AB53465

Anti-VEGF 164 antibody

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(65 Publications)

Rabbit Polyclonal VEGFA antibody. Suitable for ELISA, WB, IHC-P, RIA and reacts with Rat, Pig, Human, Cow samples. Cited in 65 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Cow VEGFA.

View Alternative Names

VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF

1 Images

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Western blot - Anti-VEGF 164 antibody (AB53465)

All lanes:

Western blot - Anti-VEGF 164 antibody (ab53465) at 1/500 dilution

All lanes:

Human thymus tissue lysate - total protein (<a href='/en-us/products/unavailable/human-thymus-tissue-lysate-total-protein-ab30146'>ab30146</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa,70 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pig, Rat, Cow

Applications

RIA, ELISA, IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Cow VEGFA.

P15691

Specificity

ab53465 reacts with all human VEGF isoforms (121, 165, 189, and 206). No cross reaction is observed with PDGF, FGF-1, FGF-2, and TGFa.

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Reactivity data

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VEGF 164 also known as Vascular Endothelial Growth Factor 164 plays an important role in angiogenesis and vascular permeability. A member of the VEGF family it serves as a signal protein that promotes the growth of new blood vessels. Vegf 164 is expressed in various tissues and is specially important in the response to ischemic events and tissue remodeling. The molecular mass of VEGF 164 is approximately 45 kDa making it a protein of moderate size within its family.

Biological function summary

VEGF 164 is essential in cellular processes related to vascular growth and blood vessel formation. It is involved in endothelial cell proliferation migration and survival necessary steps for new blood vessel formation. The protein often functions as part of a larger complex that includes co-receptors like neuropilins enhancing its activity and specificity in various tissues. Overall its role is significant in maintaining the structural integrity of the vasculature.

Pathways

VEGF 164 is integrated into angiogenesis and wound healing pathways. It activates several signaling cascades including the PI3K/Akt and MAPK pathways which further involve related proteins like Akt1 and MAPK1. By mediating these pathways VEGF 164 ensures adequate nutrient and oxygen supply to growing tissues or responding to injury.

VEGF 164 has strong associations with cancer and age-related macular degeneration (AMD). In cancer it contributes to tumor growth and metastasis by aiding in the construction of new blood vessels that supply tumor cells. In AMD VEGF 164 levels can increase leading to abnormal blood vessel growth in the eye damaging vision. The protein interacts with similar function proteins like VEGF receptor 2 which is important in same pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed : 35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).. VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed : 34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437).. Isoform VEGF165B. Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

See full target information VEGFA

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Publications (65)

Recent publications for all applications. Explore the full list and refine your search

Life (Basel, Switzerland) 14: PubMed38255715

2024

Special Staining and Protein Expression of VEGF/EGFR and P53/NF-κB in Cryptorchid Tissue of Erhualian Pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Penggang Liu,Yiming Shao,Caihong Liu,Xiaoyang Lv,Seth Yaw Afedo,Wenbin Bao

Biomedicines 11: PubMed37239014

2023

Anti-Fibrotic Efficacy of Apigenin in a Mice Model of Carbon Tetrachloride-Induced Hepatic Fibrosis by Modulation of Oxidative Stress, Inflammation, and Fibrogenesis: A Preclinical Study.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam Melaibari,Huda M Alkreathy,Ahmed Esmat,Nisreen A Rajeh,Rasheed A Shaik,Anwar A Alghamdi,Aftab Ahmad

Evidence-based complementary and alternative medicine : eCAM 2022:5183809 PubMed35783525

2022

Therapeutic Effect and Mechanism of Negative Pressure Wound Therapy with Huoxue Shengji Decoction Instillation for Chronic Skin Ulcers.

Applications

Unspecified application

Species

Unspecified reactive species

Sunfeng Pan,Lie Xiong,Zhenjun Wang,Yujuan Su,Gaofeng Fang,Minda Zhu,Jing Wang,Hanqiang Shi,Shihui Zhu,Yanbo Shi

Journal of materials science. Materials in medicine 33:19 PubMed35072831

2022

Loading of erythropoietin on biphasic calcium phosphate bioceramics promotes osteogenesis and angiogenesis by regulating EphB4/EphrinB2 molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Peng Wang,Qionghui Wu,Zhifan Qin,Zichao Xiang,Yuxian Chu,Jihua Li

Pharmaceutical biology 59:1566-1575 PubMed34767490

2021

Gambogic amide inhibits angiogenesis by suppressing VEGF/VEGFR2 in endothelial cells in a TrkA-independent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Tongtong Sui,Bojun Qiu,Jiaorong Qu,Yuxin Wang,Kunnian Ran,Wei Han,Xiaozhong Peng

International journal of nanomedicine 16:3889-3905 PubMed34135583

2021

Wound Healing Activity of Fixed Oil Formulated in a Self-Nanoemulsifying Formulation.

Applications

Unspecified application

Species

Unspecified reactive species

Abdulrahman E Koshak,Mardi M Algandaby,Mohammad I Mujallid,Ashraf B Abdel-Naim,Nabil A Alhakamy,Usama A Fahmy,Anas Alfarsi,Shaimaa M Badr-Eldin,Thikryat Neamatallah,Mohammed Z Nasrullah,Hossam M Abdallah,Ahmed Esmat

Molecular medicine reports 23: PubMed33760199

2021

Serum from patients with hypertension promotes endothelial dysfunction to induce trophoblast invasion through the miR‑27b‑3p/ATPase plasma membrane Ca transporting 1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Libo Zhu,Zhuqing Liu

Frontiers in pharmacology 11:547436 PubMed33584252

2021

EFEMP1 Overexpression Contributes to Neovascularization in Age-Related Macular Degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Cheng,Chong Chen,Wenke Guo,Kun Liu,Qianqian Zhao,Ping Lu,Fudong Yu,Xun Xu

Journal of orthopaedic surgery and research 16:71 PubMed33472642

2021

miRNA-128 modulates bone neoplasms cells proliferation and migration through the WNT/β-catenin and EMT signal pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Xiaotao Long,Ji Wang,Jing Peng,Kai Shen

International journal of molecular medicine 47:732-740 PubMed33416127

2021

ASK1/p38‑mediated NLRP3 inflammasome signaling pathway contributes to aberrant retinal angiogenesis in diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjun Zou,Shasha Luo,Zhengwei Zhang,Libo Cheng,Xiaoli Huang,Nannan Ding,Ying Pan,Zhifeng Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VEGF 164 antibody

Western blot - Anti-VEGF 164 antibody (AB53465)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (65)

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