Skip to main content

Rabbit Polyclonal VDAC2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human Voltage-dependent anion-selective channel protein 2 aa 200 to C-terminus.


Images

Western blot - Anti-VDAC2 antibody - C-terminal (AB155803), expandable thumbnail
  • Western blot - Anti-VDAC2 antibody - C-terminal (AB155803), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-VDAC2 antibody - C-terminal (AB155803), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human Voltage-dependent anion-selective channel protein 2 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P45880

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Mouse
Tested
Expected

Tested
Tested

Species

Mouse

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules (PubMed:38065946). The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV (By similarity). The open state has a weak anion selectivity whereas the closed state is cation-selective (By similarity). Binds various lipids, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol (PubMed:31015432). Binding of ceramide promotes the mitochondrial outer membrane permeabilization (MOMP) apoptotic pathway (PubMed:31015432).Catalyzes the scrambling of phospholipids across the outer mitochondrial membrane; the mechanism is unrelated to channel activity and is capable of translocating both anionic and zwitterionic phospholipids.

Alternative names

Recommended products

Rabbit Polyclonal VDAC2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human Voltage-dependent anion-selective channel protein 2 aa 200 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human Voltage-dependent anion-selective channel protein 2 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P45880
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Voltage-dependent anion channel 2 (VDAC2) also known as porin 31HL or P2 mechanically functions as a channel facilitating the transport of anions and metabolites across the outer mitochondrial membrane. This channel approximately 32 kDa in size is most prominent in the mitochondria but can also be observed in other cellular locations. It is involved in regulating both the entry and exit of small ions and molecules which is essential for mitochondrial function and energy production.

Biological function summary

VDAC2 plays significant roles beyond mere ion transport. It interacts with proteins involved in apoptosis such as Bcl-2 family proteins. Although not part of a larger complex its influence on the structure and permeability of the outer mitochondrial membrane impacts several cellular processes. The regulation of apoptosis by VDAC2 is key for cellular homeostasis and response to stress signals highlighting its role in maintaining normal cellular functions.

Pathways

Several cellular processes include VDAC2's role in apoptosis and energy metabolism. VDAC2 interacts with the intrinsic pathway of apoptosis in close association with proteins like Bax and Bak where it influences the release of cytochrome c from mitochondria. Additionally VDAC2 has a role in ATP transport and metabolic flux integral to the bioenergetics pathways by working with adenine nucleotide translocase (ANT) as part of the exchange of ATP and ADP.

Associated diseases and disorders

Mutations or dysregulation of VDAC2 connect to cancer and neurodegenerative diseases. Its interaction with the Bcl-2 family proteins implicates it in apoptosis resistance observed in various cancers. Likewise VDAC2 dysfunction has links to neurodegenerative diseases through its impact on mitochondrial health as these conditions often display an imbalance in apoptotic pathways and energy metabolism.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-VDAC2 antibody - C-terminal (ab155803), expandable thumbnail

    Western blot - Anti-VDAC2 antibody - C-terminal (ab155803)

    12% SDS PAGE

    All lanes: Western blot - Anti-VDAC2 antibody - C-terminal (ab155803) at 1/1000 dilution

    All lanes: Mouse liver whole cell lysate at 50 µg

    Predicted band size: 32 kDa

  • Western blot - Anti-VDAC2 antibody - C-terminal (ab155803), expandable thumbnail

    Western blot - Anti-VDAC2 antibody - C-terminal (ab155803)

    12% SDS PAGE

    All lanes: Western blot - Anti-VDAC2 antibody - C-terminal (ab155803) at 1/1000 dilution

    All lanes: HepG2 whole cell lysate at 30 µg

    Predicted band size: 32 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-VDAC2 antibody - C-terminal (ab155803), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-VDAC2 antibody - C-terminal (ab155803)

    Confocal immunofluorescence analysis of methanol-fixed HeLa cells labeling VDAC2 with ab155803 at 1/500 dilution (green). Alpha-tubulin filament labeling is shown in red.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com