AB91064

Anti-VE Cadherin antibody [BV13] - Intercellular Junction Marker

Lab Essentials
Recombinant
What is this?

(1 Review)

(9 Publications)

Rat Recombinant Monoclonal VE Cadherin antibody. Intercellular Junction marker. Suitable for WB, IP, Flow Cyt and reacts with Mouse samples. Cited in 9 publications.

View Alternative Names

CD144, Cadherin-5, Vascular endothelial cadherin, VE-cadherin, Cdh5

4 Images

Flow Cytometry - Anti-VE Cadherin antibody [BV13] - Intercellular Junction Marker (AB91064)

Flow Cyt

Lab

Flow Cytometry - Anti-VE Cadherin antibody [BV13] - Intercellular Junction Marker (AB91064)

Flow cytometric analysis of NIH/3T3 (mouse embryonic fibroblast, Left) / bEnd.3 (mouse brain endothelial cell, Right) cells labelling VE Cadherin with ab91064 at 1/100 dilution (1ug) followed by Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor^® 647, ab150163) secondary at 1/5000 dilution (Red) compared with aRat monoclonal IgG / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) / Blue.

Gated on viable cells.
Negative control : NIH/3T3.

Lab

Immunoprecipitation - Anti-VE Cadherin antibody [BV13] - Intercellular Junction Marker (AB91064)

VE Cadherin was immunoprecipitated from bEnd.3 whole cell lysate with ab91064 at 1/50 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab91064 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-VE Cadherin antibody [BV13] - Intercellular Junction Marker (ab91064) at 1/50 dilution

Lane 1:

bEnd.3 whole cell lysate at 10 µg

Lane 2:

bEnd.3 whole cell lysate

Lane 3:

Rat IgG1- Isotype Control instead of ab91064 in bEnd.3 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/1000 dilution

Predicted band size: 90 kDa,120 kDa

false

Exposure time: 3s

Lab

Western blot - Anti-VE Cadherin antibody [BV13] - Intercellular Junction Marker (AB91064)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.
ab181602 was used as GAPDH loading control at a 1/200000 dilution

Low expression : Mouse brain (PMID : 8555485).

All lanes:

Western blot - Anti-VE Cadherin antibody [BV13] - Intercellular Junction Marker (ab91064) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 40 µg

Lane 2:

Mouse brain tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Predicted band size: 120 kDa

false

Exposure time: 180s

Lab

Western blot - Anti-VE Cadherin antibody [BV13] - Intercellular Junction Marker (AB91064)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.
ab181602 was used as GAPDH loading control at a 1/200000 dilution

Negative control : NIH/3T3 (PMID : 8555485).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 36631513).

All lanes:

Western blot - Anti-VE Cadherin antibody [BV13] - Intercellular Junction Marker (ab91064) at 1/1000 dilution

Lane 1:

bEnd.3 (mouse brain endothelial cell) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Predicted band size: 90 kDa,120 kDa

false

Exposure time: 81s

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

BV13

Isotype

IgG1

Carrier free

Reacts with

Mouse

Applications

WB, Flow Cyt, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "Use 1μg for 106 cells in 100 μl total staining volume.<a href='/en-us/products/unavailable/rat-igg1-kappa-rtk2071-isotype-control-low-endotoxin-azide-free-ab18407'>ab18407</a> - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody." } } }

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Product details

The production method has been switched from hybridoma to recombinant on 24th Oct 2025.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VE Cadherin also known as CD144 or Cadherin-5 is a protein of about 135 kDa involved in cell adhesion. It is a transmembrane protein characterized by its presence at endothelial cell junctions. VE Cadherin belongs to the cadherin superfamily and its expression predominantly occurs in vascular endothelial cells. This protein facilitates the adhesion between adjacent endothelial cells maintaining the integrity of endothelial barriers.

Biological function summary

VE Cadherin performs essential roles in vascular development and permeability. It is a component of adherens junctions and connects with catenins to mediate cell-cell adhesion through calcium-dependent binding. This protein's adhesive function is critical for blood vessel maturation and response to inflammatory stimuli. Altering VE Cadherin expression impacts vascular stability and endothelial cell layering.

Pathways

VE Cadherin interacts with the Wnt and VEGF signaling pathways. It plays a role in the regulation of endothelial cell survival and migration. The interaction with β-catenin is important in the Wnt pathway influencing the transcription of target genes that modulate cell behavior. In the VEGF pathway VE Cadherin's association with protein kinase C highlights its involvement in endothelial permeability and angiogenesis processes.

VE Cadherin dysregulation links to cancer progression and atherosclerosis. Its role in maintaining endothelial barrier integrity implicates it in tumor metastasis where disrupted cell adhesion facilitates cancer cell dissemination. Additionally altered VE Cadherin expression or function associates with atherosclerotic disease where damaged vascular endothelium leads to plaque formation. The connection of VE Cadherin with β-catenin in these conditions highlights its significance in pathological angiogenesis and vascular remodeling.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cadherins are calcium-dependent cell adhesion proteins (By similarity). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types (By similarity). This cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions (PubMed : 20332120, PubMed : 9220534). It associates with alpha-catenin forming a link to the cytoskeleton (By similarity). Plays a role in coupling actin fibers to cell junctions in endothelial cells, via acting as a cell junctional complex anchor for AMOTL2 and MAGI1 (PubMed : 24806444). Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen (PubMed : 27466317). These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B (By similarity). Required for activation of PRKCZ and for localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction (By similarity).

