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Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734) is a mouse monoclonal antibody detecting VDAC1/Porin in Western Blot, Flow Cytometry, ICC/IF. Suitable for Cow, Human, Mouse, Rat.



- KO validated for confirmed specificity

- Over 580 publications

- Trusted since 2005


Images

Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (AB14734), expandable thumbnail
  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (AB14734), expandable thumbnail
  • Flow Cytometry - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (AB14734), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (AB14734), expandable thumbnail
  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (AB14734), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 0.36% HEPES

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected
Cat
Predicted
Predicted
Predicted
Catshark
Predicted
Predicted
Predicted
Common marmoset
Predicted
Predicted
Predicted
Cow
Expected
Tested
Expected
Dog
Predicted
Predicted
Predicted
Dogfish
Predicted
Predicted
Predicted
Drosophila melanogaster
Predicted
Predicted
Predicted
Fish
Predicted
Predicted
Predicted
Goat
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted
Quail
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1 µg for 106 Cells
Notes

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Sheep, Goat, Cat, Dog, Pig, Drosophila melanogaster, Fish, Quail, Common marmoset, Dogfish, Catshark
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1 µg/mL
Notes

-

Species
Rat
Dilution info
1 µg/mL
Notes

-

Species
Human
Dilution info
1 µg/mL
Notes

-

Species
Cow
Dilution info
1 µg/mL
Notes

-

Predicted
Predicted

Species
Sheep, Goat, Cat, Dog, Pig, Drosophila melanogaster, Fish, Quail, Common marmoset, Dogfish, Catshark
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat, Cow
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Sheep, Goat, Cat, Dog, Pig, Drosophila melanogaster, Fish, Quail, Common marmoset, Dogfish, Catshark
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane (PubMed:10661876, PubMed:11845315, PubMed:18755977, PubMed:30061676, PubMed:8420959). The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis (PubMed:10661876, PubMed:11845315, PubMed:18755977, PubMed:8420959). It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV (PubMed:10661876, PubMed:18755977, PubMed:8420959). The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed:18755977, PubMed:8420959). Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol (PubMed:18755977, PubMed:31015432). In depolarized mitochondria, acts downstream of PRKN and PINK1 to promote mitophagy or prevent apoptosis; polyubiquitination by PRKN promotes mitophagy, while monoubiquitination by PRKN decreases mitochondrial calcium influx which ultimately inhibits apoptosis (PubMed:32047033). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed:15033708, PubMed:25296756). May mediate ATP export from cells (PubMed:30061676). Part of a complex composed of HSPA9, ITPR1 and VDAC1 that regulates mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER lumen to the mitochondria intermembrane space thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix (By similarity). Mediates cytochrome c efflux (PubMed:20230784). Catalyzes the scrambling of phospholipids across the outer mitochondrial membrane; the mechanism is unrelated to channel activity and is capable of translocating both anionic and zwitterionic phospholipids.

Additional Targets

VDAC3

Alternative names

Recommended products

Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734) is a mouse monoclonal antibody detecting VDAC1/Porin in Western Blot, Flow Cytometry, ICC/IF. Suitable for Cow, Human, Mouse, Rat.



- KO validated for confirmed specificity

- Over 580 publications

- Trusted since 2005

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
20B12AF2
Purity
IgG fraction
Light chain type
kappa
Concentration
Loading...
Purification notes

Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

This antibody clone [20B12AF2] is manufactured by Abcam.

If you require this antibody in a different buffer formulation or a different conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Product Specifications
Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734) is a mouse monoclonal antibody and is validated for use in Flow Cyt, ICC/IF, WB in cow, human, mouse, rat samples.
Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734) specifically detects VDAC1/Porin + VDAC3 (UniProt ID: P21796; Molecular weight: 31kDa) and is sold in 100 µg selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734) has high sensitivity and specificity.
The specificity of Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734) has been confirmed by testing in knockout samples.
Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734) has been cited over 584 times in peer reviewed journals and is trusted by the scientific community.
Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] (ab14734) has 51 independent reviews from customers.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734), expandable thumbnail

    Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734)

    VDAC1/Porin + VDAC3 Western blot staining using mouse Anti-VDAC1/Porin + VDAC3 antibody

