JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB155803

Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control

5

(2 Reviews)

|

(3 Publications)

Rabbit Polyclonal VDAC2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human Voltage-dependent anion-selective channel protein 2 aa 200-300.

View Alternative Names

Non-selective voltage-gated ion channel VDAC2, VDAC-2, hVDAC2, Outer mitochondrial membrane protein porin 2, VDAC2

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (AB155803)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (AB155803)

Confocal immunofluorescence analysis of methanol-fixed HeLa cells labeling VDAC2 with ab155803 at 1/500 dilution (green). Alpha-tubulin filament labeling is shown in red.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (AB155803)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (AB155803)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue stained for VDAC2 protein with ab155803 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (AB155803)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (AB155803)

Immunohistochemical analysis of paraffin-embedded mouse intestine tissue stained for VDAC2 protein with ab155803 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (AB155803)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (AB155803)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue stained for VDAC2 protein with ab155803 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.

Western blot - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (AB155803)
  • WB

Supplier Data

Western blot - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (AB155803)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (ab155803) at 1/1000 dilution

Lane 1:

A549 whole cell extract at 30 µg

Lane 2:

H1299 whole cell extract at 30 µg

Lane 3:

HCT116 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 32 kDa

false

Western blot - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (AB155803)
  • WB

Supplier Data

Western blot - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (AB155803)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (ab155803) at 1/1000 dilution

Lane 1:

Mouse brain tissue extract at 50 µg

Lane 2:

Rat brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 32 kDa

false

Western blot - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (AB155803)
  • WB

Supplier Data

Western blot - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (AB155803)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (ab155803) at 1/500 dilution

All lanes:

Mouse liver tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Rat

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human Voltage-dependent anion-selective channel protein 2 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

P45880

style
left-column

Alternative Products

Primary Antibodies

AB126120

Anti-VDAC2 antibody - Mitochondrial Loading Control

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC2 antibody - Mitochondrial Loading Control (AB126120)

  • 0
  • 0
Primary Antibodies

AB118872

Anti-VDAC2 antibody - Mitochondrial Loading Control

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC2 antibody - Mitochondrial Loading Control (AB118872)

  • 0
  • 0
Primary Antibodies

AB37985

Anti-VDAC2 antibody - Mitochondrial Loading Control

Western blot - Anti-VDAC2 antibody - Mitochondrial Loading Control (AB37985)

  • 5
  • 8

Complementary Products

Reagents, Controls & Accessories

AB116029

Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)

  • 5
  • 1
Primary Antibodies

AB14734

Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control

Lab Essentials|KO Validated

Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (AB14734)

  • 5
  • 52
Primary Antibodies

AB16056

Anti-COX IV antibody - Mitochondrial Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody - Mitochondrial Loading Control (AB16056)

  • 5
  • 33
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Voltage-dependent anion channel 2 (VDAC2) also known as porin 31HL or P2 mechanically functions as a channel facilitating the transport of anions and metabolites across the outer mitochondrial membrane. This channel approximately 32 kDa in size is most prominent in the mitochondria but can also be observed in other cellular locations. It is involved in regulating both the entry and exit of small ions and molecules which is essential for mitochondrial function and energy production.
Biological function summary

VDAC2 plays significant roles beyond mere ion transport. It interacts with proteins involved in apoptosis such as Bcl-2 family proteins. Although not part of a larger complex its influence on the structure and permeability of the outer mitochondrial membrane impacts several cellular processes. The regulation of apoptosis by VDAC2 is key for cellular homeostasis and response to stress signals highlighting its role in maintaining normal cellular functions.

Pathways

Several cellular processes include VDAC2's role in apoptosis and energy metabolism. VDAC2 interacts with the intrinsic pathway of apoptosis in close association with proteins like Bax and Bak where it influences the release of cytochrome c from mitochondria. Additionally VDAC2 has a role in ATP transport and metabolic flux integral to the bioenergetics pathways by working with adenine nucleotide translocase (ANT) as part of the exchange of ATP and ADP.

Mutations or dysregulation of VDAC2 connect to cancer and neurodegenerative diseases. Its interaction with the Bcl-2 family proteins implicates it in apoptosis resistance observed in various cancers. Likewise VDAC2 dysfunction has links to neurodegenerative diseases through its impact on mitochondrial health as these conditions often display an imbalance in apoptotic pathways and energy metabolism.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane (PubMed : 8420959). The channel adopts an open conformation at zero mV and a closed conformation at both positive and negative potentials (PubMed : 8420959). There are two populations of channels; the main that functions in a lower open-state conductance with lower ion selectivity, that switch, in a voltage-dependent manner, from the open to a low-conducting 'closed' state and the other that has a normal ion selectivity in the typical high conductance, 'open' state (PubMed : 8420959). Binds various lipids, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol (PubMed : 31015432). Binding of ceramide promotes the mitochondrial outer membrane permeabilization (MOMP) apoptotic pathway (PubMed : 31015432). Associates with the translocase of the outer mitochondrial membrane (TOM) complex and PINK1 kinase at depolarized mitochondria, this interaction stabilizes PINK1 at the outer mitochondrial membrane and triggers downstream mitophagy by the recruitment of the E3 ubiquitin ligase PRKN (PubMed : 40080546).. Catalyzes the scrambling of phospholipids across the outer mitochondrial membrane; the mechanism is unrelated to channel activity and is capable of translocating both anionic and zwitterionic phospholipids.
See full target information Voltage-dependent anion-selective channel protein 2
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 27:438-448 PubMed39984653

2025

Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties.

Applications

Unspecified application

Species

Unspecified reactive species

Dennis K Jeppesen,Zachary C Sanchez,Noah M Kelley,James B Hayes,Jessica Ambroise,Emma N Koory,Evan Krystofiak,Nilay Taneja,Qin Zhang,Matthew M Dungan,Olivia L Perkins,Matthew J Tyska,Ela W Knapik,Kevin M Dean,Amanda C Doran,Robert J Coffey,Dylan T Burnette

Aging 16:3160-3184 PubMed38382091

2024

Single-cell and bulk RNA sequencing data jointly reveals s impacts on prognosis and immune landscape of NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Ma,Bateer Han,Qin Yu,Nashunbayaer Zha,Zhiyuan Deng,Junguo Liang,Rong Yu

Cell death & disease 15:40 PubMed38216558

2024

The E3 ubiquitin ligase Itch regulates death receptor and cholesterol trafficking to affect TRAIL-mediated apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

James Holloway,Aidan Seeley,Neville Cobbe,Richard C Turkington,Daniel B Longley,Emma Evergren
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com