Rabbit Monoclonal VE Cadherin antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Mouse samples. Cited in 1 publication.
Images
![Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (AB232515), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-epr18229-bsa-and-azide-free-ab232515--immunoprecipitation-img9337.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (AB232515)")
![Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (AB232515), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-epr18229-bsa-and-azide-free-ab232515--immunoprecipitation-img21888.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (AB232515)")
![Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (AB232515), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-epr18229-bsa-and-azide-free-ab232515--immunocytochemistry-immunofluorescence-img93860.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (AB232515)")
Publications
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- Frontiers in pharmacology 12:7590272022QiShenYiQi Inhibits Tissue Plasminogen Activator-Induced Brain Edema and Hemorrhage after Ischemic Stroke in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Ye,Quan Li,Chun-Shui Pan,Li Yan,Kai Sun,Xiao-Yi Wang,Shu-Qi Yao,Jing-Yu Fan,Jing-Yan HanPubMed 35095486
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Mouse | Tested | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
2 products for Alternative Version
Target data
Function
Cadherins are calcium-dependent cell adhesion proteins (By similarity). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types (By similarity). This cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions (PubMed:20332120, PubMed:9220534). It associates with alpha-catenin forming a link to the cytoskeleton (By similarity). Plays a role in coupling actin fibers to cell junctions in endothelial cells, via acting as a cell junctional complex anchor for AMOTL2 and MAGI1 (PubMed:24806444). Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen (PubMed:27466317). These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B (By similarity). Required for activation of PRKCZ and for localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction (By similarity).
Alternative names
CD144, Cadherin-5, Vascular endothelial cadherin, VE-cadherin, Cdh5
Recommended products
Rabbit Monoclonal VE Cadherin antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Mouse samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR18229
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab232515 is the carrier-free version of Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker ab205336.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VE Cadherin also known as CD144 or Cadherin-5 is a protein of about 135 kDa involved in cell adhesion. It is a transmembrane protein characterized by its presence at endothelial cell junctions. VE Cadherin belongs to the cadherin superfamily and its expression predominantly occurs in vascular endothelial cells. This protein facilitates the adhesion between adjacent endothelial cells maintaining the integrity of endothelial barriers.
- Biological function summary
VE Cadherin performs essential roles in vascular development and permeability. It is a component of adherens junctions and connects with catenins to mediate cell-cell adhesion through calcium-dependent binding. This protein's adhesive function is critical for blood vessel maturation and response to inflammatory stimuli. Altering VE Cadherin expression impacts vascular stability and endothelial cell layering.
- Pathways
VE Cadherin interacts with the Wnt and VEGF signaling pathways. It plays a role in the regulation of endothelial cell survival and migration. The interaction with β-catenin is important in the Wnt pathway influencing the transcription of target genes that modulate cell behavior. In the VEGF pathway VE Cadherin's association with protein kinase C highlights its involvement in endothelial permeability and angiogenesis processes.
- Associated diseases and disorders
VE Cadherin dysregulation links to cancer progression and atherosclerosis. Its role in maintaining endothelial barrier integrity implicates it in tumor metastasis where disrupted cell adhesion facilitates cancer cell dissemination. Additionally altered VE Cadherin expression or function associates with atherosclerotic disease where damaged vascular endothelium leads to plaque formation. The connection of VE Cadherin with β-catenin in these conditions highlights its significance in pathological angiogenesis and vascular remodeling.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (ab232515), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-epr18229-bsa-and-azide-free-ab232515--immunoprecipitation-img9337.jpg "Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (ab232515)")
Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (ab232515)
VE Cadherin was immunoprecipitated from 1mg of Mouse lung whole cell lysate with Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker ab205336 at 1/80 dilution.
Western blot was performed from the immunoprecipitate using Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker ab205336 at 1/1000 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: Mouse lung whole cell lysate 10ug (Input).
Lane 2: Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker ab205336 IP in Mouse lung whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker ab205336 in Mouse lung whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Due to a high degree of glycosylation and phosphorylation, the observed MW is higher than the predicted MW. The 90kDa fragment represents the extracellular domain where the immunogen is located.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker ab205336).
All lanes: Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker ab205336)
Predicted band size: 88 kDa
![Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (ab232515), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-epr18229-bsa-and-azide-free-ab232515--immunoprecipitation-img21888.jpg "Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (ab232515)")
Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (ab232515)
VE Cadherin was immunoprecipitated from 1mg of bEnd.3 (Mouse brain microvascular endothelial cell line) whole cell lysate with Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker ab205336 at 1/80 dilution.
Western blot was performed from the immunoprecipitate using Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker ab205336 at 1/1000 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: bEnd.3 whole cell lysate 10ug (Input).
Lane 2: Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker ab205336 IP in bEnd.3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker ab205336 in bEnd.3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker ab205336).
All lanes: Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker ab205336)
Predicted band size: 88 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (ab232515), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-epr18229-bsa-and-azide-free-ab232515--immunocytochemistry-immunofluorescence-img93860.jpg "Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (ab232515)")
Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (ab232515)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized bEnd.3 (Mouse brain microvascular endothelial cell line) cells labeling VE Cadherin with Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker ab205336 at 1/1000 dilution, followed by Goat anti-rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green).
Confocal image showing membrane staining on bEnd.3 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody -Loading control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (AlexaFluor®594 ) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1: Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker ab205336 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker ab205336).
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