Rabbit Recombinant Monoclonal VE Cadherin antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CDH5 aa 1-600.
Images
![Western blot - Anti-VE-Cadherin antibody [BLR091G] - BSA free (AB314094), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-blr091g-bsa-free-ab314094--western-blot-img420616.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VE-Cadherin antibody [BLR091G] - BSA free (AB314094)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE-Cadherin antibody [BLR091G] - BSA free (AB314094), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-blr091g-bsa-free-ab314094--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420619.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE-Cadherin antibody [BLR091G] - BSA free (AB314094)")
![Immunocytochemistry - Anti-VE-Cadherin antibody [BLR091G] - BSA free (AB314094), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-blr091g-bsa-free-ab314094--immunocytochemistry-img420620.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-VE-Cadherin antibody [BLR091G] - BSA free (AB314094)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE-Cadherin antibody [BLR091G] - BSA free (AB314094), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-blr091g-bsa-free-ab314094--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420618.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE-Cadherin antibody [BLR091G] - BSA free (AB314094)")
![Immunoprecipitation - Anti-VE-Cadherin antibody [BLR091G] - BSA free (AB314094), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-blr091g-bsa-free-ab314094--immunoprecipitation-img420617.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-VE-Cadherin antibody [BLR091G] - BSA free (AB314094)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human CDH5 aa 1-600. The exact immunogen used to generate this antibody is proprietary information. Database link P33151
Reactivity data
Select an application| ICC | IHC-P | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Cadherins are calcium-dependent cell adhesion proteins (By similarity). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types (PubMed:21269602). This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions (By similarity). It associates with alpha-catenin forming a link to the cytoskeleton (PubMed:10861224). Plays a role in coupling actin fibers to cell junctions in endothelial cells, via acting as a cell junctional complex anchor for AMOTL2 and MAGI1 (By similarity). Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen (By similarity). These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B (PubMed:20332120). Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction (PubMed:20332120).
Alternative names
CD144, Cadherin-5, 7B4 antigen, Vascular endothelial cadherin, VE-cadherin, CDH5
Recommended products
Rabbit Recombinant Monoclonal VE Cadherin antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CDH5 aa 1-600.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human CDH5 aa 1-600. The exact immunogen used to generate this antibody is proprietary information. Database link P33151
- Clone number
- BLR091G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 12 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VE Cadherin also known as CD144 or Cadherin-5 is a protein of about 135 kDa involved in cell adhesion. It is a transmembrane protein characterized by its presence at endothelial cell junctions. VE Cadherin belongs to the cadherin superfamily and its expression predominantly occurs in vascular endothelial cells. This protein facilitates the adhesion between adjacent endothelial cells maintaining the integrity of endothelial barriers.
- Biological function summary
VE Cadherin performs essential roles in vascular development and permeability. It is a component of adherens junctions and connects with catenins to mediate cell-cell adhesion through calcium-dependent binding. This protein's adhesive function is critical for blood vessel maturation and response to inflammatory stimuli. Altering VE Cadherin expression impacts vascular stability and endothelial cell layering.
- Pathways
VE Cadherin interacts with the Wnt and VEGF signaling pathways. It plays a role in the regulation of endothelial cell survival and migration. The interaction with β-catenin is important in the Wnt pathway influencing the transcription of target genes that modulate cell behavior. In the VEGF pathway VE Cadherin's association with protein kinase C highlights its involvement in endothelial permeability and angiogenesis processes.
- Associated diseases and disorders
VE Cadherin dysregulation links to cancer progression and atherosclerosis. Its role in maintaining endothelial barrier integrity implicates it in tumor metastasis where disrupted cell adhesion facilitates cancer cell dissemination. Additionally altered VE Cadherin expression or function associates with atherosclerotic disease where damaged vascular endothelium leads to plaque formation. The connection of VE Cadherin with β-catenin in these conditions highlights its significance in pathological angiogenesis and vascular remodeling.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-VE-Cadherin antibody [BLR091G] - BSA free (ab314094), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-blr091g-bsa-free-ab314094--western-blot-img420616.jpg "Western blot - Anti-VE-Cadherin antibody [BLR091G] - BSA free (ab314094)")
Western blot - Anti-VE-Cadherin antibody [BLR091G] - BSA free (ab314094)
Samples: Whole cell lysate from HeLa, HEK293T, BeWo, Jurkat, K-562, HUVEC-C, MCF-7, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-VE-Cadherin recombinant monoclonal antibody [BLR091G] (A700-091) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-COPB2.
All lanes: Western blot - Anti-VE-Cadherin antibody [BLR091G] - BSA free (ab314094) at 1/1000 dilution
Lane 1: HeLa Whole cell lysate at 50 µg
Lane 2: HEK293T Whole cell lysate at 50 µg
Lane 3: BeWo Whole cell lysate at 50 µg
Lane 4: Jurkat Whole cell lysate at 50 µg
Lane 5: K-562 Whole cell lysate at 50 µg
Lane 6: HUVEC-C Whole cell lysate at 50 µg
Lane 7: MCF-7 Whole cell lysate at 50 µg
Lane 8: Hep-G2 Whole cell lysate at 50 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE-Cadherin antibody [BLR091G] - BSA free (ab314094), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-blr091g-bsa-free-ab314094--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420619.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE-Cadherin antibody [BLR091G] - BSA free (ab314094)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE-Cadherin antibody [BLR091G] - BSA free (ab314094)
Detection of human VE-Cadherin in FFPE ovarian carcinoma by immunohistochemistry-IF. Antibody: Rabbit anti-VE-Cadherin recombinant monoclonal antibody [BLR091G] (A700-091). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI.
![Immunocytochemistry - Anti-VE-Cadherin antibody [BLR091G] - BSA free (ab314094), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-blr091g-bsa-free-ab314094--immunocytochemistry-img420620.jpg "Immunocytochemistry - Anti-VE-Cadherin antibody [BLR091G] - BSA free (ab314094)")
Immunocytochemistry - Anti-VE-Cadherin antibody [BLR091G] - BSA free (ab314094)
Detection of human VE-Cadherin in FFPE HUVEC-C cells by ICC. Antibody: Rabbit anti-VE-Cadherin recombinant monoclonal antibody [BLR091G] (A700-091). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE-Cadherin antibody [BLR091G] - BSA free (ab314094), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-blr091g-bsa-free-ab314094--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420618.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE-Cadherin antibody [BLR091G] - BSA free (ab314094)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE-Cadherin antibody [BLR091G] - BSA free (ab314094)
Detection of human VE-Cadherin in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-VE-Cadherin recombinant monoclonal antibody [BLR091G] (A700-091 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
![Immunoprecipitation - Anti-VE-Cadherin antibody [BLR091G] - BSA free (ab314094), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-blr091g-bsa-free-ab314094--immunoprecipitation-img420617.jpg "Immunoprecipitation - Anti-VE-Cadherin antibody [BLR091G] - BSA free (ab314094)")
Immunoprecipitation - Anti-VE-Cadherin antibody [BLR091G] - BSA free (ab314094)
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from BeWo cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-VE-Cadherin recombinant monoclonal antibody [BLR091G] (A700-091) used for IP at 6 µl per reaction. VE-Cadherin was also immunoprecipitated by rabbit anti-VE-Cadherin recombinant monoclonal antibody (BL1361-1E6). For blotting immunoprecipitated VE-Cadherin, A700-091 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.
All lanes: Immunoprecipitation - Anti-VE-Cadherin antibody [BLR091G] - BSA free (ab314094) at 1/1000 dilution
All lanes: BeWo cells at 1 mg
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