AB232880

Anti-VE Cadherin antibody

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(18 Publications)

Rabbit Polyclonal VE Cadherin antibody. Suitable for WB, IHC-P and reacts with Human, Cow samples. Cited in 18 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CDH5 aa 50-400.

View Alternative Names

CD144, Cadherin-5, 7B4 antigen, Vascular endothelial cadherin, VE-cadherin, CDH5

12 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody (AB232880)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody (AB232880)

Formalin-fixed, paraffin-embedded human liver tissue stained for VE Cadherin using ab232880 at 10 μg/mL in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal used at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody (AB232880)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody (AB232880)

Formalin-fixed, paraffin-embedded human lung tissue stained for VE Cadherin using ab232880 at 10 μg/mL in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal used at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody (AB232880)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody (AB232880)

Formalin-fixed, paraffin-embedded human lung tissue stained for VE Cadherin using ab232880 at 10 μg/mL in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal used at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody (AB232880)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody (AB232880)

Formalin-fixed, paraffin-embedded human prostate tissue stained for VE Cadherin using ab232880 at 10 μg/mL in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal used at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody (AB232880)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody (AB232880)

Formalin-fixed, paraffin-embedded human skin cancer tissue stained for VE Cadherin using ab232880 at 10 μg/mL in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal used at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody (AB232880)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody (AB232880)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for VE Cadherin using ab232880 at 10 μg/mL in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal used at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody (AB232880)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody (AB232880)

Formalin-fixed, paraffin-embedded human kidney tissue stained for VE Cadherin using ab232880 at 10 μg/mL in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal used at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody (AB232880)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody (AB232880)

Formalin-fixed, paraffin-embedded human skin cancer tissue stained for VE Cadherin using ab232880 at 10 μg/mL in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal used at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody (AB232880)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody (AB232880)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for VE Cadherin using ab232880 at 10 μg/mL in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal used at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody (AB232880)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody (AB232880)

Formalin-fixed, paraffin-embedded human kidney tissue stained for VE Cadherin using ab232880 at 10 μg/mL in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal used at 2 μg/ml. DAB staining.

Western blot - Anti-VE Cadherin antibody (AB232880)

WB

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Western blot - Anti-VE Cadherin antibody (AB232880)

All lanes:

Western blot - Anti-VE Cadherin antibody (ab232880) at 2 µg/mL

Lane 1:

Cow kidney tissue lysate

Lane 2:

Cow brain tissue lysate

Lane 3:

Cow spleen tissue lysate

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 88 kDa

false

Western blot - Anti-VE Cadherin antibody (AB232880)

WB

Supplier Data

Western blot - Anti-VE Cadherin antibody (AB232880)

All lanes:

Western blot - Anti-VE Cadherin antibody (ab232880) at 2 µg/mL

All lanes:

Recombinant human VE cadherin protein

Predicted band size: 88 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Cow, Human

Applications

IHC-P, WB

Immunogen

Recombinant Fragment Protein within Human CDH5 aa 50-400. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VE Cadherin also known as CD144 or Cadherin-5 is a protein of about 135 kDa involved in cell adhesion. It is a transmembrane protein characterized by its presence at endothelial cell junctions. VE Cadherin belongs to the cadherin superfamily and its expression predominantly occurs in vascular endothelial cells. This protein facilitates the adhesion between adjacent endothelial cells maintaining the integrity of endothelial barriers.

Biological function summary

VE Cadherin performs essential roles in vascular development and permeability. It is a component of adherens junctions and connects with catenins to mediate cell-cell adhesion through calcium-dependent binding. This protein's adhesive function is critical for blood vessel maturation and response to inflammatory stimuli. Altering VE Cadherin expression impacts vascular stability and endothelial cell layering.

Pathways

VE Cadherin interacts with the Wnt and VEGF signaling pathways. It plays a role in the regulation of endothelial cell survival and migration. The interaction with β-catenin is important in the Wnt pathway influencing the transcription of target genes that modulate cell behavior. In the VEGF pathway VE Cadherin's association with protein kinase C highlights its involvement in endothelial permeability and angiogenesis processes.

