Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336) is a rabbit monoclonal antibody that is used to detect VE Cadherin in Western Blot, IP, ICC/IF. Suitable for Mouse samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 40 publications
Images
![Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (AB205336), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-epr18229-intercellular-junction-marker-ab205336--immunocytochemistry-immunofluorescence-img93859.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (AB205336)")
![Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (AB205336), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-epr18229-intercellular-junction-marker-ab205336--immunoprecipitation-img9337.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (AB205336)")
![Western blot - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (AB205336), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-epr18229-intercellular-junction-marker-ab205336--western-blot-img145379.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (AB205336)")
![Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (AB205336), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-epr18229-intercellular-junction-marker-ab205336--immunoprecipitation-img21888.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (AB205336)")
![Western blot - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (AB205336), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-epr18229-intercellular-junction-marker-ab205336--western-blot-img54719.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (AB205336)")
Publications
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- JCI insight 9:2024Efficient pulmonary lymphatic drainage is necessary for inflammation resolution in ARDS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPu-Hong Zhang,Wen-Wu Zhang,Shun-Shun Wang,Cheng-Hua Wu,Yang-Dong Ding,Xin-Yi Wu,Fang Gao Smith,Yu Hao,Sheng-Wei JinPubMed 37971881
- World journal of stem cells 15:1017-10342023Dissecting molecular mechanisms underlying ferroptosis in human umbilical cord mesenchymal stem cells: Role of cystathionine γ-lyase/hydrogen sulfide pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Hu,Xiang-Xi Zhang,Tao Zhang,Wan-Cheng YuPubMed 38058959
- Journal of inflammation research 16:5095-51092023LPA Alleviates Septic Acute Lung Injury via Protective Endothelial Barrier Function Through Activation of PLC-PKC-FAK.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuifeng Bai,Jianqiu Pei,Shengqiang Pei,Xiangfeng Cong,Jerold Chun,Fang Wang,Xi ChenPubMed 38026263
- Journal of neuroinflammation 20:2442023ASK1-K716R reduces neuroinflammation and white matter injury via preserving blood-brain barrier integrity after traumatic brain injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShan Meng,Hui Cao,Yichen Huang,Ziyu Shi,Jiaying Li,Yana Wang,Yue Zhang,Shuning Chen,Hong Shi,Yanqin GaoPubMed 37875988
- Journal of neuroinflammation 20:2222023Inhibition of neutrophil extracellular trap formation ameliorates neuroinflammation and neuronal apoptosis via STING-dependent IRE1α/ASK1/JNK signaling pathway in mice with traumatic brain injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuihong Shi,Liang Liu,Yiyao Cao,Guangshuo Ma,Yanlin Zhu,Jianye Xu,Xu Zhang,Tuo Li,Liang Mi,Haoran Jia,Yanfeng Zhang,Xilei Liu,Yuan Zhou,Shenghui Li,Guili Yang,Xiao Liu,Fanglian Chen,Baolong Wang,Quanjun Deng,Shu Zhang,Jianning ZhangPubMed 37777772
- Journal of vascular research 60:234-2442023Isolation and Cultivation of Vascular Smooth Muscle Cells from the Mouse Circle of Willis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Chang,Yajuan Li,Fengzhou Liu,Kehai Zang,Peiran Zhang,Shuai Qu,Jingyu Zhao,Junhui XuePubMed 37643584
- Cardiovascular diabetology 22:2162023Endothelial MICU1 alleviates diabetic cardiomyopathy by attenuating nitrative stress-mediated cardiac microvascular injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXide Shi,Chao Liu,Jiangwei Chen,Shiqiang Zhou,Yajuan Li,Xingcheng Zhao,Jinliang Xing,Junhui Xue,Fengzhou Liu,Fei LiPubMed 37592255
- Nature communications 14:49652023Astroglial Hmgb1 regulates postnatal astrocyte morphogenesis and cerebrovascular maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMoises Freitas-Andrade,Cesar H Comin,Peter Van Dyken,Julie Ouellette,Joanna Raman-Nair,Nicole Blakeley,Qing Yan Liu,Sonia Leclerc,Youlian Pan,Ziying Liu,Micaël Carrier,Karan Thakur,Alexandre Savard,Gareth M Rurak,Marie-Ève Tremblay,Natalina Salmaso,Luciano da F Costa,Gianfilippo Coppola,Baptiste LacostePubMed 37587100
- Journal of neuroinflammation 20:1272023Lung infection by Pseudomonas aeruginosa induces neuroinflammation and blood-brain barrier dysfunction in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNuria Villalba,Yonggang Ma,Sarah A Gahan,Aurelie Joly-Amado,Sam Spence,Xiaoyuan Yang,Kevin R Nash,Sarah Y YuanPubMed 37245027
- ACS nano 17:7562-75752023Extracellular Vesicle ITGAM and ITGB2 Mediate Severe Acute Pancreatitis-Related Acute Lung Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Hu,Shu Zhang,Yue Yang,Juan Li,Hongxin Kang,Wenfu Tang,Christopher J Lyon,Meihua WanPubMed 37022097
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Mouse | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/80 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Cadherins are calcium-dependent cell adhesion proteins (By similarity). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types (By similarity). This cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions (PubMed:20332120, PubMed:9220534). It associates with alpha-catenin forming a link to the cytoskeleton (By similarity). Plays a role in coupling actin fibers to cell junctions in endothelial cells, via acting as a cell junctional complex anchor for AMOTL2 and MAGI1 (PubMed:24806444). Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen (PubMed:27466317). These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B (By similarity). Required for activation of PRKCZ and for localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction (By similarity).
