Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control

• WB

Lab

Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (AB186321)

Blocking peptide and diluting buffer : 5% NFDM /TBST

All lanes:

Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321) at 1/1000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

HAP1 (Wildtype control Human chronic myelogenous leukemia near-haploid cell line) whole cell lysate at 20 µg

Lane 3:

MEF (Mus musculus Embryo Fibroblast) whole cell lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 31 kDa

Observed band size: 33 kDa

false

Exposure time: 3s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (AB186321)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labelling VDAC1 with ab186321 at 1/1000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection) at the supplied concentration. Dot staining on human breast carcinoma is observed. Counter stained with hematoxylin. The section was incubated with ab186321 for 30 mins at room temperature, followed by an anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control : PBS used instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (AB186321)

Immunocytochemical analysis of 4% Paraformaldehyde-fixed human SH-SY5Y cells labelling VDAC1 with ab186321 at 1/100 dilution, followed by a secondary antibody (Alexa Fluor® 488) at 1/1000 dilution for 1 hour at room temperature.

Counterstain : Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1 : 800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VDAC1 Antibody (D) Composite.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (AB186321)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling VDAC1 with ab186321 at 1/1000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection) at the supplied concentration. Dot staining on human tonsil is observed. Counter stained with hematoxylin. The section was incubated with ab186321 for 30 mins at room temperature, followed by an anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control : PBS used instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (AB186321)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labelling VDAC1 with ab186321 at 1/1000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection) at the supplied concentration. Dot staining on rat kidney is observed. Counter stained with hematoxylin. The section was incubated with ab186321 for 30 mins at room temperature, followed by an anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control : PBS used instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (AB186321)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labelling VDAC1 with ab186321 at 1/1000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection) at the supplied concentration. Dot staining on mouse kidney is observed. Counter stained with hematoxylin. The section was incubated with ab186321 for 30 mins at room temperature, followed by an anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control : PBS used instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

• WB

Lab

Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (AB186321)

N-GST tagged Recombinant Human VDAC1 protein is available as ab132481

N-GST tagged Recombinant Human VDAC2 protein is available as ab152793

Blocking peptide and diluting buffer : 5% NFDM /TBST

All lanes:

Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321) at 1/1000 dilution

Lane 1:

N-GST tagged Recombinant Human VDAC1 (aa1 to 283) protein at 0.01 µg

Lane 2:

N-GST tagged Recombinant Human VDAC2 (aa 1 to 294) protein at 0.01 µg

Lane 3:

N-GST tagged Recombinant Human VDAC3 (aa 1 to 283) protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 31 kDa

Observed band size: 33 kDa

false

Exposure time: 80s

• WB

Supplier Data

Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (AB186321)

Blocking buffer : 5% Skim Milk powder in TBST.

All lanes:

Western blot - Anti-VDAC1/Porin antibody [N152B/23] - Mitochondrial Loading Control (ab186321) at 1/200 dilution

Lane 1:

Mouse brain lysate

Lane 2:

Human RT-4 lysate

Secondary

All lanes:

HRP Goat Anti-Mouse IgG at 1/2000 dilution

Predicted band size: 30 kDa

Observed band size: 30 kDa

false