Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (AB232515)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized bEnd.3 (Mouse brain microvascular endothelial cell line) cells labeling VE Cadherin with ab205336 at 1/1000 dilution, followed by Goat anti-rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

Confocal image showing membrane staining on bEnd.3 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody -Loading control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (AlexaFluor®594 ) preadsorbed (ab150120) at 1/500 dilution (red).

The negative controls are as follows : -

-ve control 1 : ab205336 at 1/1000 dilution followed by ab150120 at 1/500 dilution.

-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205336).

• IP

Supplier Data

Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (AB232515)

VE Cadherin was immunoprecipitated from 1mg of bEnd.3 (Mouse brain microvascular endothelial cell line) whole cell lysate with ab205336 at 1/80 dilution.

Western blot was performed from the immunoprecipitate using ab205336 at 1/1000 dilution.

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

Lane 1 : bEnd.3 whole cell lysate 10ug (Input).

Lane 2 : ab205336 IP in bEnd.3 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab205336 in bEnd.3 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205336).

All lanes:

Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (<a href='/en-us/products/primary-antibodies/ve-cadherin-antibody-epr18229-intercellular-junction-marker-ab205336'>ab205336</a>)

Predicted band size: 88 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (AB232515)

VE Cadherin was immunoprecipitated from 1mg of Mouse lung whole cell lysate with ab205336 at 1/80 dilution.

Western blot was performed from the immunoprecipitate using ab205336 at 1/1000 dilution.

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

Lane 1 : Mouse lung whole cell lysate 10ug (Input).

Lane 2 : ab205336 IP in Mouse lung whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab205336 in Mouse lung whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

Due to a high degree of glycosylation and phosphorylation, the observed MW is higher than the predicted MW. The 90kDa fragment represents the extracellular domain where the immunogen is located.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205336).

All lanes:

Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (<a href='/en-us/products/primary-antibodies/ve-cadherin-antibody-epr18229-intercellular-junction-marker-ab205336'>ab205336</a>)

Predicted band size: 88 kDa

false