    Lanes 1-4: Merged signal (red and green). Green - ab14734 observed at 17 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 observed at 37 kDa.

    ab14734 was shown to react with VDAC1 / Porin in HEK-293T wildtype. Loss of signal was observed when knockout sample Human VDAC1 (Porin) knockout HEK-293T cell lysate ab263839 was used. Wild-type and VDAC1 / Porin knockout samples were subjected to SDS-PAGE. ab14734 and Anti-GAPDH antibody EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734) at 1/1000 dilution

    Lane 1: Wild-type Hap1 cell lysate at 20 µg

    Lane 2: VDAC1 knockout Hap1 cell lysate at 20 µg

    Lane 2: Western blot - Human VDAC1 (Porin) knockout HEK-293T cell line (Human VDAC1 (Porin) knockout HEK-293T cell line ab255444)

    Lane 3: Wild-type HEK-293T cell lysate at 20 µg

    Lane 4: VDAC1 knockout HEK-293T cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) at 1/20000 dilution

    Predicted band size: 31 kDa

    Observed band size: 17 kDa, 37 kDa

  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734), expandable thumbnail
    Li et al PLoS One. 2016 Feb 22;11(2):e0149728. doi: 10.1371/journal.pone.0149728. eCollection 2016. Fig 5.

    Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734)

    VDAC1/Porin + VDAC3 Western blot staining using mouse Anti-VDAC1/Porin + VDAC3 antibody

    Immunoblot analysis of endogenous VDAC1/Porin and VDAC3 in subcellular fractions of MA-10 cell lysates. VDAC1/Porin and VDAC3 are used as a mitochondrial marker proteins.

    Lane 1: Whole cell lysate

    Lane 2: Cytosolic fraction

    Lane 3: Mitochondrial-enriched fraction

    All lanes: Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734)

  • Flow Cytometry - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734), expandable thumbnail

    Flow Cytometry - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734)

    VDAC1/Porin + VDAC3 Flow Cytometry staining using mouse Anti-VDAC1/Porin + VDAC3 antibody

    Overlay histogram showing HepG2 (Human liver hepatocellular carcinoma cell line) cells stained with ab14734 (red line).

    The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14734, 1 μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (Mouse IgG2b [PLPV219] - Isotype Control ab91366, 2 μg/1x106 cells) used under the same conditions.

    Acquisition of >5,000 events was performed.

    This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734), expandable thumbnail
    This image is courtesy of a customer review by Michiel Krols.

    Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734)

    VDAC1/Porin + VDAC3 Immunocytochemistry/ Immunofluorescence staining using mouse Anti-VDAC1/Porin + VDAC3 antibody

    ab14734 staining VDAC1/Porin + VDAC3 in human HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 for 3 minutes and blocked with 0.2% serum for 60 minutes at 22°C. Samples were incubated with primary antibody (1/200 in 0.5% BSA and 0.02% Triton X100 in PBS) for 16 hours at 4°C. An FITC-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody at a dilution of 1/200.

  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734), expandable thumbnail

    Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734)

    VDAC1/Porin + VDAC3 Western blot staining using mouse Anti-VDAC1/Porin + VDAC3 antibody

    N-GST tagged Recombinant Human VDAC1 protein is available as Recombinant Human VDAC1/Porin protein ab132481

    N-GST tagged Recombinant Human VDAC2 protein is available as Recombinant Human VDAC2 protein ab152793

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734) at 1/1000 dilution

    Lane 1: N-GST tagged Recombinant Human VDAC1 (aa1 to 283) protein at 0.01 µg

    Lane 2: N-GST tagged Recombinant Human VDAC2 (aa 1 to 294) protein at 0.01 µg

    Lane 3: N-GST tagged Recombinant Human VDAC3 (aa 1 to 283) protein at 0.01 µg

    Observed band size: 33 kDa

    Exposure time: 10s

  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734), expandable thumbnail

    Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734)

    VDAC1/Porin + VDAC3 Western blot staining using mouse Anti-VDAC1/Porin + VDAC3 antibody

    Blocking and dilution buffer: 5% NFDM /TBST.