Alternative names
CD144, Cadherin-5, Vascular endothelial cadherin, VE-cadherin, Cdh5
Recommended products
Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336) is a rabbit monoclonal antibody that is used to detect VE Cadherin in Western Blot, IP, ICC/IF. Suitable for Mouse samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 40 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR18229
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.
What is the molecular weight of VE Cadherin?
Anti-VE Cadherin [EPR18229] - Intercellular Junction Marker (ab205336) specifically detects a band for VE Cadherin (UniProt: P55284) at a molecular weight of 88kDa.
Trusted by the scientific community
Anti-VE Cadherin [EPR18229] - Intercellular Junction Marker (ab205336) was first used in a scientific publication in 2015 and has been cited over 40 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR18229] also available for your convenience:
ab205336, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker ab225442, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker ab225443, Carrier free - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free ab232515, Carrier free - Anti-VE Cadherin antibody [EPR18229] - BSA and Azide free (Capture) ab242594
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VE Cadherin also known as CD144 or Cadherin-5 is a protein of about 135 kDa involved in cell adhesion. It is a transmembrane protein characterized by its presence at endothelial cell junctions. VE Cadherin belongs to the cadherin superfamily and its expression predominantly occurs in vascular endothelial cells. This protein facilitates the adhesion between adjacent endothelial cells maintaining the integrity of endothelial barriers.
- Biological function summary
VE Cadherin performs essential roles in vascular development and permeability. It is a component of adherens junctions and connects with catenins to mediate cell-cell adhesion through calcium-dependent binding. This protein's adhesive function is critical for blood vessel maturation and response to inflammatory stimuli. Altering VE Cadherin expression impacts vascular stability and endothelial cell layering.
- Pathways
VE Cadherin interacts with the Wnt and VEGF signaling pathways. It plays a role in the regulation of endothelial cell survival and migration. The interaction with Β-catenin is important in the Wnt pathway influencing the transcription of target genes that modulate cell behavior. In the VEGF pathway VE Cadherin's association with protein kinase C highlights its involvement in endothelial permeability and angiogenesis processes.
- Associated diseases and disorders
VE Cadherin dysregulation links to cancer progression and atherosclerosis. Its role in maintaining endothelial barrier integrity implicates it in tumor metastasis where disrupted cell adhesion facilitates cancer cell dissemination. Additionally altered VE Cadherin expression or function associates with atherosclerotic disease where damaged vascular endothelium leads to plaque formation. The connection of VE Cadherin with Β-catenin in these conditions highlights its significance in pathological angiogenesis and vascular remodeling.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-epr18229-intercellular-junction-marker-ab205336--immunocytochemistry-immunofluorescence-img93859.jpg "Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)")
Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized bEnd.3 (Mouse brain microvascular endothelial cell line) cells labeling VE Cadherin with ab205336 at 1/1000 dilution, followed by Goat anti-rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green).
Confocal image showing membrane staining on bEnd.3 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody -Loading control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (AlexaFluor®594 ) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1: ab205336 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/500 dilution.
![Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-epr18229-intercellular-junction-marker-ab205336--immunoprecipitation-img9337.jpg "Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)")
Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
VE Cadherin was immunoprecipitated from 1mg of Mouse lung whole cell lysate with ab205336 at 1/80 dilution.
Western blot was performed from the immunoprecipitate using ab205336 at 1/1000 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: Mouse lung whole cell lysate 10ug (Input).
Lane 2: ab205336 IP in Mouse lung whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab205336 in Mouse lung whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Due to a high degree of glycosylation and phosphorylation, the observed MW is higher than the predicted MW. The 90kDa fragment represents the extracellular domain where the immunogen is located.
All lanes: Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
Predicted band size: 88 kDa
![Western blot - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-epr18229-intercellular-junction-marker-ab205336--western-blot-img145379.jpg "Western blot - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)")
Western blot - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
Blocking/Dilution buffer: 5% NFDM/TBST.
Due to a high degree of glycosylation and phosphorylation, the observed MW is higher than the predicted MW. The 90kDa fragment represents the extracellular domain where the immunogen is located.
All lanes: Western blot - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336) at 1/1000 dilution
Lane 1: Mouse lung lysate at 20 µg
Lane 2: Mouse placenta lysate at 20 µg
Lane 3: bEnd.3 (Mouse brain microvascular endothelial cell line) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 88 kDa
Observed band size: 125 kDa, 90 kDa
Exposure time: 1s
![Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-epr18229-intercellular-junction-marker-ab205336--immunoprecipitation-img21888.jpg "Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)")
Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
VE Cadherin was immunoprecipitated from 1mg of bEnd.3 (Mouse brain microvascular endothelial cell line) whole cell lysate with ab205336 at 1/80 dilution.
Western blot was performed from the immunoprecipitate using ab205336 at 1/1000 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: bEnd.3 whole cell lysate 10ug (Input).
Lane 2: ab205336 IP in bEnd.3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab205336 in bEnd.3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
All lanes: Immunoprecipitation - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
Predicted band size: 88 kDa
![Western blot - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336), expandable thumbnail](https://content.abcam.com/products/images/ve-cadherin-antibody-epr18229-intercellular-junction-marker-ab205336--western-blot-img54719.jpg "Western blot - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)")
Western blot - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336)
Blocking/Dilution buffer: 5% NFDM/TBST.
Due to a high degree of glycosylation and phosphorylation, the observed MW is higher than the predicted MW. The 90kDa fragment represents the extracellular domain where the immunogen is located.
All lanes: Western blot - Anti-VE Cadherin antibody [EPR18229] - Intercellular Junction Marker (ab205336) at 1/1000 dilution
Lane 1: Mouse heart lysate at 10 µg
Lane 2: Mouse kidney lysate at 10 µg
Lane 3: Mouse spleen lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 88 kDa
Observed band size: 120 kDa, 90 kDa
Exposure time: 1min
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