VE Cadherin dysregulation links to cancer progression and atherosclerosis. Its role in maintaining endothelial barrier integrity implicates it in tumor metastasis where disrupted cell adhesion facilitates cancer cell dissemination. Additionally altered VE Cadherin expression or function associates with atherosclerotic disease where damaged vascular endothelium leads to plaque formation. The connection of VE Cadherin with β-catenin in these conditions highlights its significance in pathological angiogenesis and vascular remodeling.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cadherins are calcium-dependent cell adhesion proteins (By similarity). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types (PubMed : 21269602). This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions (By similarity). It associates with alpha-catenin forming a link to the cytoskeleton (PubMed : 10861224). Plays a role in coupling actin fibers to cell junctions in endothelial cells, via acting as a cell junctional complex anchor for AMOTL2 and MAGI1 (By similarity). Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen (By similarity). These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B (PubMed : 20332120). Positively regulates reorientation of actin stress fibers and endothelial cell reorientation in response to cellular mechantransduction (PubMed : 25795300). Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction (PubMed : 20332120). Associates with CTNND1/p120-catenin to control CADH5 endocytosis (By similarity).

See full target information CDH5

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:574 PubMed40830966

2025

Bioengineered cardiovascular bypass grafts via in vivo self-assembly of scaffold-guided tubular tissue in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Shiqi Hu,Xiuhong Sun,Rui Zhou,Kai Fu,Yunfei Mo,Jialin Xu,Xiaoyan Shen,Shiwen Liu,Yuqing Niu

Iranian journal of biotechnology 22:e3693 PubMed39737205

2024

Inhibition of Chitinase-3-like Protein 1 Reduced Epithelial-Mesenchymal Transition and Vascular Epithelial Cadherin Expression in Oesophageal Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yanzi Qin,Wenjun Zhao,Qicai Li,Zhaogeng Cai,Guowen Wang,Nan Wang,Li Ma

ACS biomaterials science & engineering 10:7594-7605 PubMed39585331

2024

Engineering Granular Hydrogels without Interparticle Cross-Linking to Support Multicellular Organization.

Applications

Unspecified application

Species

Unspecified reactive species

Natasha L Claxton,Melissa A Luse,Brant E Isakson,Christopher B Highley

Function (Oxford, England) 5: PubMed38984993

2024

Endothelial-adipocyte Cx43 Mediated Gap Junctions Can Regulate Adiposity.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa A Luse,Luke S Dunaway,Shruthi Nyshadham,Alicia Carvalho,Meghan W Sedovy,Claire A Ruddiman,Rachel Tessema,Karen Hirschi,Scott R Johnstone,Brant E Isakson

Biofabrication 16: PubMed38941996

2024

A biopsy-sized 3D skin model with a perifollicular vascular plexus enables studying immune cell trafficking in the skin.

Applications

Unspecified application

Species

Unspecified reactive species

Krutav Rakesh Shah,Laura Garriga-Cerda,Alberto Pappalardo,Leila Sorrells,Hun Jin Jeong,Chang H Lee,Hasan Erbil Abaci

Stem cells international 2024:2172632 PubMed38681858

2024

Human Placental Mesenchymal Stem Cells-Exosomes Alleviate Endothelial Barrier Dysfunction via Cytoskeletal Remodeling through hsa-miR-148a-3p/ROCK1 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuzhen Lv,Wenqin Yu,Ruiui Xuan,Yulu Yang,Xiaolan Xue,Xiaowei Ma

Oxidative medicine and cellular longevity 2022:5772509 PubMed36105481

2022

Leukemia Inhibitory Factor Facilitates Self-Renewal and Differentiation and Attenuates Oxidative Stress of BMSCs by Activating PI3K/AKT Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Youde Liang,Ruiping Zhou,Xin Liu,Lin You,Chang Chen,Xiaoling Ye,Wei Wei,Jie Liu,Jiawei Dai,Kaixiong Li,Xiangxiang Zhao

Cancer gene therapy 29:1439-1451 PubMed35388172

2022

Exosomal HMGA2 protein from EBV-positive NPC cells destroys vascular endothelial barriers and induces endothelial-to-mesenchymal transition to promote metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Deng-Ke Li,Xing-Rui Chen,Li-Na Wang,Jia-Hong Wang,Ji-Ke Li,Zi-Ying Zhou,Xin Li,Lin-Bo Cai,Shui-Sheng Zhong,Jing-Jing Zhang,Yu-Mei Zeng,Qian-Bing Zhang,Xiao-Yan Fu,Xiao-Ming Lyu,Min-Ying Li,Zhong-Xi Huang,Kai-Tai Yao

Experimental and therapeutic medicine 23:313 PubMed35369532

2022

Glaucocalyxin A alleviates lipopolysaccharide-induced inflammation and apoptosis in pulmonary microvascular endothelial cells and permeability injury by inhibiting STAT3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jianwei Cao,Meiling Liu,Shufang Feng,Yingying Li,Kaijun Zheng

Experimental and therapeutic medicine 23:99 PubMed34976141

2022

lncRNA HOTAIR mediates OGD/R-induced cell injury and angiogenesis in a EZH2-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Yunpeng Wang,Jiaoyu Mao,Xiaodong Li,Beibei Wang,Xiaoguang Zhou

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