AB314094

Anti-VE-Cadherin antibody [BLR091G] - BSA free

Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal VE Cadherin antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CDH5 aa 1-600.

View Alternative Names

CD144, Cadherin-5, 7B4 antigen, Vascular endothelial cadherin, VE-cadherin, CDH5

5 Images

Supplier Data

Immunocytochemistry - Anti-VE-Cadherin antibody [BLR091G] - BSA free (AB314094)

Detection of human VE-Cadherin in FFPE HUVEC-C cells by ICC. Antibody : Rabbit anti-VE-Cadherin recombinant monoclonal antibody [BLR091G] (A700-091). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE-Cadherin antibody [BLR091G] - BSA free (AB314094)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE-Cadherin antibody [BLR091G] - BSA free (AB314094)

Detection of human VE-Cadherin in FFPE ovarian carcinoma by immunohistochemistry-IF. Antibody : Rabbit anti-VE-Cadherin recombinant monoclonal antibody [BLR091G] (A700-091). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : Opal™. Counterstain : DAPI.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE-Cadherin antibody [BLR091G] - BSA free (AB314094)

Detection of human VE-Cadherin in FFPE breast carcinoma by IHC. Antibody : Rabbit anti-VE-Cadherin recombinant monoclonal antibody [BLR091G] (A700-091 lot 1). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Supplier Data

Immunoprecipitation - Anti-VE-Cadherin antibody [BLR091G] - BSA free (AB314094)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from BeWo cells prepared using NETN lysis buffer. Antibodies : Rabbit anti-VE-Cadherin recombinant monoclonal antibody [BLR091G] (A700-091) used for IP at 6 µl per reaction. VE-Cadherin was also immunoprecipitated by rabbit anti-VE-Cadherin recombinant monoclonal antibody (BL1361-1E6). For blotting immunoprecipitated VE-Cadherin, A700-091 was used at 1 : 1000. Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-VE-Cadherin antibody [BLR091G] - BSA free (ab314094) at 1/1000 dilution

All lanes:

BeWo cells at 1 mg

false

Supplier Data

Western blot - Anti-VE-Cadherin antibody [BLR091G] - BSA free (AB314094)

Samples : Whole cell lysate from HeLa, HEK293T, BeWo, Jurkat, K-562, HUVEC-C, MCF-7, and Hep-G2 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-VE-Cadherin recombinant monoclonal antibody [BLR091G] (A700-091) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Detection : Chemiluminescence with an exposure time of 3 seconds. Lower Panel : Rabbit anti-COPB2.

All lanes:

Western blot - Anti-VE-Cadherin antibody [BLR091G] - BSA free (ab314094) at 1/1000 dilution

Lane 1:

HeLa Whole cell lysate at 50 µg

Lane 2:

HEK293T Whole cell lysate at 50 µg

Lane 3:

BeWo Whole cell lysate at 50 µg

Lane 4:

Jurkat Whole cell lysate at 50 µg

Lane 5:

K-562 Whole cell lysate at 50 µg

Lane 6:

HUVEC-C Whole cell lysate at 50 µg

Lane 7:

MCF-7 Whole cell lysate at 50 µg

Lane 8:

Hep-G2 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR091G

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, IP, ICC

applications

Immunogen

Recombinant Fragment Protein within Human CDH5 aa 1-600. The exact immunogen used to generate this antibody is proprietary information.

P33151

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VE Cadherin also known as CD144 or Cadherin-5 is a protein of about 135 kDa involved in cell adhesion. It is a transmembrane protein characterized by its presence at endothelial cell junctions. VE Cadherin belongs to the cadherin superfamily and its expression predominantly occurs in vascular endothelial cells. This protein facilitates the adhesion between adjacent endothelial cells maintaining the integrity of endothelial barriers.

Biological function summary

VE Cadherin performs essential roles in vascular development and permeability. It is a component of adherens junctions and connects with catenins to mediate cell-cell adhesion through calcium-dependent binding. This protein's adhesive function is critical for blood vessel maturation and response to inflammatory stimuli. Altering VE Cadherin expression impacts vascular stability and endothelial cell layering.

Pathways

VE Cadherin interacts with the Wnt and VEGF signaling pathways. It plays a role in the regulation of endothelial cell survival and migration. The interaction with β-catenin is important in the Wnt pathway influencing the transcription of target genes that modulate cell behavior. In the VEGF pathway VE Cadherin's association with protein kinase C highlights its involvement in endothelial permeability and angiogenesis processes.

VE Cadherin dysregulation links to cancer progression and atherosclerosis. Its role in maintaining endothelial barrier integrity implicates it in tumor metastasis where disrupted cell adhesion facilitates cancer cell dissemination. Additionally altered VE Cadherin expression or function associates with atherosclerotic disease where damaged vascular endothelium leads to plaque formation. The connection of VE Cadherin with β-catenin in these conditions highlights its significance in pathological angiogenesis and vascular remodeling.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Cadherins are calcium-dependent cell adhesion proteins (By similarity). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types (PubMed : 21269602). This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions (By similarity). It associates with alpha-catenin forming a link to the cytoskeleton (PubMed : 10861224). Plays a role in coupling actin fibers to cell junctions in endothelial cells, via acting as a cell junctional complex anchor for AMOTL2 and MAGI1 (By similarity). Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen (By similarity). These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B (PubMed : 20332120). Positively regulates reorientation of actin stress fibers and endothelial cell reorientation in response to cellular mechantransduction (PubMed : 25795300). Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction (PubMed : 20332120). Associates with CTNND1/p120-catenin to control CADH5 endocytosis (By similarity).

See full target information CDH5

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 15:677 PubMed39560824

2024

Flavokawain A suppresses the malignant progression of neuroblastoma in vitro depending on inactivation of ERK/VEGF/MMPs signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyao Dong,Gaiqin Li,Geng Geng,Ming Ming,Yongtao Xu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VE-Cadherin antibody [BLR091G] - BSA free

Immunocytochemistry - Anti-VE-Cadherin antibody [BLR091G] - BSA free (AB314094)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE-Cadherin antibody [BLR091G] - BSA free (AB314094)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE-Cadherin antibody [BLR091G] - BSA free (AB314094)

Immunoprecipitation - Anti-VE-Cadherin antibody [BLR091G] - BSA free (AB314094)

Western blot - Anti-VE-Cadherin antibody [BLR091G] - BSA free (AB314094)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise