: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2: HAP1 (Wildtype control Human chronic myelogenous leukemia near-haploid cell line) whole cell lysate at 20 µg
Lane 3: MEF (Mus musculus Embryo Fibroblast) whole cell lysate at 20 µg
Lane 4: Rat brain tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 33 kDa
Exposure time: 3s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321), expandable thumbnail](https://content.abcam.com/products/images/vdac1-porin-antibody-n152b-23-mitochondrial-loading-control-ab186321--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img185004.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321)
VDAC1/Porin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-VDAC1/Porin antibody
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling VDAC1 with ab186321 at 1/1000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection) at the supplied concentration. Dot staining on human tonsil is observed. Counter stained with hematoxylin. The section was incubated with ab186321 for 30 mins at room temperature, followed by an anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: PBS used instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).
![Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321), expandable thumbnail](https://content.abcam.com/products/images/vdac1-porin-antibody-n152b-23-mitochondrial-loading-control-ab186321--western-blot-img181388.jpg "Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321)")
Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321)
VDAC1/Porin Western blot staining using mouse Anti-VDAC1/Porin antibody
N-GST tagged Recombinant Human VDAC1 protein is available as Recombinant Human VDAC1/Porin protein ab132481
N-GST tagged Recombinant Human VDAC2 protein is available as Recombinant Human VDAC2 protein ab152793
Blocking peptide and diluting buffer: 5% NFDM /TBST
All lanes: Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321) at 1/1000 dilution
Lane 1: N-GST tagged Recombinant Human VDAC1 (aa1 to 283) protein at 0.01 µg
Lane 2: N-GST tagged Recombinant Human VDAC2 (aa 1 to 294) protein at 0.01 µg
Lane 3: N-GST tagged Recombinant Human VDAC3 (aa 1 to 283) protein at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 33 kDa
Exposure time: 80s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321), expandable thumbnail](https://content.abcam.com/products/images/vdac1-porin-antibody-n152b-23-mitochondrial-loading-control-ab186321--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img169635.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321)
VDAC1/Porin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-VDAC1/Porin antibody
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labelling VDAC1 with ab186321 at 1/1000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection) at the supplied concentration. Dot staining on human breast carcinoma is observed. Counter stained with hematoxylin. The section was incubated with ab186321 for 30 mins at room temperature, followed by an anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: PBS used instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321), expandable thumbnail](https://content.abcam.com/products/images/vdac1-porin-antibody-n152b-23-mitochondrial-loading-control-ab186321--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img166988.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321)
VDAC1/Porin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-VDAC1/Porin antibody
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labelling VDAC1 with ab186321 at 1/1000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection) at the supplied concentration. Dot staining on mouse kidney is observed. Counter stained with hematoxylin. The section was incubated with ab186321 for 30 mins at room temperature, followed by an anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: PBS used instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321), expandable thumbnail](https://content.abcam.com/products/images/vdac1-porin-antibody-n152b-23-mitochondrial-loading-control-ab186321--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img170017.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321)
VDAC1/Porin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-VDAC1/Porin antibody
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labelling VDAC1 with ab186321 at 1/1000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection) at the supplied concentration. Dot staining on rat kidney is observed. Counter stained with hematoxylin. The section was incubated with ab186321 for 30 mins at room temperature, followed by an anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: PBS used instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).
![Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321), expandable thumbnail](https://content.abcam.com/products/images/vdac1-porin-antibody-n152b-23-mitochondrial-loading-control-ab186321--immunocytochemistry-immunofluorescence-img401976.jpg "Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321)")
Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321)
VDAC1/Porin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-VDAC1/Porin antibody
Immunocytochemical analysis of 4% Paraformaldehyde-fixed human SH-SY5Y cells labelling VDAC1 with ab186321 at 1/100 dilution, followed by a secondary antibody (Alexa Fluor® 488) at 1/1000 dilution for 1 hour at room temperature.
Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VDAC1 Antibody (D) Composite.
![Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321), expandable thumbnail](https://content.abcam.com/products/images/vdac1-porin-antibody-n152b-23-mitochondrial-loading-control-ab186321--western-blot-img438305.jpg "Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321)")
Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321)
VDAC1/Porin Western blot staining using mouse Anti-VDAC1/Porin antibody
Blocking buffer: 5% Skim Milk powder in TBST.
All lanes: Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321) at 1/200 dilution
Lane 1: Mouse brain lysate
Lane 2: Human RT-4 lysate
SecondaryAll lanes: HRP Goat Anti-Mouse IgG at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321), expandable thumbnail](https://content.abcam.com/products/images/vdac1-porin-antibody-n152b-23-mitochondrial-loading-control-ab186321--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img170017.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321)")
![Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321), expandable thumbnail](https://content.abcam.com/products/images/vdac1-porin-antibody-n152b-23-mitochondrial-loading-control-ab186321--immunocytochemistry-immunofluorescence-img401976.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321)")
![Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321), expandable thumbnail](https://content.abcam.com/products/images/vdac1-porin-antibody-n152b-23-mitochondrial-loading-control-ab186321--western-blot-img438305.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321)")
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