Skip to main content

Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) is a rabbit monoclonal antibody detecting VDAC1/Porin in Western Blot, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat.



- Biophysical QC for unrivalled batch-batch consistency

- Over 90 publications



Images

Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (AB154856), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (AB154856), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (AB154856), expandable thumbnail
  • Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (AB154856), expandable thumbnail
  • Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (AB154856), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFIHC-Fr
Human
Tested
Tested
Tested
Expected
Mouse
Tested
Tested
Expected
Tested
Rat
Tested
Expected
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane (PubMed:10661876, PubMed:11845315, PubMed:18755977, PubMed:30061676, PubMed:8420959). The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis (PubMed:10661876, PubMed:11845315, PubMed:18755977, PubMed:8420959). It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV (PubMed:10661876, PubMed:18755977, PubMed:8420959). The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed:18755977, PubMed:8420959). Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol (PubMed:18755977, PubMed:31015432). In depolarized mitochondria, acts downstream of PRKN and PINK1 to promote mitophagy or prevent apoptosis; polyubiquitination by PRKN promotes mitophagy, while monoubiquitination by PRKN decreases mitochondrial calcium influx which ultimately inhibits apoptosis (PubMed:32047033). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed:15033708, PubMed:25296756). May mediate ATP export from cells (PubMed:30061676). Part of a complex composed of HSPA9, ITPR1 and VDAC1 that regulates mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER lumen to the mitochondria intermembrane space thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix (By similarity). Mediates cytochrome c efflux (PubMed:20230784). Catalyzes the scrambling of phospholipids across the outer mitochondrial membrane; the mechanism is unrelated to channel activity and is capable of translocating both anionic and zwitterionic phospholipids.

Additional Targets

Voltage-dependent anion-selective channel protein 2

Alternative names

VDAC, VDAC1, Non-selective voltage-gated ion channel VDAC1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, Voltage-dependent anion-selective channel protein 1, VDAC-1, hVDAC1

Recommended products

Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) is a rabbit monoclonal antibody detecting VDAC1/Porin in Western Blot, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat.



- Biophysical QC for unrivalled batch-batch consistency

- Over 90 publications


Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR10852(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

What is this antibody validated in?


Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of VDAC1/Porin?


Anti-VDAC1/Porin + VDAC2 [EPR10852(B)] - Mitochondrial Loading Control (ab154856) is often used as a loading control in Western blot. It specifically detects a band for VDAC1/Porin (UniProt: P21796) at a molecular weight of 31kDa.

Trusted by the scientific community


Anti-VDAC1/Porin + VDAC2 [EPR10852(B)] - Mitochondrial Loading Control (ab154856) was first used in a scientific publication in 2012 and has been cited over 90 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed


The specificity of Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) has been confirmed by Western blot testing in VDAC1 Knockout HAP1 cell line, Human VDAC1 (Porin) knockout HEK-293T cell line ab255444.



Other related products


We have a range of other formats of antibody clone [EPR10852(B)] also available for your convenience:
ab154856, HRP - HRP Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control ab185063, Carrier free - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - BSA and Azide free ab240128, PE - PE Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control ab317896, APC - APC Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control ab317934, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control ab317974, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control ab318015, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control ab318057, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] – Mitochondrial Loading Control ab318099, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control ab321135



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The VDAC1/Porin and VDAC2 proteins also known as voltage-dependent anion-selective channel 1 and 2 serve as important pore-forming proteins within the mitochondrial outer membrane. Each VDAC protein has a molecular mass of about 30-35 kDa. Commonly referred to as the 'porin hub' due to their central role in metabolite exchange VDAC1 and VDAC2 facilitate the transport of ions and small molecules between the mitochondria and cytoplasm regulating energy production and cellular metabolism. They express widely across various tissues highlighting their essential role in cellular functions.

Biological function summary

Voltage-dependent anion channels like VDAC1/Porin and VDAC2 modulate the exchange of ions and metabolic substrates playing a significant role in apoptosis and the maintenance of mitochondrial functions. As integral parts of mitochondrial complexes they interact with proteins such as hexokinase which anchors to VDAC1 and influences glycolytic flux linking energy production with apoptotic signaling pathways. This positions VDAC proteins as key regulators of cellular energy homeostasis.

Pathways

VDAC1/Porin and VDAC2 engage prominently in the apoptotic and mitochondrial permeability transition pathways. In the apoptosis pathway they interact with anti-apoptotic proteins like Bcl-xl and pro-apoptotic members like Bax highlighting their dual role in cell survival and death. These interactions highlight the proteins’ contribution to mitochondrial outer membrane permeabilization (MOMP) a critical event in the release of cytochrome c and subsequent apoptosome formation further integrating them into broader signaling networks.