    All lanes: Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734) at 1/1000 dilution

    Lane 1: Wild type HAP1 whole cell lysate at 20 µg

    Lane 2: VDAC1 knockout HAP1 whole cell lysate at 20 µg

    Observed band size: 33 kDa

    Exposure time: 5s

  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734), expandable thumbnail

    Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734)

    VDAC1/Porin + VDAC3 Western blot staining using mouse Anti-VDAC1/Porin + VDAC3 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734) at 1/1000 dilution

    Lane 1: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 2: HAP1 (Wildtype control Human chronic myelogenous leukemia near-haploid cell line) whole cell lysate at 20 µg

    Lane 3: MEF (Mus musculus Embryo Fibroblast) whole cell lysate at 20 µg

    Lane 4: Rat brain tissue lysate at 20 µg

    Observed band size: 33 kDa

    Exposure time: 5s

  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734), expandable thumbnail
    Sun Y et al Voltage-dependent anion channels (VDACs) recruit Parkin to defective mitochondria to promote mitochondrial autophagy. J Biol Chem. 2012 Nov 23;287(48):40652-60. doi: 10.1074/jbc.M112.419721.

    Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734)

    VDAC1/Porin + VDAC3 Western blot staining using mouse Anti-VDAC1/Porin + VDAC3 antibody

    (A) The Anti-VDAC1/Porin + VDAC3 antibody recognizes VDAC1 and VDAC3 but not VDAC2.

    Lysates from wild-type MEFs and VDAC1/3-/- MEFs were analyzed by western blotting with Anti-VDAC1/Porin + VDAC3 (top panel) or anti Tom20 antibodies (bottom panel).

    (B) The Anti-VDAC1/Porin + VDAC3 antibody does not recognize VDAC2 in VDAC1/3-/- MEFs.

    HA-tagged VDAC1, 2 or 3 were expressed in VDAC1/3-/- MEFs, immunoprecipitated with anti-HA antibodies and analyzed by western blotting with anti-HA antibodies (top panel) or Anti-VDAC1/Porin + VDAC3 antibodies (bottom panel). The Anti-VDAC1/Porin + VDAC3 antibodies recognize HA-VDAC1 and HA-VDAC3, but not HA-VDAC2 . Note that HA-VDAC3 migrates at a lower molecular weight than HA-VDAC1 and HA-VDAC2 .

    Molecular weights are indicated in kDa.

    All lanes: Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734)

  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734), expandable thumbnail

    Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734)

    VDAC1/Porin + VDAC3 Western blot staining using mouse Anti-VDAC1/Porin + VDAC3 antibody

    Extra bands in the mouse sample (lane 4) are due to the reaction of the secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.

    All lanes: Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734)

    Lane 1: Isolated mitochondria from human heart at 15 µg

    Lane 2: Isolated mitochondria from bovine heart at 6 µg

    Lane 3: Isolated mitochondria from rat heart at 30 µg

    Lane 4: Isolated mitochondria from mouse heart at 30 µg

    Lane 5: HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 30 µg

    Observed band size: 37 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734)

    VDAC1/Porin + VDAC3 Immunocytochemistry/ Immunofluorescence staining using mouse Anti-VDAC1/Porin + VDAC3 antibody

    Immunofluoresence using ab14734 at 0.2 μg/ml on human fibroblasts (red).
    Nuclei were labeled with DAPI (blue).

  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734), expandable thumbnail
    Image courtesy of an anonymous customer review.

    Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734)

    VDAC1/Porin + VDAC3 Western blot staining of MCF7 (Human breast adenocarcinoma cell line) cell lysate using mouse Anti-VDAC1/Porin + VDAC3 antibody

    0.5% TBS-tween + Lait 5% NaN3 for 16 hours at 4°C.

    All lanes: Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734) at 1/500 dilution

    All lanes: MCF7 (Human breast adenocarcinoma cell line) cell lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-mouse polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 3min

  • Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734)

    VDAC1/Porin + VDAC3 Western blot staining using mouse Anti-VDAC1/Porin + VDAC3 antibody

    All lanes: Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (ab14734) at 1/5000 dilution

    Lanes 1 - 2: Rat brain cell lysate (homogenate) at 30 µg

    Lanes 3 - 4: Rat brain cell lysate (mitochondrial) at 30 µg

    Secondary

    All lanes: HRP conjugated sheep anti-mouse IgG

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 39 kDa

    Exposure time: 5min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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