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Rabbit Recombinant Monoclonal VE Cadherin antibody. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra), WB, IP and reacts with Human samples.

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Images

Immunoprecipitation - Anti-VE Cadherin antibody [EPR27436-55] (AB313632), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody [EPR27436-55] (AB313632), expandable thumbnail
  • Western blot - Anti-VE Cadherin antibody [EPR27436-55] (AB313632), expandable thumbnail
  • Western blot - Anti-VE Cadherin antibody [EPR27436-55] (AB313632), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-VE Cadherin antibody [EPR27436-55] (AB313632), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFFlow Cyt (Intra)WBIP
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Cadherins are calcium-dependent cell adhesion proteins (By similarity). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types (PubMed:21269602). This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions (By similarity). It associates with alpha-catenin forming a link to the cytoskeleton (PubMed:10861224). Plays a role in coupling actin fibers to cell junctions in endothelial cells, via acting as a cell junctional complex anchor for AMOTL2 and MAGI1 (By similarity). Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen (By similarity). These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B (PubMed:20332120). Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction (PubMed:20332120).

Alternative names

CD144, Cadherin-5, 7B4 antigen, Vascular endothelial cadherin, VE-cadherin, CDH5

Recommended products

Rabbit Recombinant Monoclonal VE Cadherin antibody. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra), WB, IP and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27436-55
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

VE Cadherin also known as CD144 or Cadherin-5 is a protein of about 135 kDa involved in cell adhesion. It is a transmembrane protein characterized by its presence at endothelial cell junctions. VE Cadherin belongs to the cadherin superfamily and its expression predominantly occurs in vascular endothelial cells. This protein facilitates the adhesion between adjacent endothelial cells maintaining the integrity of endothelial barriers.

Biological function summary

VE Cadherin performs essential roles in vascular development and permeability. It is a component of adherens junctions and connects with catenins to mediate cell-cell adhesion through calcium-dependent binding. This protein's adhesive function is critical for blood vessel maturation and response to inflammatory stimuli. Altering VE Cadherin expression impacts vascular stability and endothelial cell layering.

Pathways

VE Cadherin interacts with the Wnt and VEGF signaling pathways. It plays a role in the regulation of endothelial cell survival and migration. The interaction with β-catenin is important in the Wnt pathway influencing the transcription of target genes that modulate cell behavior. In the VEGF pathway VE Cadherin's association with protein kinase C highlights its involvement in endothelial permeability and angiogenesis processes.

Associated diseases and disorders

VE Cadherin dysregulation links to cancer progression and atherosclerosis. Its role in maintaining endothelial barrier integrity implicates it in tumor metastasis where disrupted cell adhesion facilitates cancer cell dissemination. Additionally altered VE Cadherin expression or function associates with atherosclerotic disease where damaged vascular endothelium leads to plaque formation. The connection of VE Cadherin with β-catenin in these conditions highlights its significance in pathological angiogenesis and vascular remodeling.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunoprecipitation - Anti-VE Cadherin antibody [EPR27436-55] (ab313632), expandable thumbnail

    Immunoprecipitation - Anti-VE Cadherin antibody [EPR27436-55] (ab313632)

    VE Cadherin was immunoprecipitated from 0.35 mg HUVEC (human umbilical vein endothelial cell) whole cell lysate with ab313632 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313632 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: HUVEC (human umbilical vein endothelial cell) whole cell lysate
    Lane 2: ab313632 IP in HUVEC (human umbilical vein endothelial cell) whole cell lysate
    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab313632 in HUVEC whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 seconds
    The molecular weight observed is consistent with what has been described in the literature (PMID: 36631513).

    All lanes: Immunoprecipitation - Anti-VE Cadherin antibody [EPR27436-55] (ab313632) at 1/30 dilution

    All lanes: HUVEC (human umbilical vein endothelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Exposure time: 3s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody [EPR27436-55] (ab313632), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody [EPR27436-55] (ab313632)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling VE Cadherin with ab313632 at 1/2000 (0.258 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on vascular endothelium of human kidney (PMID: 29846473). The section was incubated with ab313632 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-VE Cadherin antibody [EPR27436-55] (ab313632), expandable thumbnail

    Western blot - Anti-VE Cadherin antibody [EPR27436-55] (ab313632)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: Hela (PMID: 35164755)

    Samples are non-boiled as boiling may cause protein aggregation.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 36631513).

    In Western blot, anti- H3 antibody (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) loading control staining at 1/100000 dilution.
    Exposure time: 3 seconds

    All lanes: Western blot - Anti-VE Cadherin antibody [EPR27436-55] (ab313632) at 1/1000 dilution

    Lane 1: HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

    Lane 2: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 87-100, 130 kDa

    Exposure time: 3s

  • Western blot - Anti-VE Cadherin antibody [EPR27436-55] (ab313632), expandable thumbnail

    Western blot - Anti-VE Cadherin antibody [EPR27436-55] (ab313632)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Samples are non-boiled as boiling may cause protein aggregation.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 36631513).
    Exposure time: 3 seconds

    All lanes: Western blot - Anti-VE Cadherin antibody [EPR27436-55] (ab313632) at 1/1000 dilution

    Lane 1: Human lu tissue lysate at 20 µg

    Lane 2: Human kidney tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 87-100, 130 kDa

    Exposure time: 3s

  • Flow Cytometry (Intracellular) - Anti-VE Cadherin antibody [EPR27436-55] (ab313632), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-VE Cadherin antibody [EPR27436-55] (ab313632)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell, Left) / HUVEC (human umbilical vein endothelial cell, Right) cells labelling VE Cadherin with ab313632 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Negative control: HeLa (PMID: 35164755).

  • Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody [EPR27436-55] (ab313632), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody [EPR27436-55] (ab313632)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized HUVEC (human umbilical vein endothelial cell) cells labelling VE Cadherin with ab313632 at 1/50 (10.32 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous and cytoplasmic staining in HUVEC cell line.Negative control: HeLa (PMID: 35164755).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody [EPR27436-55] (ab313632), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody [EPR27436-55] (ab313632)

    Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling VE Cadherin with ab313632 at 1/2000 (0.258 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on vascular endothelium of human colon cancer (PMID: 27466381). The section was incubated with ab313632 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody [EPR27436-55] (ab313632), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VE Cadherin antibody [EPR27436-55] (ab313632)

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling VE Cadherin with ab313632 at 1/2000 (0.258 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on human lung (PMID: 29846473). The section was incubated with ab313632 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

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