Associated diseases and disorders

Dysfunctions in VDAC1/Porin and VDAC2 link to neurodegenerative diseases and cancer. Aberrant VDAC1 activity connects with Alzheimer's disease due to its role in mitochondrial dysfunction affecting amyloid-beta peptide accumulation and neuronal cell death. In cancer VDAC overexpression associates with altered cellular metabolism and resistance to apoptosis positioning these proteins as potential therapeutic targets. Interactions with proteins such as hexokinase and Bcl-2 family members further emphasize their role in these pathological processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

20 product images

  • Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)

    All lanes: Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) at 1/5000 dilution

    All lanes: Rat cerebellum whole tissue lysate at 30 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97069)

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 31 kDa

    Exposure time: 2s

  • Immunohistochemistry (Frozen sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)

    Immunohistochemistry (Frozen sections) analysis of mouse skeletal muscle tissue sections labeling VDAC1 / Porin with Purified ab154856 at 1/50 (0.7 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)

    Immunohistochemical staining of paraffin embedded human cervical carcinoma with purified ab154856 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856), expandable thumbnail

    Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) at 1/1000 dilution

    Lanes 1 and 4: N-GST tagged full length recombinant human VDAC1 protein 10ng

    Lanes 2 and 5: N-GST tagged full length recombinant human VDAC2 protein 10ng

    Lanes 3 and 6: C-His tagged full length Recombinant Human VDAC3 protein 10ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 33 kDa, 55 kDa

    Exposure time: 40s

  • Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856), expandable thumbnail

    Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) at 1/10000 dilution

    Lane 1: HepG2 cell lysate at 20 µg

    Lane 2: HEK293 cell lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Observed band size: 31 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)

    ab154856 staining VDAC1 / Porin showing cytoplasmic staining in HeLa cells (Human cervix adenocarcinoma epithelial cells) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol, Samples were incubated with primary antibody (1/1000) for 1 hour at 21°C. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG (1:1000) was used as the secondary antibody. DAPI (1/200) was used as a counter stain.

  • Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856), expandable thumbnail

    Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) at 1/10000 dilution

    Lane 1: mouse kidney lysate at 20 µg

    Lane 2: rat kidney lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Observed band size: 31 kDa

  • Immunohistochemistry (Frozen sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)

    Immunohistochemistry (Frozen sections) analysis of mouse cardiac muscle tissue sections labeling VDAC1 / Porin with Purified ab154856 at 1/50 (0.7 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

  • Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856), expandable thumbnail

    Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)

    Secondary antibody - anti-rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab6721)

    All lanes: Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) at 1/1000 dilution

    Lane 1: HepG2 cell lysate at 10 µg

    Lane 2: Jurkat cell lysate at 10 µg

    Lane 3: 293T cell lysate at 10 µg

    Lane 4: HeLa cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

  • Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856), expandable thumbnail

    Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)

    ** Lanes 1 - 4:** Merged signal (red and green). Green - ab154856 observed at 31 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
    ab154856 was shown to react with VDAC1 / Porin in wild-type HEK-293T. Loss of signal was observed when knockout cell line Human VDAC1 (Porin) knockout HEK-293T cell line ab255444 (knockout cell lysate Human VDAC1 (Porin) knockout HEK-293T cell lysate ab263839) was used. Wild-type and VDAC1 / Porin knockout samples were subjected to SDS-PAGE. ab154856 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) at 1/1000 dilution

    Lane 1: Wild-type Hap1 cell lysate at 20 µg

    Lane 2: VDAC1 knockout Hap1 cell lysate at 20 µg

    Lane 3: Wild-type HEK-293T cell lysate at 20 µg

    Lane 4: VDAC1 knockout HEK-293T cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution

    Predicted band size: 31 kDa

    Observed band size: 124 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)

    ab154856 staining VDAC1 / Porin showing cytoplasmic staining in Jurkat cells (Human T cell leukemia T lymphocyte) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol, Samples were incubated with primary antibody (1/1000) for 1 hour at 21°C. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG (1:1000) was used as the secondary antibody. DAPI (1/200) was used as a counter stain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)

    Immunohistochemical staining of paraffin embedded rat kidney with purified ab154856 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)

    Immunohistochemical staining of paraffin embedded mouse cardiac muscle with purified ab154856 at a working dilution of 1/200. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856), expandable thumbnail

    Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) at 1/2000 dilution

    All lanes: Jurkat cell lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 22 kDa, 82 kDa, 98 kDa

    Observed band size: 24 kDa, 31 kDa, 82 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling VDAC1 with unpurified ab154856 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)

    Immunohistochemical analysis of paraffin-embedded human heart tissue labeling VDAC1 with unpurified ab154856 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)

    Immunohistochemical analysis of paraffin embedded human normal kidney tissue using unpurified ab154856 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)

    Immunohistochemical analysis of paraffin embedded human ovarian carcinoma tissue using unpurified ab154856 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)

    Immunohistochemical analysis of paraffin embedded human thyroid gland carcinoma tissue using unpurified ab154856 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856)

    Immunohistochemical analysis of paraffin embedded human skeletal muscle tissue using unpurified ab154856 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com