AB126120

Anti-VDAC2 antibody - Mitochondrial Loading Control

Name: Anti-VDAC2 antibody - Mitochondrial Loading Control (ab126120) | Abcam
Brand: Abcam Limited
SKU: AB126120
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal VDAC2 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Voltage-dependent anion-selective channel protein 2 aa 1-200.

View Alternative Names

Voltage-dependent anion-selective channel protein 2, VDAC-2, hVDAC2, Outer mitochondrial membrane protein porin 2, VDAC2

3 Images

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC2 antibody - Mitochondrial Loading Control (AB126120)

ab126120, at 1/250 dilution, staining VDAC2 in paraffin embedded Human colon carcinoma tissue by Immunohistochemistry.

Unknown

Western blot - Anti-VDAC2 antibody - Mitochondrial Loading Control (AB126120)

12% SDS PAGE

All lanes:

Western blot - Anti-VDAC2 antibody - Mitochondrial Loading Control (ab126120) at 1/1000 dilution

Lane 1:

HepG2 whole cel lysate at 30 µg

Lane 2:

HCT116 whole cell lysate at 30 µg

Predicted band size: 32 kDa

false

Supplier Data

Western blot - Anti-VDAC2 antibody - Mitochondrial Loading Control (AB126120)

Samples were separated by 12% SDS PAGE.

All lanes:

Western blot - Anti-VDAC2 antibody - Mitochondrial Loading Control (ab126120) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 50 µg

Lane 2:

Rat brain tissue lysates at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Voltage-dependent anion-selective channel protein 2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P45880

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Perform heat mediated antigen retrieval with 10mM Citrate buffer (PH6.0) or Tris-EDTA buffer ( PH8.0))." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Voltage-dependent anion channel 2 (VDAC2) also known as porin 31HL or P2 mechanically functions as a channel facilitating the transport of anions and metabolites across the outer mitochondrial membrane. This channel approximately 32 kDa in size is most prominent in the mitochondria but can also be observed in other cellular locations. It is involved in regulating both the entry and exit of small ions and molecules which is essential for mitochondrial function and energy production.

Biological function summary

VDAC2 plays significant roles beyond mere ion transport. It interacts with proteins involved in apoptosis such as Bcl-2 family proteins. Although not part of a larger complex its influence on the structure and permeability of the outer mitochondrial membrane impacts several cellular processes. The regulation of apoptosis by VDAC2 is key for cellular homeostasis and response to stress signals highlighting its role in maintaining normal cellular functions.

Pathways

Several cellular processes include VDAC2's role in apoptosis and energy metabolism. VDAC2 interacts with the intrinsic pathway of apoptosis in close association with proteins like Bax and Bak where it influences the release of cytochrome c from mitochondria. Additionally VDAC2 has a role in ATP transport and metabolic flux integral to the bioenergetics pathways by working with adenine nucleotide translocase (ANT) as part of the exchange of ATP and ADP.

Mutations or dysregulation of VDAC2 connect to cancer and neurodegenerative diseases. Its interaction with the Bcl-2 family proteins implicates it in apoptosis resistance observed in various cancers. Likewise VDAC2 dysfunction has links to neurodegenerative diseases through its impact on mitochondrial health as these conditions often display an imbalance in apoptotic pathways and energy metabolism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules (PubMed : 38065946). The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV (By similarity). The open state has a weak anion selectivity whereas the closed state is cation-selective (By similarity). Binds various lipids, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol (PubMed : 31015432). Binding of ceramide promotes the mitochondrial outer membrane permeabilization (MOMP) apoptotic pathway (PubMed : 31015432).. Catalyzes the scrambling of phospholipids across the outer mitochondrial membrane; the mechanism is unrelated to channel activity and is capable of translocating both anionic and zwitterionic phospholipids.

See full target information Voltage-dependent anion-selective channel protein 2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of health sciences 19:26-41 PubMed40046789

2025

Synergetic effect of doxorubicin and avenanthramide C on VDAC2/MTCH1 mitochondrial axis in breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ehab M M Ali,Sultan N Sonbul,Eman A Almuhaini,Ayat B Al-Ghafari

Biochemical and biophysical research communication 505:1203-1210 PubMed30327144

2018

Sulindac sulfone inhibits the mTORC1 pathway in colon cancer cells by directly targeting voltage-dependent anion channel 1 and 2.

Applications

Unspecified application

Species

Unspecified reactive species

Yuichi Aono,Mano Horinaka,Yosuke Iizumi,Motoki Watanabe,Tomoyuki Taniguchi,Shusuke Yasuda,Toshiyuki Sakai

Cell death & disease 9:988 PubMed30250190

2018

VDAC2 interacts with PFKP to regulate glucose metabolism and phenotypic reprogramming of glioma stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Zhou,Yue-Liang Yao,Zhi-Cheng He,Cong Chen,Xiao-Ning Zhang,Kai-Di Yang,Yu-Qi Liu,Qing Liu,Wen-Juan Fu,Ya-Ping Chen,Qin Niu,Qing-Hua Ma,Rong Zhou,Xiao-Hong Yao,Xia Zhang,You-Hong Cui,Xiu-Wu Bian,Yu Shi,Yi-Fang Ping

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-VDAC2 antibody - Mitochondrial Loading Control

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC2 antibody - Mitochondrial Loading Control (AB126120)

Western blot - Anti-VDAC2 antibody - Mitochondrial Loading Control (AB126120)

Western blot - Anti-VDAC2 antibody - Mitochondrial Loading Control (AB126120)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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