AB119785

Anti-VE Cadherin (phospho Y685) antibody

Name: Anti-VE Cadherin (phospho Y685) antibody (ab119785) | Abcam
Brand: Abcam Limited
SKU: AB119785
Price: 470 USD
Availability: InStock
Rating: 3 (2 reviews)

(2 Reviews)

(36 Publications)

Rabbit Polyclonal VE Cadherin phospho Y685 antibody. Suitable for WB and reacts with Human samples. Cited in 36 publications. Immunogen corresponding to Synthetic Peptide within Human CDH5 phospho Y685.

View Alternative Names

CD144, Cadherin-5, 7B4 antigen, Vascular endothelial cadherin, VE-cadherin, CDH5

1 Images

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Western blot - Anti-VE Cadherin (phospho Y685) antibody (AB119785)

All lanes:

Western blot - Anti-VE Cadherin (phospho Y685) antibody (ab119785) at 1/1000 dilution

Lane 1:

Human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min.

Lane 2:

Human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min then treated with alkaline phosphatase

Predicted band size: 88 kDa

Observed band size: 140 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human CDH5 phospho Y685. The exact immunogen used to generate this antibody is proprietary information.

P33151

Specificity

ab119785 detects a 140 kDa band corresponding to VE cadherin in western blots of Human endothelial cells treated with pervanadate, and this band is not detected in untreated cells or after alkaline phosphatase treatment.

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Reactivity data

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Product details

Do not aliquot.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab119785 was cross-adsorbed to an unrelated phospho-tyrosine peptide and unphosphorylated VE Cadherin (Y685) peptide before affinity purification using phospho-VE Cadherin (Y685) peptide.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VE Cadherin also known as CD144 or Cadherin-5 is a protein of about 135 kDa involved in cell adhesion. It is a transmembrane protein characterized by its presence at endothelial cell junctions. VE Cadherin belongs to the cadherin superfamily and its expression predominantly occurs in vascular endothelial cells. This protein facilitates the adhesion between adjacent endothelial cells maintaining the integrity of endothelial barriers.

Biological function summary

VE Cadherin performs essential roles in vascular development and permeability. It is a component of adherens junctions and connects with catenins to mediate cell-cell adhesion through calcium-dependent binding. This protein's adhesive function is critical for blood vessel maturation and response to inflammatory stimuli. Altering VE Cadherin expression impacts vascular stability and endothelial cell layering.

Pathways

VE Cadherin interacts with the Wnt and VEGF signaling pathways. It plays a role in the regulation of endothelial cell survival and migration. The interaction with β-catenin is important in the Wnt pathway influencing the transcription of target genes that modulate cell behavior. In the VEGF pathway VE Cadherin's association with protein kinase C highlights its involvement in endothelial permeability and angiogenesis processes.

VE Cadherin dysregulation links to cancer progression and atherosclerosis. Its role in maintaining endothelial barrier integrity implicates it in tumor metastasis where disrupted cell adhesion facilitates cancer cell dissemination. Additionally altered VE Cadherin expression or function associates with atherosclerotic disease where damaged vascular endothelium leads to plaque formation. The connection of VE Cadherin with β-catenin in these conditions highlights its significance in pathological angiogenesis and vascular remodeling.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cadherins are calcium-dependent cell adhesion proteins (By similarity). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types (PubMed : 21269602). This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions (By similarity). It associates with alpha-catenin forming a link to the cytoskeleton (PubMed : 10861224). Plays a role in coupling actin fibers to cell junctions in endothelial cells, via acting as a cell junctional complex anchor for AMOTL2 and MAGI1 (By similarity). Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen (By similarity). These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B (PubMed : 20332120). Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction (PubMed : 20332120).

See full target information CDH5 phospho Y685

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Publications (36)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Heart and circulatory physiology 329:H671-H679 PubMed40758569

2025

Hypertensive BPH/2J mice exhibit sex-specific heart failure progression and late-life microvascular rarefaction.

Applications

Unspecified application

Species

Unspecified reactive species

Laena Pernomian,Emily W Waigi,Juliana M Parente,Cameron G McCarthy,Camilla F Wenceslau

Journal of cellular and molecular medicine 29:e70460 PubMed40074697

2025

Adrenomedullin Inhibits the Efficacy of Combined Immunotherapy and Targeted Therapy in Biliary Tract Cancer by Disrupting Endothelial Cell Functions.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengfeng Xuan,Haoran Hu,Jian Xu,Xiaowei Ling,Long Zhang,Wenzhu Li,Junda Li,Chan Zhu,Yunjie Song,Xing Zhang,Jianhua Rao,Yong Wang,Feng Cheng

Journal of applied toxicology : JAT 44:1198-1213 PubMed38639436

2024

Atorvastatin-induced intracerebral hemorrhage is inhibited by berberine in zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yan Liu,Bo Chen,Rui Zhang,Miao-Qing Zhang,Yuan-Yuan Ma,Ying Han,Jian-Dong Jiang,Jing-Pu Zhang

Journal of neuropathology and experimental neurology 83:194-204 PubMed38230623

2024

YTHDF2-regulated matrilin-3 mitigates post-reperfusion hemorrhagic transformation in ischemic stroke via the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hanze Chen,Siping Guo,Runnan Li,Lihui Yang,Rui Wang,Yasi Jiang,Yonggang Hao

The Journal of biological chemistry 299:105408 PubMed38229397

2024

Spleen Tyrosine Kinase phosphorylates VE-cadherin to cause endothelial barrier disruption in acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Shadab,Spencer A Slavin,Zahra Mahamed,Michelle W Millar,Rauf A Najar,Antony Leonard,Anthony Pietropaoli,David A Dean,Fabeha Fazal,Arshad Rahman

Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics 39:159-174 PubMed36791327

2023

Tauroursodeoxycholic Acid Alleviates Endoplasmic Reticulum Stress-Mediated Visual Deficits in Diabetic tie2-TNF Transgenic Mice via TGR5 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Raji Lenin,Kumar Abhiram Jha,Jordy Gentry,Abhishek Shrestha,Erielle V Culp,Thirumalini Vaithianathan,Rajashekhar Gangaraju

Frontiers in cell and developmental biology 11:1076250 PubMed36846596

2023

The short-chain fatty acid butyrate exerts a specific effect on VE-cadherin phosphorylation and alters the integrity of aortic endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangang Guo,Inka Terhorst,Paul Stammer,Abdulhakim Ibrahim,Alexander Oberhuber,Thorsten Eierhoff

Cellular signalling 99:110432 PubMed35933031

2022

Activating transcription factor 6 protects against endothelial barrier dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Khadeja-Tul Kubra,Mohammad S Akhter,Yogesh Saini,Konstantin G Kousoulas,Nektarios Barabutis

International journal of molecular medicine 49: PubMed35315501

2022

Rosmarinic acid downregulates the oxLDL‑induced interaction between monocytes and endothelial cells, in addition to monocyte diapedesis, under high glucose conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Jean Baptiste Nyandwi,Young Shin Ko,Hana Jin,Seung Pil Yun,Sang Won Park,Kee Ryeon Kang,Hye Jung Kim

Pharmaceuticals (Basel, Switzerland) 15: PubMed35215247

2022

Tunicamycin Protects against LPS-Induced Lung Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Khadeja-Tul Kubra,Mohammad A Uddin,Nektarios Barabutis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-VE Cadherin (phospho Y685) antibody

Western blot - Anti-VE Cadherin (phospho Y685) antibody (AB119785)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (36)

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