Anti-VE Cadherin antibody [11D4.1] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody [11D4.1] - BSA and Azide free (AB282291)

This data was developed using ab282277 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling VE Cadherin with ab282277 at 50 (17.04 ug/ml) dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on the vessels in mouse lung. The section was incubated with ab282277 overnight at 4 °C. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody [11D4.1] - BSA and Azide free (AB282291)

This data was developed using ab282277 the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling VE Cadherin with ab282277 at 50 (17.04 ug/ml) dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on the vessels in mouse spleen. The section was incubated with ab282277 overnight at 4 °C. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• WB

Lab

Western blot - Anti-VE Cadherin antibody [11D4.1] - BSA and Azide free (AB282291)

This data was developed using 282277, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Lysates were made freshly and used in WB immediately to minimize protein degradation.

The molecular weight observed is consistent with what has been described in the literature (PMID : 32732889)

Negative control : C2C12.

Exposure time : 1 second

All lanes:

Western blot - Anti-VE Cadherin antibody [11D4.1] (<a href='/en-us/products/primary-antibodies/ve-cadherin-antibody-11d41-ab282277'>ab282277</a>) at 1/1000 dilution

Lane 1:

bEnd.3 (mouse brain endothelioma), whole cell lysate at 20 µg

Lane 2:

C2C12 (mouse myoblasts myoblast), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Predicted band size: 88 kDa

Observed band size: 125 kDa,90 kDa

false