Rabbit Polyclonal VDAC2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Voltage-dependent anion-selective channel protein 2 aa 1-200.
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Publications
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- Biochemical and biophysical research communication 505:1203-12102018Sulindac sulfone inhibits the mTORC1 pathway in colon cancer cells by directly targeting voltage-dependent anion channel 1 and 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuichi Aono et. al.PubMed 30327144
- Cell death & disease 9:9882018VDAC2 interacts with PFKP to regulate glucose metabolism and phenotypic reprogramming of glioma stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Zhou et. al.PubMed 30250190
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Voltage-dependent anion-selective channel protein 2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P45880
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat mediated antigen retrieval with 10mM Citrate buffer (PH6.0) or Tris-EDTA buffer ( PH8.0)). |
Associated Products
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Target data
Function
Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules (PubMed:38065946). The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV (By similarity). The open state has a weak anion selectivity whereas the closed state is cation-selective (By similarity). Binds various lipids, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol (PubMed:31015432). Binding of ceramide promotes the mitochondrial outer membrane permeabilization (MOMP) apoptotic pathway (PubMed:31015432). Catalyzes the scrambling of phospholipids across the outer mitochondrial membrane; the mechanism is unrelated to channel activity and is capable of translocating both anionic and zwitterionic phospholipids.
Alternative names
Voltage-dependent anion-selective channel protein 2, VDAC-2, hVDAC2, Outer mitochondrial membrane protein porin 2, VDAC2
Recommended products
Rabbit Polyclonal VDAC2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Voltage-dependent anion-selective channel protein 2 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Voltage-dependent anion-selective channel protein 2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P45880
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Voltage-dependent anion channel 2 (VDAC2) also known as porin 31HL or P2 mechanically functions as a channel facilitating the transport of anions and metabolites across the outer mitochondrial membrane. This channel approximately 32 kDa in size is most prominent in the mitochondria but can also be observed in other cellular locations. It is involved in regulating both the entry and exit of small ions and molecules which is essential for mitochondrial function and energy production.
- Biological function summary
VDAC2 plays significant roles beyond mere ion transport. It interacts with proteins involved in apoptosis such as Bcl-2 family proteins. Although not part of a larger complex its influence on the structure and permeability of the outer mitochondrial membrane impacts several cellular processes. The regulation of apoptosis by VDAC2 is key for cellular homeostasis and response to stress signals highlighting its role in maintaining normal cellular functions.
- Pathways
Several cellular processes include VDAC2's role in apoptosis and energy metabolism. VDAC2 interacts with the intrinsic pathway of apoptosis in close association with proteins like Bax and Bak where it influences the release of cytochrome c from mitochondria. Additionally VDAC2 has a role in ATP transport and metabolic flux integral to the bioenergetics pathways by working with adenine nucleotide translocase (ANT) as part of the exchange of ATP and ADP.
- Associated diseases and disorders
Mutations or dysregulation of VDAC2 connect to cancer and neurodegenerative diseases. Its interaction with the Bcl-2 family proteins implicates it in apoptosis resistance observed in various cancers. Likewise VDAC2 dysfunction has links to neurodegenerative diseases through its impact on mitochondrial health as these conditions often display an imbalance in apoptotic pathways and energy metabolism.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-VDAC2 antibody - Mitochondrial Loading Control (ab126120)
VDAC2 Western blot staining using rabbit Anti-VDAC2 antibody
12% SDS PAGE
All lanes: Western blot - Anti-VDAC2 antibody - Mitochondrial Loading Control (ab126120) at 1/1000 dilution
Lane 1: HepG2 whole cel lysate at 30 µg
Lane 2: HCT116 whole cell lysate at 30 µg
Predicted band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC2 antibody (ab126120)
ab126120, at 1/250 dilution, staining VDAC2 in paraffin embedded Human colon carcinoma tissue by Immunohistochemistry.
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