See full target information Cdh5

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Science signaling 16:eabo4863 PubMed37220183

2023

Neuropilin-1 interacts with VE-cadherin and TGFBR2 to stabilize adherens junctions and prevent activation of endothelium under flow.

Applications

Unspecified application

Species

Unspecified reactive species

Emy Bosseboeuf,Anissa Chikh,Ahmed Bey Chaker,Tom P Mitchell,Dhilakshani Vignaraja,Ridhi Rajendrakumar,Rayomand S Khambata,Thomas D Nightingale,Justin C Mason,Anna M Randi,Amrita Ahluwalia,Claudio Raimondi

Protein & cell 13:842-862 PubMed35394262

2022

Postnatal state transition of cardiomyocyte as a primary step in heart maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Li,Fang Yao,Peng Yu,Dandan Li,Mingzhi Zhang,Lin Mao,Xiaomeng Shen,Zongna Ren,Li Wang,Bingying Zhou

Nature cancer 2:545-562 PubMed35122017

2021

Neutrophil oxidative stress mediates obesity-associated vascular dysfunction and metastatic transmigration.

Applications

Unspecified application

Species

Unspecified reactive species

Sheri A C McDowell,Robin B E Luo,Azadeh Arabzadeh,Samuel Doré,Nicolas C Bennett,Valérie Breton,Elham Karimi,Morteza Rezanejad,Ryan R Yang,Katherine D Lach,Marianne S M Issac,Bozena Samborska,Lucas J M Perus,Dan Moldoveanu,Yuhong Wei,Benoit Fiset,Roni F Rayes,Ian R Watson,Lawrence Kazak,Marie-Christine Guiot,Pierre O Fiset,Jonathan D Spicer,Andrew J Dannenberg,Logan A Walsh,Daniela F Quail

Clinical science (London, England : 1979) 135:829-846 PubMed33720353

2021

SOX7 suppresses endothelial-to-mesenchymal transitions by enhancing VE-cadherin expression during outflow tract development.

Applications

Unspecified application

Species

Unspecified reactive species

Xuechao Jiang,Tingting Li,Bojian Li,Wei Wei,Fen Li,Sun Chen,Rang Xu,Kun Sun

ACS nano 14:12732-12748 PubMed32931251

2020

Exosome-Mediated Crosstalk between Keratinocytes and Macrophages in Cutaneous Wound Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoju Zhou,Brooke A Brown,Amanda P Siegel,Mohamed S El Masry,Xuyao Zeng,Woran Song,Amitava Das,Puneet Khandelwal,Andrew Clark,Kanhaiya Singh,Poornachander R Guda,Mahadeo Gorain,Lava Timsina,Yi Xuan,Stephen C Jacobson,Milos V Novotny,Sashwati Roy,Mangilal Agarwal,Robert J Lee,Chandan K Sen,David E Clemmer,Subhadip Ghatak

Journal of cachexia, sarcopenia and muscle 8:954-973 PubMed29130633

2017

The mitochondrial metabolic reprogramming agent trimetazidine as an 'exercise mimetic' in cachectic C26-bearing mice.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Molinari,Fabrizio Pin,Stefania Gorini,Sergio Chiandotto,Laura Pontecorvo,Fabio Penna,Emanuele Rizzuto,Simona Pisu,Antonio Musarò,Paola Costelli,Giuseppe Rosano,Elisabetta Ferraro

The Journal of cell biology 211:1177-92 PubMed26668327

2015

The actin-binding protein EPS8 binds VE-cadherin and modulates YAP localization and signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Costanza Giampietro,Andrea Disanza,Luca Bravi,Miriam Barrios-Rodiles,Monica Corada,Emanuela Frittoli,Cecilia Savorani,Maria Grazia Lampugnani,Barbara Boggetti,Carien Niessen,Jeff L Wrana,Giorgio Scita,Elisabetta Dejana

Nature 502:105-9 PubMed23995678

2013

Podoplanin maintains high endothelial venule integrity by interacting with platelet CLEC-2.

Applications

Unspecified application

Species

Unspecified reactive species

Brett H Herzog,Jianxin Fu,Stephen J Wilson,Paul R Hess,Aslihan Sen,J Michael McDaniel,Yanfang Pan,Minjia Sheng,Tadayuki Yago,Robert Silasi-Mansat,Samuel McGee,Frauke May,Bernhard Nieswandt,Andrew J Morris,Florea Lupu,Shaun R Coughlin,Rodger P McEver,Hong Chen,Mark L Kahn,Lijun Xia

The Journal of experimental medicine 208:2393-401 PubMed22025303

2011

Dissociation of VE-PTP from VE-cadherin is required for leukocyte extravasation and for VEGF-induced vascular permeability in vivo.

Applications

WB, ICC/IF

Species

Mouse, Mouse

Andre Broermann,Mark Winderlich,Helena Block,Maike Frye,Jan Rossaint,Alexander Zarbock,Giuseppe Cagna,Ruth Linnepe,Dörte Schulte,Astrid Fee Nottebaum,Dietmar Vestweber

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VE Cadherin antibody [BV13] - Intercellular Junction Marker

Flow Cytometry - Anti-VE Cadherin antibody [BV13] - Intercellular Junction Marker (AB91064)

Immunoprecipitation - Anti-VE Cadherin antibody [BV13] - Intercellular Junction Marker (AB91064)

Western blot - Anti-VE Cadherin antibody [BV13] - Intercellular Junction Marker (AB91064)

Western blot - Anti-VE Cadherin antibody [BV13] - Intercellular Junction Marker (AB91064)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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