Anti-VE Cadherin antibody ab33168 is a rabbit polyclonal antibody that is used in VE Cadherin western blotting and immunofluorescence. Suitable for human and mouse samples.
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- BMC biology 21:2902023Biomechanical stimulation promotes blood vessel growth despite VEGFR-2 inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBronte Miller Johnson,Allison McKenzie Johnson,Michael Heim,Molly Buckley,Bryan Mortimer,Joel L Berry,Mary Kathryn Sewell-LoftinPubMed 38072992
- Oncology reports 51:2023Unravelling heterogeneous effects of cancer‑associated fibroblasts on poor prognosis markers in breast cancer EM‑G3 cell line: ‑targeted treatment (anti‑IL-6, anti‑VEGF-A, anti‑MFGE8) based on transcriptomic profiling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLukáš Urban,Štepán Novák,Matúš Čoma,Barbora Dvořánková,Lukáš Lacina,Jana Šáchová,Miluše Hradilová,Petra Svatoňová,Michal Kolář,Hynek Strnad,Jana Březinová,Karel Smetana,Peter Gál,Pavol SzaboPubMed 37975220
- Respiratory research 24:2882023Deficiency of S100A8/A9 attenuates pulmonary microvascular leakage in septic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiang Yu,Boying Zhao,Qiangzhong Pi,Guoxiang Zhou,Zhe Cheng,Can Qu,Xiaowen Wang,Lingwen Kong,Suxin Luo,Dingyuan Du,Yongzheng GuoPubMed 37978525
- International journal of molecular sciences 24:2023NLRP3 Inflammasome Activates Endothelial-to-Mesenchymal Transition via Focal Adhesion Kinase Pathway in Bleomycin-Induced Pulmonary Fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei-Chih Chen,Wen-Kuang Yu,Vincent Yi-Fong Su,Han-Shui Hsu,Kuang-Yao YangPubMed 37958797
- Biomedical microdevices 25:402023Role of interleukin 6 and its soluble receptor on the diffusion barrier dysfunction of alveolar tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Sui,Woo LeePubMed 37851124
- Frontiers in molecular neuroscience 16:12501232023Human BBB-on-a-chip reveals barrier disruption, endothelial inflammation, and T cell migration under neuroinflammatory conditions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArya Lekshmi Nair,Linda Groenendijk,Roos Overdevest,Tania M Fowke,Rumaisha Annida,Orsola Mocellin,Helga E de Vries,Nienke R WeversPubMed 37818458
- Clinical and translational medicine 13:e13772023SIRT6 deficiency in endothelial cells exacerbates oxidative stress by enhancing HIF1α accumulation and H3K9 acetylation at the Ero1α promoter.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenyang Guo,Xueting Yu,Shuang Zhao,Xin Zhong,Dong Huang,Runyang Feng,Peng Li,Zheyan Fang,Yiqing Hu,Zhentao Zhang,Mukaddas Abdurahman,Lei Huang,Yun Zhao,Xiangdong Wang,Junbo Ge,Hua LiPubMed 37598403
- International journal of molecular sciences 24:2023GV1001 Inhibits the Severity of the Ligature-Induced Periodontitis and the Vascular Lipid Deposition Associated with the Periodontitis in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSharon Y Kim,Yun-Jeong Kim,Suyang Kim,Mersedeh Momeni,Alicia Lee,Alexandra Treanor,Sangjae Kim,Reuben H Kim,No-Hee ParkPubMed 37628753
- Cell communication and signaling : CCS 21:1762023Neutrophil extracellular traps promote angiogenesis in gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShifeng Yang,Boshi Sun,Jiacheng Li,Nana Li,Ange Zhang,Xinyu Zhang,Hao Yang,Xiaoming ZouPubMed 37480055
- Nature communications 14:42692023Engineering tumoral vascular leakiness with gold nanoparticles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMagdiel Inggrid Setyawati,Qin Wang,Nengyi Ni,Jie Kai Tee,Katsuhiko Ariga,Pu Chun Ke,Han Kiat Ho,Yucai Wang,David Tai LeongPubMed 37460554
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Chicken | Predicted | Predicted |
| Pig | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Abcam recommends using BSA blocking with this product. Milk blocking will give a greatly reduced signal strength in WB. |
Species Mouse | Dilution info 1 µg/mL | Notes Abcam recommends using BSA blocking with this product. Milk blocking will give a greatly reduced signal strength in WB. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.1-1 µg/mL | Notes Abcam recommends using this product with confluent cells. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Pig | Dilution info - | Notes - |
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Target data
Function
Cadherins are calcium-dependent cell adhesion proteins (By similarity). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types (PubMed:21269602). This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions (By similarity). It associates with alpha-catenin forming a link to the cytoskeleton (PubMed:10861224). Plays a role in coupling actin fibers to cell junctions in endothelial cells, via acting as a cell junctional complex anchor for AMOTL2 and MAGI1 (By similarity). Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen (By similarity). These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B (PubMed:20332120). Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction (PubMed:20332120).
Alternative names
CD144, Cadherin-5, 7B4 antigen, Vascular endothelial cadherin, VE-cadherin, CDH5
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Anti-VE Cadherin antibody ab33168 is a rabbit polyclonal antibody that is used in VE Cadherin western blotting and immunofluorescence. Suitable for human and mouse samples.
- Tried and trusted by researchers since 2007
Same trusted quality, new lower price
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
From May 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168) is a rabbit polyclonal antibody and is validated for use in ICC/IF, WB in mouse, rat samples.
Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168) has been cited over 361 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168) has high sensitivity and specificity.
Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168) has 39 independent reviews from customers.
Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168) specifically detects VE Cadherin (UniProt ID: P33151; Molecular weight: 83kDa) and is sold in 100 µg selling sizes.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VE Cadherin also known as Cadherin-5 or CD144 is a type of protein with a mass of approximately 120 kDa. It is mainly found in endothelial cells where it plays an important role in maintaining endothelial integrity and stability. As a member of the cadherin family VE Cadherin facilitates cell-cell adhesion by interacting with other cadherins on adjacent cells through its extracellular domain. This protein helps form adherens junctions important structures that stabilize cell layers and maintain tissue architecture.
- Biological function summary
VE Cadherin functions as an important component in maintaining vascular integrity. It connects to cytoskeletal elements through catenins forming a complex that transmits mechanical signals to maintain junctional stability. This ability is integral to the control of vascular permeability and the regulation of endothelial cell movement and growth. The anchoring of VE Cadherin to the actin cytoskeleton ensures that endothelial cells remain tightly bound limiting permeability and preventing unwarranted fluid leakage.
- Pathways
VE Cadherin participates in the Wnt/?-catenin pathway and the Rho GTPase signal transduction. In the Wnt/?-catenin pathway VE Cadherin interacts with ?-catenin playing a pivotal role in mediating cell signaling that governs cell proliferation and differentiation. In the Rho GTPase pathway VE Cadherin influences cytoskeletal dynamics and cell morphology through interactions with small GTPases such as RhoA. These pathways highlight VE Cadherin's involvement in cellular processes including cell movement and stability.
- Associated diseases and disorders
VE Cadherin has associations with cancer progression and cardiovascular diseases. In cancer alterations in VE Cadherin expression can lead to increased tumor angiogenesis and metastasis due to disrupted cell adhesion. In cardiovascular contexts abnormalities in VE Cadherin contribute to vascular permeability changes seen in conditions like atherosclerosis. Proteins such as ?-catenin and p120-catenin which partner with VE Cadherin also play roles in these pathological processes further emphasizing its importance in disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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9 product images
Immunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
VE Cadherin immunofluorescence staining of HUVEC cells using rabbit anti-VE Cadherin antibody
ab33168 staining VE Cadherin in HUV-EC cells. The cells were fixed with 100% methanol (5 min) permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab33168 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).Image was acquired with a high-content analyser (Operetta CLS Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab33168 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
The band we observe at 115 kDa is believed to be the glycosylated form of the protein.
All lanes: Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168) at 1 µg/mL
All lanes: HUVEC Cell Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 88 kDa
Observed band size: 120 kDa
Exposure time: 1min
Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab33168 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
The band we observe at 115 kDa is believed to be the glycosylated form of the protein.
All lanes: Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168) at 1 µg/mL
All lanes: HUVEC Cell Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 88 kDa
Observed band size: 120 kDa, 55 kDa
Exposure time: 1min
This image is courtesy of Stephen Yarwood, Inst Mol, Cell and Sys Bio, United KingdomImmunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
VE Cadherin immunofluorescence staining of HUVEC cells using rabbit anti-VE Cadherin antibody
ICC/IF image of VE-Cadherin staining on HUVEC cells using ab33168. The cells were incubated with the primary antibody (ab33168) and the secondary was FITC conjugated anti-rabbit used at 1:400. The cells were incubated with only the secondary antibody as a negative control.
This image is courtesy of Ana Kasirer-Friede, Univ California-San Diego, Dept. Of Medicine, United StatesImmunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
VE Cadherin immunofluorescence staining of HUVEC cells using rabbit anti-VE Cadherin antibody
ICC/IF image of VE Cadherin stained HUVEC cells. The cells were incubated with the antibody ab33168 at 1/150 dilution (Green). The cells were also stained with Rhodamine phalloidin (Red).
Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
The observed band for Cadherin 5 has a higher molecular weight of 115kDa due to glycosylation of the protein.
The immunogen used to raise this antibody has 89% homology with Cadherin 18, 88kDa , which we believe is the additional observed band at 117kDa, again due to glycosylation of the protein.
All lanes: Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168) at 1 µg/mL
All lanes: HUVEC Cell Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 88 kDa
Observed band size: 115 kDa, 117 kDa, 45 kDa
Exposure time: 1min
This image is courtesy of a customer review submitted by Kara ShumanskyImmunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
VE Cadherin immunofluorescence staining of human liver using rabbit anti-VE Cadherin antibody
ab33168 staining VE Cadherin in the endothelial cell line from Human liver by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde. Samples were incubated with primary antibody (1/100 dilution in PBS + 2.5% BSA + 0.1% triton) for 1 hour at 37°C. Alexa Fluor 594 Chicken anti-Rabbit IgG (H+L) Cross-Adsorbed Secon was used as the secondary antibody at 4 μg/ml.
This image is courtesy of a customer review submitted by Simon ShenImmunocytochemistry/ Immunofluorescence - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
VE Cadherin immunofluorescence staining of iPSC-EC using rabbit anti-VE Cadherin antibody
Immunocytochemistry analysis of formaldehyde-fixed human iPSC-EC cells staining with ab33168 at 1/250 dilution. Secondary antibody was Alexa Fluor™ 594 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed at 1/500 dilution. The cells were incubated with the primary antibody for 10 hours at 4°C. Blocking was done using 3% BSA for 1 hour at 25°C.
Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168)
Gel type: MOPS
Blocking buffer: 2% BSAAll lanes: Western blot - Anti-VE Cadherin antibody - Intercellular Junction Marker (ab33168) at 1 µg/mL
Lane 1: HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate at 10 µg
Lane 2: Mouse lung tissue lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 88 kDa
Observed band size: 120 kDa
Exposure time: 1min
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