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Rabbit Polyclonal VDAC2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human Voltage-dependent anion-selective channel protein 2 aa 200 to C-terminus.


Images

Western blot - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (AB155803), expandable thumbnail
  • Western blot - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (AB155803), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (AB155803), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Synthetic Peptide within Human Voltage-dependent anion-selective channel protein 2 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P45880

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Mouse
Tested
Expected

Tested
Tested

Species
Mouse
Dilution info
1/500.00000 - 1/3000.00000
Notes

-

Species
Human
Dilution info
1/500.00000 - 1/3000.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100.00000 - 1/1000.00000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules (PubMed:38065946). The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV (By similarity). The open state has a weak anion selectivity whereas the closed state is cation-selective (By similarity). Binds various lipids, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol (PubMed:31015432). Binding of ceramide promotes the mitochondrial outer membrane permeabilization (MOMP) apoptotic pathway (PubMed:31015432). Catalyzes the scrambling of phospholipids across the outer mitochondrial membrane; the mechanism is unrelated to channel activity and is capable of translocating both anionic and zwitterionic phospholipids.

Alternative names

Recommended products

Rabbit Polyclonal VDAC2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human Voltage-dependent anion-selective channel protein 2 aa 200 to C-terminus.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Synthetic Peptide within Human Voltage-dependent anion-selective channel protein 2 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P45880
Purification technique
Affinity purification Immunogen
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Voltage-dependent anion channel 2 (VDAC2) also known as porin 31HL or P2 mechanically functions as a channel facilitating the transport of anions and metabolites across the outer mitochondrial membrane. This channel approximately 32 kDa in size is most prominent in the mitochondria but can also be observed in other cellular locations. It is involved in regulating both the entry and exit of small ions and molecules which is essential for mitochondrial function and energy production.

Biological function summary

VDAC2 plays significant roles beyond mere ion transport. It interacts with proteins involved in apoptosis such as Bcl-2 family proteins. Although not part of a larger complex its influence on the structure and permeability of the outer mitochondrial membrane impacts several cellular processes. The regulation of apoptosis by VDAC2 is key for cellular homeostasis and response to stress signals highlighting its role in maintaining normal cellular functions.

Pathways

Several cellular processes include VDAC2's role in apoptosis and energy metabolism. VDAC2 interacts with the intrinsic pathway of apoptosis in close association with proteins like Bax and Bak where it influences the release of cytochrome c from mitochondria. Additionally VDAC2 has a role in ATP transport and metabolic flux integral to the bioenergetics pathways by working with adenine nucleotide translocase (ANT) as part of the exchange of ATP and ADP.

Associated diseases and disorders

Mutations or dysregulation of VDAC2 connect to cancer and neurodegenerative diseases. Its interaction with the Bcl-2 family proteins implicates it in apoptosis resistance observed in various cancers. Likewise VDAC2 dysfunction has links to neurodegenerative diseases through its impact on mitochondrial health as these conditions often display an imbalance in apoptotic pathways and energy metabolism.

Product promise

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3 product images

  • Western blot - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (ab155803), expandable thumbnail

    Western blot - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (ab155803)

    VDAC2 Western blot staining of Mouse liver whole cell lysate using rabbit Anti-VDAC2 antibody

    12% SDS PAGE

    All lanes: Western blot - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (ab155803) at 1/1000 dilution

    All lanes: Mouse liver whole cell lysate at 50 µg

    Predicted band size: 32 kDa

  • Western blot - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (ab155803), expandable thumbnail

    Western blot - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (ab155803)

    VDAC2 Western blot staining of HepG2 whole cell lysate using rabbit Anti-VDAC2 antibody

    12% SDS PAGE

    All lanes: Western blot - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (ab155803) at 1/1000 dilution

    All lanes: HepG2 whole cell lysate at 30 µg

    Predicted band size: 32 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (ab155803), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-VDAC2 antibody - C-terminal - Mitochondrial Loading Control (ab155803)

    VDAC2 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-VDAC2 antibody

    Confocal immunofluorescence analysis of methanol-fixed HeLa cells labeling VDAC2 with ab155803 at 1/500 dilution (green). Alpha-tubulin filament labeling is shown in red.

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Product protocols

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